Quantification of brown dog tick repellents, 2-hexanone and benzaldehyde, and release from tick-resistant beagles, Canis lupus familiaris
We have recently shown that repellency of the tick Rhipicephalus sanguineus sensu lato by the tick resistant dog breed, the beagle, is mediated by volatile organic compounds (VOCs) 2-hexanone and benzaldehyde present in beagle odour. Ectoparasite location of animal hosts is affected by variation in...
Published in: | Journal of Chromatography B |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Elsevier
2016
|
Subjects: | |
Online Access: | https://orca.cardiff.ac.uk/id/eprint/106848/ https://doi.org/10.1016/j.jchromb.2016.03.014 |
id |
ftunivcardiff:oai:https://orca.cardiff.ac.uk:106848 |
---|---|
record_format |
openpolar |
spelling |
ftunivcardiff:oai:https://orca.cardiff.ac.uk:106848 2023-05-15T15:50:47+02:00 Quantification of brown dog tick repellents, 2-hexanone and benzaldehyde, and release from tick-resistant beagles, Canis lupus familiaris de Oliveira Filho, Jaires Gomes Sarria, André Lucio Franceschini Ferreira, Lorena Lopes Caulfield, John C. Powers, Stephen J. Pickett, John A. de León, Adalberto A. Pérez Birkett, Michael A. Borges, Lígia Miranda Ferreira 2016-06-01 https://orca.cardiff.ac.uk/id/eprint/106848/ https://doi.org/10.1016/j.jchromb.2016.03.014 unknown Elsevier de Oliveira Filho, Jaires Gomes, Sarria, André Lucio Franceschini, Ferreira, Lorena Lopes, Caulfield, John C., Powers, Stephen J., Pickett, John A. https://orca.cardiff.ac.uk/view/cardiffauthors/A23568735.html orcid:0000-0002-1008-6595 orcid:0000-0002-1008-6595, de León, Adalberto A. Pérez, Birkett, Michael A. and Borges, Lígia Miranda Ferreira 2016. Quantification of brown dog tick repellents, 2-hexanone and benzaldehyde, and release from tick-resistant beagles, Canis lupus familiaris. Journal of Chromatography B 1022 , pp. 64-69. 10.1016/j.jchromb.2016.03.014 https://doi.org/10.1016/j.jchromb.2016.03.014 doi:10.1016/j.jchromb.2016.03.014 Article PeerReviewed 2016 ftunivcardiff https://doi.org/10.1016/j.jchromb.2016.03.014 2022-11-03T23:43:58Z We have recently shown that repellency of the tick Rhipicephalus sanguineus sensu lato by the tick resistant dog breed, the beagle, is mediated by volatile organic compounds (VOCs) 2-hexanone and benzaldehyde present in beagle odour. Ectoparasite location of animal hosts is affected by variation in these odour components and their ratios. The aim of this study was to quantify the release rate, and the ratio, of 2-hexanone and benzaldehyde from beagles. The odour of three beagles was collected, for four days, over one week (day 0, day 1, day 4 and day 7). The compounds were identified using coupled high-resolution gas chromatography–mass spectrometry (GC–MS), and authentic standards of compounds were used to generate external calibration curves for quantification. Both compounds were found in all dogs on all days. The amount of benzaldehyde was always higher than that of 2-hexanone and so their ratio varied from unity, on average (over time) being 3.128 ± 0.365, 1.902 ± 0.390, 1.670 ± 0.671 ng mL−1 for beagle 1, 2 and 3, respectively. There was no significant (p < 0.05, F-test) effect of time. The overall mean was 2.233 ± 0.387 ng mL−1. These results further previous findings by documenting the presence of 2-hexanone and benzaldehyde in beagle odour samples covering a 7-day period. This knowledge enables development of repellents to protect dogs from R. sanguineus s. l. infestation. Article in Journal/Newspaper Canis lupus Cardiff University: ORCA (Online Research @ Cardiff) Journal of Chromatography B 1022 64 69 |
institution |
Open Polar |
collection |
Cardiff University: ORCA (Online Research @ Cardiff) |
op_collection_id |
ftunivcardiff |
language |
unknown |
description |
We have recently shown that repellency of the tick Rhipicephalus sanguineus sensu lato by the tick resistant dog breed, the beagle, is mediated by volatile organic compounds (VOCs) 2-hexanone and benzaldehyde present in beagle odour. Ectoparasite location of animal hosts is affected by variation in these odour components and their ratios. The aim of this study was to quantify the release rate, and the ratio, of 2-hexanone and benzaldehyde from beagles. The odour of three beagles was collected, for four days, over one week (day 0, day 1, day 4 and day 7). The compounds were identified using coupled high-resolution gas chromatography–mass spectrometry (GC–MS), and authentic standards of compounds were used to generate external calibration curves for quantification. Both compounds were found in all dogs on all days. The amount of benzaldehyde was always higher than that of 2-hexanone and so their ratio varied from unity, on average (over time) being 3.128 ± 0.365, 1.902 ± 0.390, 1.670 ± 0.671 ng mL−1 for beagle 1, 2 and 3, respectively. There was no significant (p < 0.05, F-test) effect of time. The overall mean was 2.233 ± 0.387 ng mL−1. These results further previous findings by documenting the presence of 2-hexanone and benzaldehyde in beagle odour samples covering a 7-day period. This knowledge enables development of repellents to protect dogs from R. sanguineus s. l. infestation. |
format |
Article in Journal/Newspaper |
author |
de Oliveira Filho, Jaires Gomes Sarria, André Lucio Franceschini Ferreira, Lorena Lopes Caulfield, John C. Powers, Stephen J. Pickett, John A. de León, Adalberto A. Pérez Birkett, Michael A. Borges, Lígia Miranda Ferreira |
spellingShingle |
de Oliveira Filho, Jaires Gomes Sarria, André Lucio Franceschini Ferreira, Lorena Lopes Caulfield, John C. Powers, Stephen J. Pickett, John A. de León, Adalberto A. Pérez Birkett, Michael A. Borges, Lígia Miranda Ferreira Quantification of brown dog tick repellents, 2-hexanone and benzaldehyde, and release from tick-resistant beagles, Canis lupus familiaris |
author_facet |
de Oliveira Filho, Jaires Gomes Sarria, André Lucio Franceschini Ferreira, Lorena Lopes Caulfield, John C. Powers, Stephen J. Pickett, John A. de León, Adalberto A. Pérez Birkett, Michael A. Borges, Lígia Miranda Ferreira |
author_sort |
de Oliveira Filho, Jaires Gomes |
title |
Quantification of brown dog tick repellents, 2-hexanone and benzaldehyde, and release from tick-resistant beagles, Canis lupus familiaris |
title_short |
Quantification of brown dog tick repellents, 2-hexanone and benzaldehyde, and release from tick-resistant beagles, Canis lupus familiaris |
title_full |
Quantification of brown dog tick repellents, 2-hexanone and benzaldehyde, and release from tick-resistant beagles, Canis lupus familiaris |
title_fullStr |
Quantification of brown dog tick repellents, 2-hexanone and benzaldehyde, and release from tick-resistant beagles, Canis lupus familiaris |
title_full_unstemmed |
Quantification of brown dog tick repellents, 2-hexanone and benzaldehyde, and release from tick-resistant beagles, Canis lupus familiaris |
title_sort |
quantification of brown dog tick repellents, 2-hexanone and benzaldehyde, and release from tick-resistant beagles, canis lupus familiaris |
publisher |
Elsevier |
publishDate |
2016 |
url |
https://orca.cardiff.ac.uk/id/eprint/106848/ https://doi.org/10.1016/j.jchromb.2016.03.014 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
de Oliveira Filho, Jaires Gomes, Sarria, André Lucio Franceschini, Ferreira, Lorena Lopes, Caulfield, John C., Powers, Stephen J., Pickett, John A. https://orca.cardiff.ac.uk/view/cardiffauthors/A23568735.html orcid:0000-0002-1008-6595 orcid:0000-0002-1008-6595, de León, Adalberto A. Pérez, Birkett, Michael A. and Borges, Lígia Miranda Ferreira 2016. Quantification of brown dog tick repellents, 2-hexanone and benzaldehyde, and release from tick-resistant beagles, Canis lupus familiaris. Journal of Chromatography B 1022 , pp. 64-69. 10.1016/j.jchromb.2016.03.014 https://doi.org/10.1016/j.jchromb.2016.03.014 doi:10.1016/j.jchromb.2016.03.014 |
op_doi |
https://doi.org/10.1016/j.jchromb.2016.03.014 |
container_title |
Journal of Chromatography B |
container_volume |
1022 |
container_start_page |
64 |
op_container_end_page |
69 |
_version_ |
1766385797962924032 |