Isolation and characterization of phenanthrene degrading bacteria from diesel fuel-contaminated antarctic soils
Antarctica is an attractive target for human exploration and scientific investigation, however the negative effects of human activity on this continent are long lasting and can have serious consequences on the native ecosystem. Various areas of Antarctica have been contaminated with diesel fuel, whi...
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ftunivchile:oai:repositorio.uchile.cl:2250/149669 2023-05-15T13:41:41+02:00 Isolation and characterization of phenanthrene degrading bacteria from diesel fuel-contaminated antarctic soils Gran Scheuch, Alejandro Alberto Fuentes Pérez, Edwar Bravo Rodríguez, Denisse Cristobal Jimenez, Juan Pérez Donoso, José 2017 application/pdf https://doi.org/10.3389/fmicb.2017.01634 https://repositorio.uchile.cl/handle/2250/149669 en eng Frontiers media SA Front. Microbiol. 8:1634 doi:10.3389/fmicb.2017.01634 https://repositorio.uchile.cl/handle/2250/149669 Attribution-NonCommercial-NoDerivs 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ CC-BY-NC-ND Frontiers in Microbiology Antarctica Bioremediation Phenanthrene Artículo de revista 2017 ftunivchile https://doi.org/10.3389/fmicb.2017.01634 2023-01-22T01:05:11Z Antarctica is an attractive target for human exploration and scientific investigation, however the negative effects of human activity on this continent are long lasting and can have serious consequences on the native ecosystem. Various areas of Antarctica have been contaminated with diesel fuel, which contains harmful compounds such as heavy metals and polycyclic aromatic hydrocarbons (PAH). Bioremediation of PAHs by the activity of microorganisms is an ecological, economical, and safe decontamination approach. Since the introduction of foreign organisms into the Antarctica is prohibited, it is key to discover native bacteria that can be used for diesel bioremediation. By following the degradation of the PAH phenanthrene, we isolated 53 PAH metabolizing bacteria from diesel contaminated Antarctic soil samples, with three of these isolates exhibiting a high phenanthrene degrading capacity. In particular, the Sphingobium xenophagum D43FB isolate showed the highest phenanthrene degradation ability, generating up to 95% degradation of initial phenanthrene. D43FB can also degrade phenanthrene in the presence of its usual co-pollutant, the heavy metal cadmium, and showed the ability to grow using diesel-fuel as a sole carbon source. Microtiter plate assays and SEM analysis revealed that S. xenophagum D43FB exhibits the ability to form biofilms and can directly adhere to phenanthrene crystals. Genome sequencing analysis also revealed the presence of several genes involved in PAH degradation and heavy metal resistance in the D43FB genome. Altogether, these results demonstrate that S. xenophagum D43FB shows promising potential for its application in the bioremediation of diesel fuel contaminated-Antarctic ecosy stems. Erika Elcira Donoso Lopez Fondecyt 1151255 INACH MT-05_13 INACH RT-25_16 UNAB DI 488-14/R AFOSR FA9550-15-1-0140 CONICYT (Comision Nacional de Investigacion Cientifica y Tecnologica) Article in Journal/Newspaper Antarc* Antarctic Antarctica Universidad de Chile: Repositorio académico Antarctic Lopez ENVELOPE(-63.567,-63.567,-64.850,-64.850) Inach ENVELOPE(-60.783,-60.783,-62.467,-62.467) Frontiers in Microbiology 8 |
institution |
Open Polar |
collection |
Universidad de Chile: Repositorio académico |
op_collection_id |
ftunivchile |
language |
English |
topic |
Antarctica Bioremediation Phenanthrene |
spellingShingle |
Antarctica Bioremediation Phenanthrene Gran Scheuch, Alejandro Alberto Fuentes Pérez, Edwar Bravo Rodríguez, Denisse Cristobal Jimenez, Juan Pérez Donoso, José Isolation and characterization of phenanthrene degrading bacteria from diesel fuel-contaminated antarctic soils |
topic_facet |
Antarctica Bioremediation Phenanthrene |
description |
Antarctica is an attractive target for human exploration and scientific investigation, however the negative effects of human activity on this continent are long lasting and can have serious consequences on the native ecosystem. Various areas of Antarctica have been contaminated with diesel fuel, which contains harmful compounds such as heavy metals and polycyclic aromatic hydrocarbons (PAH). Bioremediation of PAHs by the activity of microorganisms is an ecological, economical, and safe decontamination approach. Since the introduction of foreign organisms into the Antarctica is prohibited, it is key to discover native bacteria that can be used for diesel bioremediation. By following the degradation of the PAH phenanthrene, we isolated 53 PAH metabolizing bacteria from diesel contaminated Antarctic soil samples, with three of these isolates exhibiting a high phenanthrene degrading capacity. In particular, the Sphingobium xenophagum D43FB isolate showed the highest phenanthrene degradation ability, generating up to 95% degradation of initial phenanthrene. D43FB can also degrade phenanthrene in the presence of its usual co-pollutant, the heavy metal cadmium, and showed the ability to grow using diesel-fuel as a sole carbon source. Microtiter plate assays and SEM analysis revealed that S. xenophagum D43FB exhibits the ability to form biofilms and can directly adhere to phenanthrene crystals. Genome sequencing analysis also revealed the presence of several genes involved in PAH degradation and heavy metal resistance in the D43FB genome. Altogether, these results demonstrate that S. xenophagum D43FB shows promising potential for its application in the bioremediation of diesel fuel contaminated-Antarctic ecosy stems. Erika Elcira Donoso Lopez Fondecyt 1151255 INACH MT-05_13 INACH RT-25_16 UNAB DI 488-14/R AFOSR FA9550-15-1-0140 CONICYT (Comision Nacional de Investigacion Cientifica y Tecnologica) |
format |
Article in Journal/Newspaper |
author |
Gran Scheuch, Alejandro Alberto Fuentes Pérez, Edwar Bravo Rodríguez, Denisse Cristobal Jimenez, Juan Pérez Donoso, José |
author_facet |
Gran Scheuch, Alejandro Alberto Fuentes Pérez, Edwar Bravo Rodríguez, Denisse Cristobal Jimenez, Juan Pérez Donoso, José |
author_sort |
Gran Scheuch, Alejandro Alberto |
title |
Isolation and characterization of phenanthrene degrading bacteria from diesel fuel-contaminated antarctic soils |
title_short |
Isolation and characterization of phenanthrene degrading bacteria from diesel fuel-contaminated antarctic soils |
title_full |
Isolation and characterization of phenanthrene degrading bacteria from diesel fuel-contaminated antarctic soils |
title_fullStr |
Isolation and characterization of phenanthrene degrading bacteria from diesel fuel-contaminated antarctic soils |
title_full_unstemmed |
Isolation and characterization of phenanthrene degrading bacteria from diesel fuel-contaminated antarctic soils |
title_sort |
isolation and characterization of phenanthrene degrading bacteria from diesel fuel-contaminated antarctic soils |
publisher |
Frontiers media SA |
publishDate |
2017 |
url |
https://doi.org/10.3389/fmicb.2017.01634 https://repositorio.uchile.cl/handle/2250/149669 |
long_lat |
ENVELOPE(-63.567,-63.567,-64.850,-64.850) ENVELOPE(-60.783,-60.783,-62.467,-62.467) |
geographic |
Antarctic Lopez Inach |
geographic_facet |
Antarctic Lopez Inach |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Frontiers in Microbiology |
op_relation |
Front. Microbiol. 8:1634 doi:10.3389/fmicb.2017.01634 https://repositorio.uchile.cl/handle/2250/149669 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.3389/fmicb.2017.01634 |
container_title |
Frontiers in Microbiology |
container_volume |
8 |
_version_ |
1766153808173334528 |