Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species
Infectious diseases represent an important barrier to sustainable aquaculture development. Rearing density can substantially impact fish productivity, health and welfare in aquaculture, including growth rates, behaviour and, crucially, immune activity. Given the current emphasis on aquaculture diver...
Published in: | Fish & Shellfish Immunology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Elsevier
2020
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Online Access: | https://orca.cardiff.ac.uk/id/eprint/131820/ https://doi.org/10.1016/j.fsi.2020.05.043 https://orca.cardiff.ac.uk/id/eprint/131820/1/salmon_stocking_FSI_revision_final.pdf |
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ftunivcardiff:oai:https://orca.cardiff.ac.uk:131820 2023-05-15T15:32:34+02:00 Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species Ellison, Amy R. Uren Webster, Tamsyn M. Rodriguez-Barreto, Deiene Garcia de Leaniz, Carlos Consuegra, Sofia Orozco-terWengel, Pablo Cable, Jo 2020-09-30 application/pdf https://orca.cardiff.ac.uk/id/eprint/131820/ https://doi.org/10.1016/j.fsi.2020.05.043 https://orca.cardiff.ac.uk/id/eprint/131820/1/salmon_stocking_FSI_revision_final.pdf en eng Elsevier https://orca.cardiff.ac.uk/id/eprint/131820/1/salmon_stocking_FSI_revision_final.pdf Ellison, Amy R. https://orca.cardiff.ac.uk/view/cardiffauthors/A2177013U.html, Uren Webster, Tamsyn M., Rodriguez-Barreto, Deiene, Garcia de Leaniz, Carlos, Consuegra, Sofia, Orozco-terWengel, Pablo https://orca.cardiff.ac.uk/view/cardiffauthors/A337280B.html orcid:0000-0002-7951-4148 orcid:0000-0002-7951-4148 and Cable, Jo https://orca.cardiff.ac.uk/view/cardiffauthors/A042952A.html orcid:0000-0002-8510-7055 orcid:0000-0002-8510-7055 2020. Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species. Fish and Shellfish Immunology 104 , pp. 192-201. 10.1016/j.fsi.2020.05.043 https://doi.org/10.1016/j.fsi.2020.05.043 file https://orca.cardiff.ac.uk/id/eprint/131820/1/salmon_stocking_FSI_revision_final.pdf doi:10.1016/j.fsi.2020.05.043 cc_by_nc_nd CC-BY-NC-ND Article PeerReviewed 2020 ftunivcardiff https://doi.org/10.1016/j.fsi.2020.05.043 2022-11-10T23:36:51Z Infectious diseases represent an important barrier to sustainable aquaculture development. Rearing density can substantially impact fish productivity, health and welfare in aquaculture, including growth rates, behaviour and, crucially, immune activity. Given the current emphasis on aquaculture diversification, stress-related indicators broadly applicable across species are needed. Utilising an interspecific comparative transcriptomic (RNAseq) approach, we compared gill gene expression responses of Atlantic salmon (Salmo salar) and Nile tilapia (Oreochromis niloticus) to rearing density and Saprolegnia parasitica infection. Salmon reared at high-density showed increased expression of stress-related markers (e.g. c-fos and hsp70), and downregulation of innate immune genes. Upon pathogen challenge, only salmon reared at low density exhibited increased expression of inflammatory interleukins and lymphocyte-related genes. Tilapia immunity, in contrast, was impaired at low-density. Using overlapping gene ontology enrichment and gene ortholog analyses, we found that density-related stress similarly impacted salmon and tilapia in key immune pathways, altering the expression of genes vital to inflammatory and Th17 responses to pathogen challenge. Given the challenges posed by ectoparasites and gill diseases in fish farms, this study underscores the importance of optimal rearing densities for immunocompetence, particularly for mucosal immunity. Our comparative transcriptomics analyses identified density stress impacted immune markers common across different fish taxa, providing key molecular targets with potential for monitoring and enhancing aquaculture resilience in a wide range of farmed species. Article in Journal/Newspaper Atlantic salmon Salmo salar Cardiff University: ORCA (Online Research @ Cardiff) Fish & Shellfish Immunology 104 192 201 |
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Open Polar |
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Cardiff University: ORCA (Online Research @ Cardiff) |
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ftunivcardiff |
language |
English |
description |
Infectious diseases represent an important barrier to sustainable aquaculture development. Rearing density can substantially impact fish productivity, health and welfare in aquaculture, including growth rates, behaviour and, crucially, immune activity. Given the current emphasis on aquaculture diversification, stress-related indicators broadly applicable across species are needed. Utilising an interspecific comparative transcriptomic (RNAseq) approach, we compared gill gene expression responses of Atlantic salmon (Salmo salar) and Nile tilapia (Oreochromis niloticus) to rearing density and Saprolegnia parasitica infection. Salmon reared at high-density showed increased expression of stress-related markers (e.g. c-fos and hsp70), and downregulation of innate immune genes. Upon pathogen challenge, only salmon reared at low density exhibited increased expression of inflammatory interleukins and lymphocyte-related genes. Tilapia immunity, in contrast, was impaired at low-density. Using overlapping gene ontology enrichment and gene ortholog analyses, we found that density-related stress similarly impacted salmon and tilapia in key immune pathways, altering the expression of genes vital to inflammatory and Th17 responses to pathogen challenge. Given the challenges posed by ectoparasites and gill diseases in fish farms, this study underscores the importance of optimal rearing densities for immunocompetence, particularly for mucosal immunity. Our comparative transcriptomics analyses identified density stress impacted immune markers common across different fish taxa, providing key molecular targets with potential for monitoring and enhancing aquaculture resilience in a wide range of farmed species. |
format |
Article in Journal/Newspaper |
author |
Ellison, Amy R. Uren Webster, Tamsyn M. Rodriguez-Barreto, Deiene Garcia de Leaniz, Carlos Consuegra, Sofia Orozco-terWengel, Pablo Cable, Jo |
spellingShingle |
Ellison, Amy R. Uren Webster, Tamsyn M. Rodriguez-Barreto, Deiene Garcia de Leaniz, Carlos Consuegra, Sofia Orozco-terWengel, Pablo Cable, Jo Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species |
author_facet |
Ellison, Amy R. Uren Webster, Tamsyn M. Rodriguez-Barreto, Deiene Garcia de Leaniz, Carlos Consuegra, Sofia Orozco-terWengel, Pablo Cable, Jo |
author_sort |
Ellison, Amy R. |
title |
Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species |
title_short |
Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species |
title_full |
Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species |
title_fullStr |
Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species |
title_full_unstemmed |
Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species |
title_sort |
comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species |
publisher |
Elsevier |
publishDate |
2020 |
url |
https://orca.cardiff.ac.uk/id/eprint/131820/ https://doi.org/10.1016/j.fsi.2020.05.043 https://orca.cardiff.ac.uk/id/eprint/131820/1/salmon_stocking_FSI_revision_final.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://orca.cardiff.ac.uk/id/eprint/131820/1/salmon_stocking_FSI_revision_final.pdf Ellison, Amy R. https://orca.cardiff.ac.uk/view/cardiffauthors/A2177013U.html, Uren Webster, Tamsyn M., Rodriguez-Barreto, Deiene, Garcia de Leaniz, Carlos, Consuegra, Sofia, Orozco-terWengel, Pablo https://orca.cardiff.ac.uk/view/cardiffauthors/A337280B.html orcid:0000-0002-7951-4148 orcid:0000-0002-7951-4148 and Cable, Jo https://orca.cardiff.ac.uk/view/cardiffauthors/A042952A.html orcid:0000-0002-8510-7055 orcid:0000-0002-8510-7055 2020. Comparative transcriptomics reveal conserved impacts of rearing density on immune response of two important aquaculture species. Fish and Shellfish Immunology 104 , pp. 192-201. 10.1016/j.fsi.2020.05.043 https://doi.org/10.1016/j.fsi.2020.05.043 file https://orca.cardiff.ac.uk/id/eprint/131820/1/salmon_stocking_FSI_revision_final.pdf doi:10.1016/j.fsi.2020.05.043 |
op_rights |
cc_by_nc_nd |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.fsi.2020.05.043 |
container_title |
Fish & Shellfish Immunology |
container_volume |
104 |
container_start_page |
192 |
op_container_end_page |
201 |
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1766363056420421632 |