Biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant Antarctic bacteria: exploring novel natural nanofactories
Background: Fluorescent nanoparticles or quantum dots (QDs) have been intensely studied for basic and applied research due to their unique size-dependent properties. There is an increasing interest in developing ecofriendly methods to synthesize these nanoparticles since they improve biocompatibilit...
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ftunivchile:oai:repositorio.uchile.cl:2250/140757 2023-05-15T13:59:14+02:00 Biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant Antarctic bacteria: exploring novel natural nanofactories Plaza, D. O. Gallardo, C. Straub, Y. D. Bravo, D. Pérez Donoso, José 2016 application/pdf https://doi.org/10.1186/s12934-016-0477-8 https://repositorio.uchile.cl/handle/2250/140757 en eng Biomed Central Microb Cell Fact (2016) 15:76 doi:10.1186/s12934-016-0477-8 https://repositorio.uchile.cl/handle/2250/140757 Attribution-NonCommercial-NoDerivs 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ CC-BY-NC-ND Microbial Cell Factories Fluorescent nanoparticles Quantum dots Green synthesis Antarctica Bacteria Heavy metals Artículo de revista 2016 ftunivchile https://doi.org/10.1186/s12934-016-0477-8 2023-01-22T00:55:03Z Background: Fluorescent nanoparticles or quantum dots (QDs) have been intensely studied for basic and applied research due to their unique size-dependent properties. There is an increasing interest in developing ecofriendly methods to synthesize these nanoparticles since they improve biocompatibility and avoid the generation of toxic byproducts. The use of biological systems, particularly prokaryotes, has emerged as a promising alternative. Recent studies indicate that QDs biosynthesis is related to factors such as cellular redox status and antioxidant defenses. Based on this, the mixture of extreme conditions of Antarctica would allow the development of natural QDs producing bacteria. Results: In this study we isolated and characterized cadmium and tellurite resistant Antarctic bacteria capable of synthesizing CdS and CdTe QDs when exposed to these oxidizing heavy metals. A time dependent change in fluorescence emission color, moving from green to red, was determined on bacterial cells exposed to metals. Biosynthesis was observed in cells grown at different temperatures and high metal concentrations. Electron microscopy analysis of treated cells revealed nanometric electron-dense elements and structures resembling membrane vesicles mostly associated to periplasmic space. Purified biosynthesized QDs displayed broad absorption and emission spectra characteristic of biogenic Cd nanoparticles. Conclusions: Our work presents a novel and simple biological approach to produce QDs at room temperature by using heavy metal resistant Antarctic bacteria, highlighting the unique properties of these microorganisms as potent natural producers of nano-scale materials and promising candidates for bioremediation purposes. Fondecyt 1151255 11110076 Anillo ACT 1111 1107 INACH Grant T_19-11 MG_01-13 Programa de Formacion de Capital Humano Avanzado de CONICYT Article in Journal/Newspaper Antarc* Antarctic Antarctica Universidad de Chile: Repositorio académico Antarctic Inach ENVELOPE(-60.783,-60.783,-62.467,-62.467) Microbial Cell Factories 15 1 |
institution |
Open Polar |
collection |
Universidad de Chile: Repositorio académico |
op_collection_id |
ftunivchile |
language |
English |
topic |
Fluorescent nanoparticles Quantum dots Green synthesis Antarctica Bacteria Heavy metals |
spellingShingle |
Fluorescent nanoparticles Quantum dots Green synthesis Antarctica Bacteria Heavy metals Plaza, D. O. Gallardo, C. Straub, Y. D. Bravo, D. Pérez Donoso, José Biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant Antarctic bacteria: exploring novel natural nanofactories |
topic_facet |
Fluorescent nanoparticles Quantum dots Green synthesis Antarctica Bacteria Heavy metals |
description |
Background: Fluorescent nanoparticles or quantum dots (QDs) have been intensely studied for basic and applied research due to their unique size-dependent properties. There is an increasing interest in developing ecofriendly methods to synthesize these nanoparticles since they improve biocompatibility and avoid the generation of toxic byproducts. The use of biological systems, particularly prokaryotes, has emerged as a promising alternative. Recent studies indicate that QDs biosynthesis is related to factors such as cellular redox status and antioxidant defenses. Based on this, the mixture of extreme conditions of Antarctica would allow the development of natural QDs producing bacteria. Results: In this study we isolated and characterized cadmium and tellurite resistant Antarctic bacteria capable of synthesizing CdS and CdTe QDs when exposed to these oxidizing heavy metals. A time dependent change in fluorescence emission color, moving from green to red, was determined on bacterial cells exposed to metals. Biosynthesis was observed in cells grown at different temperatures and high metal concentrations. Electron microscopy analysis of treated cells revealed nanometric electron-dense elements and structures resembling membrane vesicles mostly associated to periplasmic space. Purified biosynthesized QDs displayed broad absorption and emission spectra characteristic of biogenic Cd nanoparticles. Conclusions: Our work presents a novel and simple biological approach to produce QDs at room temperature by using heavy metal resistant Antarctic bacteria, highlighting the unique properties of these microorganisms as potent natural producers of nano-scale materials and promising candidates for bioremediation purposes. Fondecyt 1151255 11110076 Anillo ACT 1111 1107 INACH Grant T_19-11 MG_01-13 Programa de Formacion de Capital Humano Avanzado de CONICYT |
format |
Article in Journal/Newspaper |
author |
Plaza, D. O. Gallardo, C. Straub, Y. D. Bravo, D. Pérez Donoso, José |
author_facet |
Plaza, D. O. Gallardo, C. Straub, Y. D. Bravo, D. Pérez Donoso, José |
author_sort |
Plaza, D. O. |
title |
Biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant Antarctic bacteria: exploring novel natural nanofactories |
title_short |
Biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant Antarctic bacteria: exploring novel natural nanofactories |
title_full |
Biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant Antarctic bacteria: exploring novel natural nanofactories |
title_fullStr |
Biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant Antarctic bacteria: exploring novel natural nanofactories |
title_full_unstemmed |
Biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant Antarctic bacteria: exploring novel natural nanofactories |
title_sort |
biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant antarctic bacteria: exploring novel natural nanofactories |
publisher |
Biomed Central |
publishDate |
2016 |
url |
https://doi.org/10.1186/s12934-016-0477-8 https://repositorio.uchile.cl/handle/2250/140757 |
long_lat |
ENVELOPE(-60.783,-60.783,-62.467,-62.467) |
geographic |
Antarctic Inach |
geographic_facet |
Antarctic Inach |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Microbial Cell Factories |
op_relation |
Microb Cell Fact (2016) 15:76 doi:10.1186/s12934-016-0477-8 https://repositorio.uchile.cl/handle/2250/140757 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1186/s12934-016-0477-8 |
container_title |
Microbial Cell Factories |
container_volume |
15 |
container_issue |
1 |
_version_ |
1766267744017186816 |