Development and multiplex PCR amplification of novel microsatellite markers in the White-tailed Sea Eagle, Haliaeetus albicilla (Aves : Falconiformes, Accipitridae)
We report the development of 14 novel polymorphic microsatellite markers cloned from the White-tailed Sea Eagle, Haliaeetus albicilla, a formerly threatened raptor that has received much conservation attention throughout Eurasia. We also present a protocol for multiplex polymerase chain reaction (PC...
Published in: | Molecular Ecology Notes |
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2005
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ftunivcardiff:oai:https://orca.cardiff.ac.uk:69937 2023-05-15T16:32:39+02:00 Development and multiplex PCR amplification of novel microsatellite markers in the White-tailed Sea Eagle, Haliaeetus albicilla (Aves : Falconiformes, Accipitridae) Hailer, Frank Gautschi, B. Helander, B. 2005-12 https://orca.cardiff.ac.uk/id/eprint/69937/ https://doi.org/10.1111/j.1471-8286.2005.01122.x unknown Wiley-Blackwell Hailer, Frank https://orca.cardiff.ac.uk/view/cardiffauthors/A21394518.html orcid:0000-0002-2340-1726 orcid:0000-0002-2340-1726, Gautschi, B. and Helander, B. 2005. Development and multiplex PCR amplification of novel microsatellite markers in the White-tailed Sea Eagle, Haliaeetus albicilla (Aves : Falconiformes, Accipitridae). Molecular Ecology Notes 5 (4) , pp. 938-940. 10.1111/j.1471-8286.2005.01122.x https://doi.org/10.1111/j.1471-8286.2005.01122.x doi:10.1111/j.1471-8286.2005.01122.x Article PeerReviewed 2005 ftunivcardiff https://doi.org/10.1111/j.1471-8286.2005.01122.x 2022-10-27T22:41:49Z We report the development of 14 novel polymorphic microsatellite markers cloned from the White-tailed Sea Eagle, Haliaeetus albicilla, a formerly threatened raptor that has received much conservation attention throughout Eurasia. We also present a protocol for multiplex polymerase chain reaction (PCR) amplification of the loci. Among 40 unrelated H. albicilla individuals from southern Sweden, the markers produced two to eight alleles per locus, and average observed and expected heterozygosities were 0.463 and 0.468, respectively. We further present five microsatellite markers that appeared monomorphic in H. albicilla, but which may be of interest for use in other raptor species. Article in Journal/Newspaper Haliaeetus albicilla Cardiff University: ORCA (Online Research @ Cardiff) Molecular Ecology Notes 5 4 938 940 |
institution |
Open Polar |
collection |
Cardiff University: ORCA (Online Research @ Cardiff) |
op_collection_id |
ftunivcardiff |
language |
unknown |
description |
We report the development of 14 novel polymorphic microsatellite markers cloned from the White-tailed Sea Eagle, Haliaeetus albicilla, a formerly threatened raptor that has received much conservation attention throughout Eurasia. We also present a protocol for multiplex polymerase chain reaction (PCR) amplification of the loci. Among 40 unrelated H. albicilla individuals from southern Sweden, the markers produced two to eight alleles per locus, and average observed and expected heterozygosities were 0.463 and 0.468, respectively. We further present five microsatellite markers that appeared monomorphic in H. albicilla, but which may be of interest for use in other raptor species. |
format |
Article in Journal/Newspaper |
author |
Hailer, Frank Gautschi, B. Helander, B. |
spellingShingle |
Hailer, Frank Gautschi, B. Helander, B. Development and multiplex PCR amplification of novel microsatellite markers in the White-tailed Sea Eagle, Haliaeetus albicilla (Aves : Falconiformes, Accipitridae) |
author_facet |
Hailer, Frank Gautschi, B. Helander, B. |
author_sort |
Hailer, Frank |
title |
Development and multiplex PCR amplification of novel microsatellite markers in the White-tailed Sea Eagle, Haliaeetus albicilla (Aves : Falconiformes, Accipitridae) |
title_short |
Development and multiplex PCR amplification of novel microsatellite markers in the White-tailed Sea Eagle, Haliaeetus albicilla (Aves : Falconiformes, Accipitridae) |
title_full |
Development and multiplex PCR amplification of novel microsatellite markers in the White-tailed Sea Eagle, Haliaeetus albicilla (Aves : Falconiformes, Accipitridae) |
title_fullStr |
Development and multiplex PCR amplification of novel microsatellite markers in the White-tailed Sea Eagle, Haliaeetus albicilla (Aves : Falconiformes, Accipitridae) |
title_full_unstemmed |
Development and multiplex PCR amplification of novel microsatellite markers in the White-tailed Sea Eagle, Haliaeetus albicilla (Aves : Falconiformes, Accipitridae) |
title_sort |
development and multiplex pcr amplification of novel microsatellite markers in the white-tailed sea eagle, haliaeetus albicilla (aves : falconiformes, accipitridae) |
publisher |
Wiley-Blackwell |
publishDate |
2005 |
url |
https://orca.cardiff.ac.uk/id/eprint/69937/ https://doi.org/10.1111/j.1471-8286.2005.01122.x |
genre |
Haliaeetus albicilla |
genre_facet |
Haliaeetus albicilla |
op_relation |
Hailer, Frank https://orca.cardiff.ac.uk/view/cardiffauthors/A21394518.html orcid:0000-0002-2340-1726 orcid:0000-0002-2340-1726, Gautschi, B. and Helander, B. 2005. Development and multiplex PCR amplification of novel microsatellite markers in the White-tailed Sea Eagle, Haliaeetus albicilla (Aves : Falconiformes, Accipitridae). Molecular Ecology Notes 5 (4) , pp. 938-940. 10.1111/j.1471-8286.2005.01122.x https://doi.org/10.1111/j.1471-8286.2005.01122.x doi:10.1111/j.1471-8286.2005.01122.x |
op_doi |
https://doi.org/10.1111/j.1471-8286.2005.01122.x |
container_title |
Molecular Ecology Notes |
container_volume |
5 |
container_issue |
4 |
container_start_page |
938 |
op_container_end_page |
940 |
_version_ |
1766022411061297152 |