Identification and evaluation of novel di- and tetranucleotide microsatellite markers from the brown bear (Ursus arctos)
We here describe 34 novel microsatellite loci from the brown bear (Ursus arctos) identified using a next-generation sequencing approach. While we found allelic variation in 30 of these, we selected 16 polymorphic microsatellites (11 di- and 5 tetranucleotide repeats) for four multiplex-PCR assays ba...
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Springer Verlag
2012
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Online Access: | https://orca.cardiff.ac.uk/id/eprint/69927/ https://doi.org/10.1007/s12686-012-9634-5 |
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ftunivcardiff:oai:https://orca.cardiff.ac.uk:69927 2023-05-15T18:41:48+02:00 Identification and evaluation of novel di- and tetranucleotide microsatellite markers from the brown bear (Ursus arctos) Kleven, Oddmund Hallstroem, Bjoern M. Hailer, Frank Janke, Axel Hagen, Snorre B. Kopatz, Alexander Eiken, Hans Geir 2012-09-01 https://orca.cardiff.ac.uk/id/eprint/69927/ https://doi.org/10.1007/s12686-012-9634-5 unknown Springer Verlag Kleven, Oddmund, Hallstroem, Bjoern M., Hailer, Frank https://orca.cardiff.ac.uk/view/cardiffauthors/A21394518.html orcid:0000-0002-2340-1726 orcid:0000-0002-2340-1726, Janke, Axel, Hagen, Snorre B., Kopatz, Alexander and Eiken, Hans Geir 2012. Identification and evaluation of novel di- and tetranucleotide microsatellite markers from the brown bear (Ursus arctos). Conservation Genetics Resources 4 (3) , pp. 737-741. 10.1007/s12686-012-9634-5 https://doi.org/10.1007/s12686-012-9634-5 doi:10.1007/s12686-012-9634-5 Article PeerReviewed 2012 ftunivcardiff https://doi.org/10.1007/s12686-012-9634-5 2022-10-27T22:41:49Z We here describe 34 novel microsatellite loci from the brown bear (Ursus arctos) identified using a next-generation sequencing approach. While we found allelic variation in 30 of these, we selected 16 polymorphic microsatellites (11 di- and 5 tetranucleotide repeats) for four multiplex-PCR assays based on genetic variability, PCR performance and interpretability. Applying these assays for the 16 novel microsatellites, we screened 31 brown bears from a northern European population. The number of alleles encountered at single loci was between 4 and 10, and expected and observed heterozygosities ranged between 0.66–0.85 and 0.60–0.97, respectively. Seven of 120 pairwise locus combinations showed linkage disequilibrium and one locus deviated from Hardy–Weinberg equilibrium, while no null-alleles were detected. Our novel multiplex-PCR assays may be useful in forensics, parentage and population genetic studies. Article in Journal/Newspaper Ursus arctos Cardiff University: ORCA (Online Research @ Cardiff) Conservation Genetics Resources 4 3 737 741 |
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Open Polar |
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Cardiff University: ORCA (Online Research @ Cardiff) |
op_collection_id |
ftunivcardiff |
language |
unknown |
description |
We here describe 34 novel microsatellite loci from the brown bear (Ursus arctos) identified using a next-generation sequencing approach. While we found allelic variation in 30 of these, we selected 16 polymorphic microsatellites (11 di- and 5 tetranucleotide repeats) for four multiplex-PCR assays based on genetic variability, PCR performance and interpretability. Applying these assays for the 16 novel microsatellites, we screened 31 brown bears from a northern European population. The number of alleles encountered at single loci was between 4 and 10, and expected and observed heterozygosities ranged between 0.66–0.85 and 0.60–0.97, respectively. Seven of 120 pairwise locus combinations showed linkage disequilibrium and one locus deviated from Hardy–Weinberg equilibrium, while no null-alleles were detected. Our novel multiplex-PCR assays may be useful in forensics, parentage and population genetic studies. |
format |
Article in Journal/Newspaper |
author |
Kleven, Oddmund Hallstroem, Bjoern M. Hailer, Frank Janke, Axel Hagen, Snorre B. Kopatz, Alexander Eiken, Hans Geir |
spellingShingle |
Kleven, Oddmund Hallstroem, Bjoern M. Hailer, Frank Janke, Axel Hagen, Snorre B. Kopatz, Alexander Eiken, Hans Geir Identification and evaluation of novel di- and tetranucleotide microsatellite markers from the brown bear (Ursus arctos) |
author_facet |
Kleven, Oddmund Hallstroem, Bjoern M. Hailer, Frank Janke, Axel Hagen, Snorre B. Kopatz, Alexander Eiken, Hans Geir |
author_sort |
Kleven, Oddmund |
title |
Identification and evaluation of novel di- and tetranucleotide microsatellite markers from the brown bear (Ursus arctos) |
title_short |
Identification and evaluation of novel di- and tetranucleotide microsatellite markers from the brown bear (Ursus arctos) |
title_full |
Identification and evaluation of novel di- and tetranucleotide microsatellite markers from the brown bear (Ursus arctos) |
title_fullStr |
Identification and evaluation of novel di- and tetranucleotide microsatellite markers from the brown bear (Ursus arctos) |
title_full_unstemmed |
Identification and evaluation of novel di- and tetranucleotide microsatellite markers from the brown bear (Ursus arctos) |
title_sort |
identification and evaluation of novel di- and tetranucleotide microsatellite markers from the brown bear (ursus arctos) |
publisher |
Springer Verlag |
publishDate |
2012 |
url |
https://orca.cardiff.ac.uk/id/eprint/69927/ https://doi.org/10.1007/s12686-012-9634-5 |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_relation |
Kleven, Oddmund, Hallstroem, Bjoern M., Hailer, Frank https://orca.cardiff.ac.uk/view/cardiffauthors/A21394518.html orcid:0000-0002-2340-1726 orcid:0000-0002-2340-1726, Janke, Axel, Hagen, Snorre B., Kopatz, Alexander and Eiken, Hans Geir 2012. Identification and evaluation of novel di- and tetranucleotide microsatellite markers from the brown bear (Ursus arctos). Conservation Genetics Resources 4 (3) , pp. 737-741. 10.1007/s12686-012-9634-5 https://doi.org/10.1007/s12686-012-9634-5 doi:10.1007/s12686-012-9634-5 |
op_doi |
https://doi.org/10.1007/s12686-012-9634-5 |
container_title |
Conservation Genetics Resources |
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4 |
container_issue |
3 |
container_start_page |
737 |
op_container_end_page |
741 |
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1766231348393017344 |