Isolation of viral haemorrhagic septicaemia virus from Atlantic herring Clupea harengus from the Atlantic herring Clupea harengus from the English channel

• Main Authors: Dixon, P.F., Feist, S., Kehoe, E., Parry, Lee, Stone, D.M., Way, K.
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Inter Research 1997
• Subjects: QH301 Biology; QR355 Virology; Gadus morhua
• Online Access: https://orca.cardiff.ac.uk/id/eprint/47549/

Viral haemorrhaglc septicaemia vuus (VHSV) was isolated from apparently healthy Atlantlc herrlng Clupea harengus from the English Channel The virus was isolated in bluegill fibroblast (BF-2) cells but not epithelioma papulosum cypnni (EPC) cells, however, the virus was passaged from BF-2 to EPC cells The identlty of the virus was confirmed by an enzyme-llnked ~rnmunosorbent assay and by a reverse transcnptase-polymerase chain reaction (RT-PCR) The virus was classified as a member of genogroup 111 of VHSV which comprises many European freshwater and manne VHSV 150- lates Sequence comparisons of the RT-PCR products showed that the herring isolate was closely related (99 1 % nucleotlde similarity) to a VHSV isolate from Atlantlc cod Gadus morhua both of those isolates were avirulent for rainbow trout Oncorhynchus myklss by bath infection Circumstantial evidence is presented which suggests that VHSV from herring could have been the source of VHS disease in rainbow trout in Denmark