Identification and analysis of metabolite production with biotechnological potential in Xanthophyllomyces dendrorhous isolates
Artículo de publicación ISI Abstract Antarctic microorganisms have developed different strategies to live in their environments, including modifications to their membrane components to regulate fluidity and the production of photoprotective metabolites such as carotenoids. Three yeast colonies (ANCH...
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ftunivchile:oai:repositorio.uchile.cl:2250/133194 2023-05-15T13:47:20+02:00 Identification and analysis of metabolite production with biotechnological potential in Xanthophyllomyces dendrorhous isolates Contreras, Gabriela Barahona‑Crisóstomo, Salvador Sepúlveda, Dionisia Baeza Cancino, Marcelo 2015 application/pdf https://doi.org/10.1007/s11274 https://repositorio.uchile.cl/handle/2250/133194 en eng Springer World J Microbiol Biotechnol (2015) 31:517–526 doi:10.1007/s11274 https://repositorio.uchile.cl/handle/2250/133194 Atribución-NoComercial-SinDerivadas 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ CC-BY-NC-ND Polyunsaturated fatty acids Phaffia rhodozyma Xanthophyllomyces dendrorhous Astaxanthin Carotenoids Artículo de revista 2015 ftunivchile https://doi.org/10.1007/s11274 2023-03-05T00:54:31Z Artículo de publicación ISI Abstract Antarctic microorganisms have developed different strategies to live in their environments, including modifications to their membrane components to regulate fluidity and the production of photoprotective metabolites such as carotenoids. Three yeast colonies (ANCH01, ANCH06 and ANCH08) were isolated from soil samples collected at King George Island, which according to their rDNA sequence analyses, were determined to be Xanthophyllomyces dendrorhous. This yeast is of biotechnological interest, because it can synthesize astaxanthin as its main carotenoid, which is a powerful antioxidant pigment used in aquaculture. Then, the aim of this work was to characterize the ANCH isolates at their molecular and phenotypic level. The isolates did not display any differences in their rDNA and COX1 gene nucleotide sequences. However, ANCH01 produces approximately sixfold more astaxanthin than other wild type strains. Moreover, even though ANCH06 and ANCH08 produce astaxanthin, their main carotenoid was b-carotene. In contrast to other X. dendrorhous strains, the ANCH isolates did not produce mycosporines. Finally, the ANCH isolates had a higher proportion of polyunsaturated fatty acids than other wild type strains. In conclusion, the reported X. dendrorhous isolates are phenotypically different from other wild type strains, including characteristics that could make them more resistant and better able to inhabit their original habitat, which may also have biotechnological potential. CONICYT Article in Journal/Newspaper Antarc* Antarctic King George Island Universidad de Chile: Repositorio académico Antarctic Isi ENVELOPE(-38.550,-38.550,65.617,65.617) King George Island |
institution |
Open Polar |
collection |
Universidad de Chile: Repositorio académico |
op_collection_id |
ftunivchile |
language |
English |
topic |
Polyunsaturated fatty acids Phaffia rhodozyma Xanthophyllomyces dendrorhous Astaxanthin Carotenoids |
spellingShingle |
Polyunsaturated fatty acids Phaffia rhodozyma Xanthophyllomyces dendrorhous Astaxanthin Carotenoids Contreras, Gabriela Barahona‑Crisóstomo, Salvador Sepúlveda, Dionisia Baeza Cancino, Marcelo Identification and analysis of metabolite production with biotechnological potential in Xanthophyllomyces dendrorhous isolates |
topic_facet |
Polyunsaturated fatty acids Phaffia rhodozyma Xanthophyllomyces dendrorhous Astaxanthin Carotenoids |
description |
Artículo de publicación ISI Abstract Antarctic microorganisms have developed different strategies to live in their environments, including modifications to their membrane components to regulate fluidity and the production of photoprotective metabolites such as carotenoids. Three yeast colonies (ANCH01, ANCH06 and ANCH08) were isolated from soil samples collected at King George Island, which according to their rDNA sequence analyses, were determined to be Xanthophyllomyces dendrorhous. This yeast is of biotechnological interest, because it can synthesize astaxanthin as its main carotenoid, which is a powerful antioxidant pigment used in aquaculture. Then, the aim of this work was to characterize the ANCH isolates at their molecular and phenotypic level. The isolates did not display any differences in their rDNA and COX1 gene nucleotide sequences. However, ANCH01 produces approximately sixfold more astaxanthin than other wild type strains. Moreover, even though ANCH06 and ANCH08 produce astaxanthin, their main carotenoid was b-carotene. In contrast to other X. dendrorhous strains, the ANCH isolates did not produce mycosporines. Finally, the ANCH isolates had a higher proportion of polyunsaturated fatty acids than other wild type strains. In conclusion, the reported X. dendrorhous isolates are phenotypically different from other wild type strains, including characteristics that could make them more resistant and better able to inhabit their original habitat, which may also have biotechnological potential. CONICYT |
format |
Article in Journal/Newspaper |
author |
Contreras, Gabriela Barahona‑Crisóstomo, Salvador Sepúlveda, Dionisia Baeza Cancino, Marcelo |
author_facet |
Contreras, Gabriela Barahona‑Crisóstomo, Salvador Sepúlveda, Dionisia Baeza Cancino, Marcelo |
author_sort |
Contreras, Gabriela |
title |
Identification and analysis of metabolite production with biotechnological potential in Xanthophyllomyces dendrorhous isolates |
title_short |
Identification and analysis of metabolite production with biotechnological potential in Xanthophyllomyces dendrorhous isolates |
title_full |
Identification and analysis of metabolite production with biotechnological potential in Xanthophyllomyces dendrorhous isolates |
title_fullStr |
Identification and analysis of metabolite production with biotechnological potential in Xanthophyllomyces dendrorhous isolates |
title_full_unstemmed |
Identification and analysis of metabolite production with biotechnological potential in Xanthophyllomyces dendrorhous isolates |
title_sort |
identification and analysis of metabolite production with biotechnological potential in xanthophyllomyces dendrorhous isolates |
publisher |
Springer |
publishDate |
2015 |
url |
https://doi.org/10.1007/s11274 https://repositorio.uchile.cl/handle/2250/133194 |
long_lat |
ENVELOPE(-38.550,-38.550,65.617,65.617) |
geographic |
Antarctic Isi King George Island |
geographic_facet |
Antarctic Isi King George Island |
genre |
Antarc* Antarctic King George Island |
genre_facet |
Antarc* Antarctic King George Island |
op_relation |
World J Microbiol Biotechnol (2015) 31:517–526 doi:10.1007/s11274 https://repositorio.uchile.cl/handle/2250/133194 |
op_rights |
Atribución-NoComercial-SinDerivadas 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1007/s11274 |
_version_ |
1766246934823043072 |