Stability of RNA isolated from post-mortem tissues of Atlantic salmon (Salmo salar L.)
Studies of post-mortem interval on the stability of RNA from a number of various mammals have shown RNA to be stable for between 24 and 48 h following death. As yet there have been no studies looking at RNA stability in post-mortem tissues of poikilothermic fish. Brain, kidney, liver and muscle were...
Published in: | Fish Physiology and Biochemistry |
---|---|
Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Springer
2007
|
Subjects: | |
Online Access: | https://orca.cardiff.ac.uk/id/eprint/65574/ https://doi.org/10.1007/s10695-007-9141-x |
id |
ftunivcardiff:oai:https://orca.cardiff.ac.uk:65574 |
---|---|
record_format |
openpolar |
spelling |
ftunivcardiff:oai:https://orca.cardiff.ac.uk:65574 2023-05-15T15:31:05+02:00 Stability of RNA isolated from post-mortem tissues of Atlantic salmon (Salmo salar L.) Seear, P. J. Sweeney, Glen E. 2007 https://orca.cardiff.ac.uk/id/eprint/65574/ https://doi.org/10.1007/s10695-007-9141-x unknown Springer Seear, P. J. and Sweeney, Glen E. https://orca.cardiff.ac.uk/view/cardiffauthors/A032676H.html 2007. Stability of RNA isolated from post-mortem tissues of Atlantic salmon (Salmo salar L.). Fish Physiology and Biochemistry 34 (1) , pp. 19-24. 10.1007/s10695-007-9141-x https://doi.org/10.1007/s10695-007-9141-x doi:10.1007/s10695-007-9141-x Article PeerReviewed 2007 ftunivcardiff https://doi.org/10.1007/s10695-007-9141-x 2022-09-25T20:43:37Z Studies of post-mortem interval on the stability of RNA from a number of various mammals have shown RNA to be stable for between 24 and 48 h following death. As yet there have been no studies looking at RNA stability in post-mortem tissues of poikilothermic fish. Brain, kidney, liver and muscle were collected from Atlantic salmon (Salmo salar) parr and samples of each tissue were placed into RNAlater™ after 0–24 h post-mortem storage at room temperature. Electrophoretic analysis of the total RNA showed degradation of ribosomal RNA only in muscle from 8 h onwards. Probing of northern blots with β-actin showed that, in the brain, β-actin mRNA was stable for 24 h post-mortem but degradation of mRNA was observed after 8 h with the kidney and liver and after 4 h with the muscle. Expression of the weakly expressed thyroid hormone receptor β (TRβ) was detected by reverse transcriptase polymerase chain reaction (RT-PCR) in all tissues up to 24 h post-mortem although a reduction in PCR product was observed after 8 h with muscle and 24 h with kidney. Analysis with an Agilent 2100 Bioanalyzer showed that the RNA integrity number (RIN) of brain total RNA remained constant for 8 h post-mortem with only a small fall at 24 h post-mortem. The RINs of the remaining tissues indicated degradation at 8 h post-mortem with kidney and muscle and at 24 hours post-mortem with liver. Taken together these findings show that degradation of Atlantic salmon RNA is tissue dependent but stable for at least one hour post-mortem. Article in Journal/Newspaper Atlantic salmon Salmo salar Cardiff University: ORCA (Online Research @ Cardiff) Fish Physiology and Biochemistry 34 1 19 24 |
institution |
Open Polar |
collection |
Cardiff University: ORCA (Online Research @ Cardiff) |
op_collection_id |
ftunivcardiff |
language |
unknown |
description |
Studies of post-mortem interval on the stability of RNA from a number of various mammals have shown RNA to be stable for between 24 and 48 h following death. As yet there have been no studies looking at RNA stability in post-mortem tissues of poikilothermic fish. Brain, kidney, liver and muscle were collected from Atlantic salmon (Salmo salar) parr and samples of each tissue were placed into RNAlater™ after 0–24 h post-mortem storage at room temperature. Electrophoretic analysis of the total RNA showed degradation of ribosomal RNA only in muscle from 8 h onwards. Probing of northern blots with β-actin showed that, in the brain, β-actin mRNA was stable for 24 h post-mortem but degradation of mRNA was observed after 8 h with the kidney and liver and after 4 h with the muscle. Expression of the weakly expressed thyroid hormone receptor β (TRβ) was detected by reverse transcriptase polymerase chain reaction (RT-PCR) in all tissues up to 24 h post-mortem although a reduction in PCR product was observed after 8 h with muscle and 24 h with kidney. Analysis with an Agilent 2100 Bioanalyzer showed that the RNA integrity number (RIN) of brain total RNA remained constant for 8 h post-mortem with only a small fall at 24 h post-mortem. The RINs of the remaining tissues indicated degradation at 8 h post-mortem with kidney and muscle and at 24 hours post-mortem with liver. Taken together these findings show that degradation of Atlantic salmon RNA is tissue dependent but stable for at least one hour post-mortem. |
format |
Article in Journal/Newspaper |
author |
Seear, P. J. Sweeney, Glen E. |
spellingShingle |
Seear, P. J. Sweeney, Glen E. Stability of RNA isolated from post-mortem tissues of Atlantic salmon (Salmo salar L.) |
author_facet |
Seear, P. J. Sweeney, Glen E. |
author_sort |
Seear, P. J. |
title |
Stability of RNA isolated from post-mortem tissues of Atlantic salmon (Salmo salar L.) |
title_short |
Stability of RNA isolated from post-mortem tissues of Atlantic salmon (Salmo salar L.) |
title_full |
Stability of RNA isolated from post-mortem tissues of Atlantic salmon (Salmo salar L.) |
title_fullStr |
Stability of RNA isolated from post-mortem tissues of Atlantic salmon (Salmo salar L.) |
title_full_unstemmed |
Stability of RNA isolated from post-mortem tissues of Atlantic salmon (Salmo salar L.) |
title_sort |
stability of rna isolated from post-mortem tissues of atlantic salmon (salmo salar l.) |
publisher |
Springer |
publishDate |
2007 |
url |
https://orca.cardiff.ac.uk/id/eprint/65574/ https://doi.org/10.1007/s10695-007-9141-x |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Seear, P. J. and Sweeney, Glen E. https://orca.cardiff.ac.uk/view/cardiffauthors/A032676H.html 2007. Stability of RNA isolated from post-mortem tissues of Atlantic salmon (Salmo salar L.). Fish Physiology and Biochemistry 34 (1) , pp. 19-24. 10.1007/s10695-007-9141-x https://doi.org/10.1007/s10695-007-9141-x doi:10.1007/s10695-007-9141-x |
op_doi |
https://doi.org/10.1007/s10695-007-9141-x |
container_title |
Fish Physiology and Biochemistry |
container_volume |
34 |
container_issue |
1 |
container_start_page |
19 |
op_container_end_page |
24 |
_version_ |
1766361571781509120 |