Cloning and unusual expression profile of the aldolase B gene from Atlantic salmon

• Published in: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression
• Main Authors: Llewellyn, Lynda, Sweeney, Glen E., Ramsurn, Vimi P., Rogers, Sarah A., Wigham, Trevor
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Elsevier 1998
• Subjects: Atlantic salmon; Salmo salar
• Online Access: https://orca.cardiff.ac.uk/id/eprint/65593/; https://doi.org/10.1016/S0167-4781(98)00229-2

A full-length clone of the aldolase B gene has been isolated from a cDNA library constructed from liver of Atlantic salmon (Salmo salar). Sequencing showed that the clone encodes a typical aldolase B, possessing a number of amino acid residues which are seen in aldolase B, but not in other aldolase isoforms. RT-PCR analysis showed that the gene is expressed in liver, kidney and intestine as expected. However, in contrast to mammalian and avian aldolase B, expression was also found in a number of other tissues. Levels of aldolase B mRNA in liver and kidney were not significantly altered during smoltification, the transformation of freshwater-dwelling salmon (parr) into saltwater-adapted salmon (smolts).