Polyphasic identification methods applied in searching for microorganisms with industrial potential
Background: The bioprospection of metabolites of anthropogenic interests employs methods of collecting microorganisms in extremophile or endemic ecosystems. The microbiota isolated in these places may or may not include pathogenic microorganisms. Therefore, an interdisciplinary approach is essential...
Published in: | Revista de la Facultad de Ciencias Médicas (Quito) |
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Universidad Central del Ecuador
2020
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Online Access: | https://revistadigital.uce.edu.ec/index.php/CIENCIAS_MEDICAS/article/view/2687 https://doi.org/10.29166/rfcmq.v44i2.2687 |
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ftunivcecuojs:oai:revistadigital.uce.edu.ec:article/2687 2024-09-15T17:41:44+00:00 Polyphasic identification methods applied in searching for microorganisms with industrial potential Métodos de identificación polifásica aplicados a la búsqueda de microorganismos con potencial industrial Salvador Pinos, Carmen Herrera Naranjo, FNicolás Michelena Tupiza, Stephanie González Gavilanez, Humberto Gualoto Oñate, Miguel González Suárez, Erenio Mesa Garriga, Leyanis Rojas Carrillo, Maira 2020-11-18 application/pdf https://revistadigital.uce.edu.ec/index.php/CIENCIAS_MEDICAS/article/view/2687 https://doi.org/10.29166/rfcmq.v44i2.2687 spa spa Universidad Central del Ecuador https://revistadigital.uce.edu.ec/index.php/CIENCIAS_MEDICAS/article/view/2687/3101 https://revistadigital.uce.edu.ec/index.php/CIENCIAS_MEDICAS/article/view/2687 doi:10.29166/rfcmq.v44i2.2687 Derechos de autor 2020 Carmen Salvador Pinos, FNicolás Herrera Naranjo, Stephanie Michelena Tupiza, Humberto González Gavilanez, Miguel Gualoto Oñate, Erenio González Suárez, Leyanis Mesa Garriga, Maira Rojas Carrillo http://creativecommons.org/licenses/by-nc-nd/4.0 Revista de la Facultad de Ciencias Médicas (Quito); Vol. 44 Núm. 2 (2019): Revista de la Facultad de Ciencias Médicas (Quito); 10 - 23 2737-6141 2588-0691 Microorganismos patógenos microorganismos Antártida identificación molecular 16S bioprospección : Pathogenic microorganisms Antarctic microorganisms molecular identification bioprospecting info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo evaluado por pares 2020 ftunivcecuojs https://doi.org/10.29166/rfcmq.v44i2.2687 2024-08-06T03:08:43Z Background: The bioprospection of metabolites of anthropogenic interests employs methods of collecting microorganisms in extremophile or endemic ecosystems. The microbiota isolated in these places may or may not include pathogenic microorganisms. Therefore, an interdisciplinary approach is essential to address the search of the species of interest while the good health of the researchers is preserved. Objective: To identify in molecular, biochemical and morphologically ways some human pathogenic microorganisms in cellulolytic strains of industrial importance stored in the strain bank of the Research Laboratory of the Faculty of Chemical Engineering at the Central University of Ecuador, from Yasuní, Antarctic and Balzapamba. Methods: IA bioprospecting study of cellulolytic bacteria was performed using environmental microbiology techniques. Morphological characteristics were assessed by Gram staining. In addition, biochemical tests and antibiograms were performed for Gram-negative and Gram-positive bacteria. The molecular tests used extraction of bacterial ADN for 16S gene Sanger sequencing. Results: The species Klebsiella pneumoniae (Y2 and Y3r) and Nocardia asteroides (Y1 and Y3p) were found in samples of lignocellulosic material collected in Yasuni, while the isolated species in Antarctica and Balzapamba correspond to Bacillus subtillis. Conclusions: Strains belonging to different bacterial genera were identified. The bacteria of the genus Klebsiella from the samples collected in Yasuní could have a potential pathogen. This can be corroborated with genotyping techniques. Therefore, there could be a risk to humans who perform bioprospecting in that ecosystem and biosecurity measures should be taken. Introducción: La bioprospección de metabolitos de interés antropogénico emplea métodos de recolección de microorganismos en ecosistemas extremófilos o endémicos. La microbiota aislada en estos lugares puede o no incluir microorganismos patógenos. Es imprescindible un enfoque interdisciplinario que permita abordar ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Antártida Portal de Revistas Universidad Central del Ecuador Revista de la Facultad de Ciencias Médicas (Quito) 44 2 10 23 |
institution |
Open Polar |
collection |
Portal de Revistas Universidad Central del Ecuador |
op_collection_id |
ftunivcecuojs |
language |
Spanish |
topic |
Microorganismos patógenos microorganismos Antártida identificación molecular 16S bioprospección : Pathogenic microorganisms Antarctic microorganisms molecular identification bioprospecting |
spellingShingle |
Microorganismos patógenos microorganismos Antártida identificación molecular 16S bioprospección : Pathogenic microorganisms Antarctic microorganisms molecular identification bioprospecting Salvador Pinos, Carmen Herrera Naranjo, FNicolás Michelena Tupiza, Stephanie González Gavilanez, Humberto Gualoto Oñate, Miguel González Suárez, Erenio Mesa Garriga, Leyanis Rojas Carrillo, Maira Polyphasic identification methods applied in searching for microorganisms with industrial potential |
topic_facet |
Microorganismos patógenos microorganismos Antártida identificación molecular 16S bioprospección : Pathogenic microorganisms Antarctic microorganisms molecular identification bioprospecting |
description |
Background: The bioprospection of metabolites of anthropogenic interests employs methods of collecting microorganisms in extremophile or endemic ecosystems. The microbiota isolated in these places may or may not include pathogenic microorganisms. Therefore, an interdisciplinary approach is essential to address the search of the species of interest while the good health of the researchers is preserved. Objective: To identify in molecular, biochemical and morphologically ways some human pathogenic microorganisms in cellulolytic strains of industrial importance stored in the strain bank of the Research Laboratory of the Faculty of Chemical Engineering at the Central University of Ecuador, from Yasuní, Antarctic and Balzapamba. Methods: IA bioprospecting study of cellulolytic bacteria was performed using environmental microbiology techniques. Morphological characteristics were assessed by Gram staining. In addition, biochemical tests and antibiograms were performed for Gram-negative and Gram-positive bacteria. The molecular tests used extraction of bacterial ADN for 16S gene Sanger sequencing. Results: The species Klebsiella pneumoniae (Y2 and Y3r) and Nocardia asteroides (Y1 and Y3p) were found in samples of lignocellulosic material collected in Yasuni, while the isolated species in Antarctica and Balzapamba correspond to Bacillus subtillis. Conclusions: Strains belonging to different bacterial genera were identified. The bacteria of the genus Klebsiella from the samples collected in Yasuní could have a potential pathogen. This can be corroborated with genotyping techniques. Therefore, there could be a risk to humans who perform bioprospecting in that ecosystem and biosecurity measures should be taken. Introducción: La bioprospección de metabolitos de interés antropogénico emplea métodos de recolección de microorganismos en ecosistemas extremófilos o endémicos. La microbiota aislada en estos lugares puede o no incluir microorganismos patógenos. Es imprescindible un enfoque interdisciplinario que permita abordar ... |
format |
Article in Journal/Newspaper |
author |
Salvador Pinos, Carmen Herrera Naranjo, FNicolás Michelena Tupiza, Stephanie González Gavilanez, Humberto Gualoto Oñate, Miguel González Suárez, Erenio Mesa Garriga, Leyanis Rojas Carrillo, Maira |
author_facet |
Salvador Pinos, Carmen Herrera Naranjo, FNicolás Michelena Tupiza, Stephanie González Gavilanez, Humberto Gualoto Oñate, Miguel González Suárez, Erenio Mesa Garriga, Leyanis Rojas Carrillo, Maira |
author_sort |
Salvador Pinos, Carmen |
title |
Polyphasic identification methods applied in searching for microorganisms with industrial potential |
title_short |
Polyphasic identification methods applied in searching for microorganisms with industrial potential |
title_full |
Polyphasic identification methods applied in searching for microorganisms with industrial potential |
title_fullStr |
Polyphasic identification methods applied in searching for microorganisms with industrial potential |
title_full_unstemmed |
Polyphasic identification methods applied in searching for microorganisms with industrial potential |
title_sort |
polyphasic identification methods applied in searching for microorganisms with industrial potential |
publisher |
Universidad Central del Ecuador |
publishDate |
2020 |
url |
https://revistadigital.uce.edu.ec/index.php/CIENCIAS_MEDICAS/article/view/2687 https://doi.org/10.29166/rfcmq.v44i2.2687 |
genre |
Antarc* Antarctic Antarctica Antártida |
genre_facet |
Antarc* Antarctic Antarctica Antártida |
op_source |
Revista de la Facultad de Ciencias Médicas (Quito); Vol. 44 Núm. 2 (2019): Revista de la Facultad de Ciencias Médicas (Quito); 10 - 23 2737-6141 2588-0691 |
op_relation |
https://revistadigital.uce.edu.ec/index.php/CIENCIAS_MEDICAS/article/view/2687/3101 https://revistadigital.uce.edu.ec/index.php/CIENCIAS_MEDICAS/article/view/2687 doi:10.29166/rfcmq.v44i2.2687 |
op_rights |
Derechos de autor 2020 Carmen Salvador Pinos, FNicolás Herrera Naranjo, Stephanie Michelena Tupiza, Humberto González Gavilanez, Miguel Gualoto Oñate, Erenio González Suárez, Leyanis Mesa Garriga, Maira Rojas Carrillo http://creativecommons.org/licenses/by-nc-nd/4.0 |
op_doi |
https://doi.org/10.29166/rfcmq.v44i2.2687 |
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Revista de la Facultad de Ciencias Médicas (Quito) |
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44 |
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10 |
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