High-throughput transcriptome analysis of ISAV-infected Atlantic salmon Salmo salar unravels divergent immune responses associated to head-kidney, liver and gills tissues
Artículo de publicación ISI Infectious salmon anaemia virus (ISAV) is an orthomyxovirus causing high mortality in farmed Atlantic salmon (Salmo salar). The collective data from the Atlantic salmoneISAV interactions, performed “in vitro” using various salmon cell lines and “in vivo” fish infected wit...
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ftunivchile:oai:repositorio.uchile.cl:2250/134705 2023-05-15T15:31:50+02:00 High-throughput transcriptome analysis of ISAV-infected Atlantic salmon Salmo salar unravels divergent immune responses associated to head-kidney, liver and gills tissues Valenzuela Miranda, Diego Boltaña, Sebastián Cabrejos, María Eugenia Yáñez López, José Gallardo Escárate, Cristian 2015 application/pdf https://doi.org/10.1016/j.fsi.2015.04.003 https://repositorio.uchile.cl/handle/2250/134705 en eng Elsevier Fish & Shellfish Immunology 45 (2015) 367-377 doi:10.1016/j.fsi.2015.04.003 https://repositorio.uchile.cl/handle/2250/134705 Atribución-NoComercial-SinDerivadas 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ CC-BY-NC-ND RNA-seq ISAV Viral segments rt-qPCR Salmo salar Artículo de revista 2015 ftunivchile https://doi.org/10.1016/j.fsi.2015.04.003 2023-01-29T00:54:27Z Artículo de publicación ISI Infectious salmon anaemia virus (ISAV) is an orthomyxovirus causing high mortality in farmed Atlantic salmon (Salmo salar). The collective data from the Atlantic salmoneISAV interactions, performed “in vitro” using various salmon cell lines and “in vivo” fish infected with different ISAV isolates, have shown a strong regulation of immune related transcripts during the infection. Despite this strong defence response, the majority of fish succumb to infections with ISAV. The deficient protection of the host against ISAV is in part due to virulence factors of the virus, which allow evade the host-defence machinery. As such, the viral replication is uninhibited and viral loads quickly spread to several tissues causing massive cellular damage before the host can develop an effective cell-mediated and humoral outcome. To interrogate the correlation of the viral replication with the host defence response, we used fish that have been infected by cohabitation with ISAV-injected salmons. Whole gene expression patterns were measured with RNA-seq using RNA extracted from Head-kidney, Liver and Gills. The results show divergent mRNA abundance of functional modules related to interferon pathway, adaptive/ innate immune response and cellular proliferation/differentiation. Furthermore, gene regulation in distinct tissues during the infection process was independently controlled within the each tissue and the observed mRNA expression suggests high modulation of the ISAV-segment transcription. Importantly this is the first time that strong correlations between functional modules containing significant immune process with proteineprotein affinities and viral-segment transcription have been made between different tissues of ISAV-infected fish. FONDAP project 1510027 CORFO INNOVA grant 09-MCSS6682 Article in Journal/Newspaper Atlantic salmon Salmo salar Universidad de Chile: Repositorio académico Isi ENVELOPE(-38.550,-38.550,65.617,65.617) Fish & Shellfish Immunology 45 2 367 377 |
institution |
Open Polar |
collection |
Universidad de Chile: Repositorio académico |
op_collection_id |
ftunivchile |
language |
English |
topic |
RNA-seq ISAV Viral segments rt-qPCR Salmo salar |
spellingShingle |
RNA-seq ISAV Viral segments rt-qPCR Salmo salar Valenzuela Miranda, Diego Boltaña, Sebastián Cabrejos, María Eugenia Yáñez López, José Gallardo Escárate, Cristian High-throughput transcriptome analysis of ISAV-infected Atlantic salmon Salmo salar unravels divergent immune responses associated to head-kidney, liver and gills tissues |
topic_facet |
RNA-seq ISAV Viral segments rt-qPCR Salmo salar |
description |
Artículo de publicación ISI Infectious salmon anaemia virus (ISAV) is an orthomyxovirus causing high mortality in farmed Atlantic salmon (Salmo salar). The collective data from the Atlantic salmoneISAV interactions, performed “in vitro” using various salmon cell lines and “in vivo” fish infected with different ISAV isolates, have shown a strong regulation of immune related transcripts during the infection. Despite this strong defence response, the majority of fish succumb to infections with ISAV. The deficient protection of the host against ISAV is in part due to virulence factors of the virus, which allow evade the host-defence machinery. As such, the viral replication is uninhibited and viral loads quickly spread to several tissues causing massive cellular damage before the host can develop an effective cell-mediated and humoral outcome. To interrogate the correlation of the viral replication with the host defence response, we used fish that have been infected by cohabitation with ISAV-injected salmons. Whole gene expression patterns were measured with RNA-seq using RNA extracted from Head-kidney, Liver and Gills. The results show divergent mRNA abundance of functional modules related to interferon pathway, adaptive/ innate immune response and cellular proliferation/differentiation. Furthermore, gene regulation in distinct tissues during the infection process was independently controlled within the each tissue and the observed mRNA expression suggests high modulation of the ISAV-segment transcription. Importantly this is the first time that strong correlations between functional modules containing significant immune process with proteineprotein affinities and viral-segment transcription have been made between different tissues of ISAV-infected fish. FONDAP project 1510027 CORFO INNOVA grant 09-MCSS6682 |
format |
Article in Journal/Newspaper |
author |
Valenzuela Miranda, Diego Boltaña, Sebastián Cabrejos, María Eugenia Yáñez López, José Gallardo Escárate, Cristian |
author_facet |
Valenzuela Miranda, Diego Boltaña, Sebastián Cabrejos, María Eugenia Yáñez López, José Gallardo Escárate, Cristian |
author_sort |
Valenzuela Miranda, Diego |
title |
High-throughput transcriptome analysis of ISAV-infected Atlantic salmon Salmo salar unravels divergent immune responses associated to head-kidney, liver and gills tissues |
title_short |
High-throughput transcriptome analysis of ISAV-infected Atlantic salmon Salmo salar unravels divergent immune responses associated to head-kidney, liver and gills tissues |
title_full |
High-throughput transcriptome analysis of ISAV-infected Atlantic salmon Salmo salar unravels divergent immune responses associated to head-kidney, liver and gills tissues |
title_fullStr |
High-throughput transcriptome analysis of ISAV-infected Atlantic salmon Salmo salar unravels divergent immune responses associated to head-kidney, liver and gills tissues |
title_full_unstemmed |
High-throughput transcriptome analysis of ISAV-infected Atlantic salmon Salmo salar unravels divergent immune responses associated to head-kidney, liver and gills tissues |
title_sort |
high-throughput transcriptome analysis of isav-infected atlantic salmon salmo salar unravels divergent immune responses associated to head-kidney, liver and gills tissues |
publisher |
Elsevier |
publishDate |
2015 |
url |
https://doi.org/10.1016/j.fsi.2015.04.003 https://repositorio.uchile.cl/handle/2250/134705 |
long_lat |
ENVELOPE(-38.550,-38.550,65.617,65.617) |
geographic |
Isi |
geographic_facet |
Isi |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Fish & Shellfish Immunology 45 (2015) 367-377 doi:10.1016/j.fsi.2015.04.003 https://repositorio.uchile.cl/handle/2250/134705 |
op_rights |
Atribución-NoComercial-SinDerivadas 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.fsi.2015.04.003 |
container_title |
Fish & Shellfish Immunology |
container_volume |
45 |
container_issue |
2 |
container_start_page |
367 |
op_container_end_page |
377 |
_version_ |
1766362345798369280 |