Antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near Palmer Station, Antarctica
Antibiotic resistance in aquatic bacteria has increased steadily as a consequence of the widespread use of antibiotics, but practice and international treaty should have limited antibiotic contamination in Antarctica. We estimated antibiotic resistance in microorganisms isolated from the Antarctic m...
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ftunivcardiff:oai:https://orca.cardiff.ac.uk:8758 2023-05-15T13:37:52+02:00 Antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near Palmer Station, Antarctica Miller, R. V. Gammon, K. Day, Martin John 2009-01 https://orca.cardiff.ac.uk/id/eprint/8758/ https://doi.org/10.1139/W08-119 unknown Canadian Science Publishing Miller, R. V., Gammon, K. and Day, Martin John 2009. Antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near Palmer Station, Antarctica. Canadian Journal of Microbiology 55 (1) , pp. 37-45. 10.1139/W08-119 https://doi.org/10.1139/W08-119 doi:10.1139/W08-119 QH301 Biology Article PeerReviewed 2009 ftunivcardiff https://doi.org/10.1139/W08-119 2022-09-25T20:16:50Z Antibiotic resistance in aquatic bacteria has increased steadily as a consequence of the widespread use of antibiotics, but practice and international treaty should have limited antibiotic contamination in Antarctica. We estimated antibiotic resistance in microorganisms isolated from the Antarctic marine waters and a penguin rookery, for 2 reasons: (i) as a measure of human impact and (ii) as a potential “snapshot” of the preantibiotic world. Samples were taken at 4 established sampling sites near Palmer Station, which is situated at the southern end of the Palmer Archipelago (64°10′S, 61°50′W). Sites were chosen to provide different potentials for human contamination. Forty 50 mL samples of seawater were collected and colony-forming units (CFU)/mL were determined at 6 and 20 °C. For this study, presumed psychrophiles (growth at 6 °C) were assumed to be native to Antarctic waters, whereas presumed mesophiles (growth at 20 °C but not at 6 °C) were taken to represent introduced organisms. The 20–6 °C CFU/mL ratio was used as a measure of the relative impact to the ecosystem of presumably introduced organisms. This ratio was highest at the site nearest to Palmer Station and decreased with distance from it, suggesting that human presence has impacted the natural microbial flora of the site. The frequency of resistance to 5 common antibiotics was determined in each group of isolates. Overall drug resistance was higher among the presumed mesophiles than the presumed psychrophiles and increased with proximity to Palmer Station, with the presumed mesophiles showing higher frequencies of single and multiple drug resistance than the psychrophile population. The frequency of multidrug resistance followed the same pattern. It appears that multidrug resistance is low among native Antarctic bacteria but is increased by human habitation. Article in Journal/Newspaper Antarc* Antarctic Antarctica Palmer Archipelago Cardiff University: ORCA (Online Research @ Cardiff) Antarctic Palmer Archipelago ENVELOPE(-62.833,-62.833,-64.250,-64.250) Palmer Station ENVELOPE(-64.050,-64.050,-64.770,-64.770) Palmer-Station ENVELOPE(-64.050,-64.050,-64.770,-64.770) The Antarctic Canadian Journal of Microbiology 55 1 37 45 |
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Cardiff University: ORCA (Online Research @ Cardiff) |
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ftunivcardiff |
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unknown |
topic |
QH301 Biology |
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QH301 Biology Miller, R. V. Gammon, K. Day, Martin John Antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near Palmer Station, Antarctica |
topic_facet |
QH301 Biology |
description |
Antibiotic resistance in aquatic bacteria has increased steadily as a consequence of the widespread use of antibiotics, but practice and international treaty should have limited antibiotic contamination in Antarctica. We estimated antibiotic resistance in microorganisms isolated from the Antarctic marine waters and a penguin rookery, for 2 reasons: (i) as a measure of human impact and (ii) as a potential “snapshot” of the preantibiotic world. Samples were taken at 4 established sampling sites near Palmer Station, which is situated at the southern end of the Palmer Archipelago (64°10′S, 61°50′W). Sites were chosen to provide different potentials for human contamination. Forty 50 mL samples of seawater were collected and colony-forming units (CFU)/mL were determined at 6 and 20 °C. For this study, presumed psychrophiles (growth at 6 °C) were assumed to be native to Antarctic waters, whereas presumed mesophiles (growth at 20 °C but not at 6 °C) were taken to represent introduced organisms. The 20–6 °C CFU/mL ratio was used as a measure of the relative impact to the ecosystem of presumably introduced organisms. This ratio was highest at the site nearest to Palmer Station and decreased with distance from it, suggesting that human presence has impacted the natural microbial flora of the site. The frequency of resistance to 5 common antibiotics was determined in each group of isolates. Overall drug resistance was higher among the presumed mesophiles than the presumed psychrophiles and increased with proximity to Palmer Station, with the presumed mesophiles showing higher frequencies of single and multiple drug resistance than the psychrophile population. The frequency of multidrug resistance followed the same pattern. It appears that multidrug resistance is low among native Antarctic bacteria but is increased by human habitation. |
format |
Article in Journal/Newspaper |
author |
Miller, R. V. Gammon, K. Day, Martin John |
author_facet |
Miller, R. V. Gammon, K. Day, Martin John |
author_sort |
Miller, R. V. |
title |
Antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near Palmer Station, Antarctica |
title_short |
Antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near Palmer Station, Antarctica |
title_full |
Antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near Palmer Station, Antarctica |
title_fullStr |
Antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near Palmer Station, Antarctica |
title_full_unstemmed |
Antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near Palmer Station, Antarctica |
title_sort |
antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near palmer station, antarctica |
publisher |
Canadian Science Publishing |
publishDate |
2009 |
url |
https://orca.cardiff.ac.uk/id/eprint/8758/ https://doi.org/10.1139/W08-119 |
long_lat |
ENVELOPE(-62.833,-62.833,-64.250,-64.250) ENVELOPE(-64.050,-64.050,-64.770,-64.770) ENVELOPE(-64.050,-64.050,-64.770,-64.770) |
geographic |
Antarctic Palmer Archipelago Palmer Station Palmer-Station The Antarctic |
geographic_facet |
Antarctic Palmer Archipelago Palmer Station Palmer-Station The Antarctic |
genre |
Antarc* Antarctic Antarctica Palmer Archipelago |
genre_facet |
Antarc* Antarctic Antarctica Palmer Archipelago |
op_relation |
Miller, R. V., Gammon, K. and Day, Martin John 2009. Antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near Palmer Station, Antarctica. Canadian Journal of Microbiology 55 (1) , pp. 37-45. 10.1139/W08-119 https://doi.org/10.1139/W08-119 doi:10.1139/W08-119 |
op_doi |
https://doi.org/10.1139/W08-119 |
container_title |
Canadian Journal of Microbiology |
container_volume |
55 |
container_issue |
1 |
container_start_page |
37 |
op_container_end_page |
45 |
_version_ |
1766098517049212928 |