Evaluation of the antibacterial activity of microalgae extracts using ATCC strains

• Published in: Química Central
• Main Authors: JENNIFER, SUASNAVAS, RONNY, FLORES, ECHEVERRÍA, INÉS
• Format: Article in Journal/Newspaper
• Language: Spanish
• Published: Universidad Central del Ecuador 2018
• Subjects: Extractos de microalgas; método de Kirby Bauer; halos de inhibición; actividad antibacteriana; microalgae´s extracts; Kirby Bauer method; zones of inhibition; antibacterial activity; Antarc*; Antarctic
• Online Access: https://revistadigital.uce.edu.ec/index.php/QUIMICA/article/view/1416; https://doi.org/10.29166/quimica.v6i1.1416

The presence of multiresistant bacteria is a public health problem worldwide which generates the need to searchtherapeutic alternatives of natural origin. The objective of this research was to evaluate the antibacterial activityof ethanolic, methanolic and hexane extracts of microalgae gender: Chlorella sp., Anabaena sp., Oscillatoriasp. and Stichococcus sp., collected at the Ecuadorian Antarctic Station Pedro Vicente Maldonado. The extractswere obtained by the Soxhlet extraction technique. The antibacterial activity was first evaluated with the agar dilutionmethod, and it was identified that from the 12 extracts evaluated, the methanolic extracts of the microalgaeOscillatoria sp. and Stichococcus sp. presented inhibitory activity against the bacterial strain Staphylococcus aureusATCC 25923, whereas no extract presented activity against Escherichia coli ATCC 25922. With the methanolicextracts of Oscillatoria sp. and Stichococcus sp. the Kirby Bauer method was developed, obtaining zonesof inhibition that shows greater antibacterial activity by the Oscillatoria sp. extract. When comparing the activityof the microalgae extracts with the broad spectrum antibiotics trimethoprim sulfamethoxazole and ciprofloxacin,the conclusion is that these pharmaceutical products cause greater sensitivity. Through phytochemical screeningterpenoids, phenols and alkaloids were identified which could be responsible for antibacterial activityl activity. La presencia de bacterias multirresistentes es un problema de salud pública a nivel mundial, lo que genera lanecesidad de buscar alternativas terapéuticas de preferencia de origen natural. El objetivo del trabajo de investigaciónfue evaluar la actividad antibacteriana de extractos etanólicos, metanólicos y hexánicos de los génerosde microalgas: Chlorella sp., Anabaena sp., Oscillatoria sp. y Stichococcus sp., recolectadas de la estación antárticaecuatoriana Pedro Vicente Maldonado. Los extractos fueron obtenidos mediante la técnica de extracciónSoxhlet. La actividad antibacteriana se ...