Constraints in the colonization of natural and engineered subterranean igneous rock aquifers by aerobic methane-oxidizing bacteria inferred by culture analysis
The aerobic methane-oxidizing bacteria (MOB) are suggested to be important for the removal of oxygen from subterranean aquifers that become oxygenated by natural and engineering processes. This is primarily because MOB are ubiquitous in the environment and in addition reduce oxygen efficiently. The...
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ftunivcardiff:oai:https://orca.cardiff.ac.uk:105877 2023-05-15T16:13:04+02:00 Constraints in the colonization of natural and engineered subterranean igneous rock aquifers by aerobic methane-oxidizing bacteria inferred by culture analysis Chi Fru, Ernest 2008-09-01 https://orca.cardiff.ac.uk/id/eprint/105877/ https://doi.org/10.1111/j.1472-4669.2008.00164.x unknown Wiley Chi Fru, Ernest https://orca.cardiff.ac.uk/view/cardiffauthors/A2271334S.html orcid:0000-0003-2673-0565 orcid:0000-0003-2673-0565 2008. Constraints in the colonization of natural and engineered subterranean igneous rock aquifers by aerobic methane-oxidizing bacteria inferred by culture analysis. Geobiology 6 (4) , pp. 365-375. 10.1111/j.1472-4669.2008.00164.x https://doi.org/10.1111/j.1472-4669.2008.00164.x doi:10.1111/j.1472-4669.2008.00164.x Article PeerReviewed 2008 ftunivcardiff https://doi.org/10.1111/j.1472-4669.2008.00164.x 2022-11-03T23:43:39Z The aerobic methane-oxidizing bacteria (MOB) are suggested to be important for the removal of oxygen from subterranean aquifers that become oxygenated by natural and engineering processes. This is primarily because MOB are ubiquitous in the environment and in addition reduce oxygen efficiently. The biogeochemical factors that will control the success of the aerobic MOB in these kinds of underground aquifers remain unknown. In this study, viable and cultivable MOB occurring at natural and engineered deep granitic aquifers targeted for the disposal of spent nuclear fuel (SNF) in the Fennoscandian Shield (~3–1000 m) were enumerated. The numbers were correlated with in situ salinity, methane concentrations, conductivity, pH, and depth. A mixed population habiting freshwater aquifers (~3–20 m), a potential source for the inoculation of MOB into the deeper aquifers was tested for tolerance to NaCl, temperature, pH, and an ability to produce cysts and exospores. Extrapolations show that due to changing in situ parameters (salinity, conductivity, and pH), the numbers of MOB in the aquifers dropped quickly with depth. A positive correlation between the most probable numbers of MOB and methane concentrations was observed. Furthermore, the tolerance-based tests of cultured strains indicated that the MOB in the shallow aquifers thrived best in mesophilic and neutrophilic conditions as opposed to the hyperthermophilic and alkaliphilic conditions expected to develop in an engineered subterranean SNF repository. Overall, the survival of the MOB both quantitatively and physiologically in the granitic aquifers was under the strong influence of biogeochemical factors that are strongly depth-dependent. Article in Journal/Newspaper Fennoscandian Cardiff University: ORCA (Online Research @ Cardiff) Geobiology 6 4 365 375 |
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Cardiff University: ORCA (Online Research @ Cardiff) |
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description |
The aerobic methane-oxidizing bacteria (MOB) are suggested to be important for the removal of oxygen from subterranean aquifers that become oxygenated by natural and engineering processes. This is primarily because MOB are ubiquitous in the environment and in addition reduce oxygen efficiently. The biogeochemical factors that will control the success of the aerobic MOB in these kinds of underground aquifers remain unknown. In this study, viable and cultivable MOB occurring at natural and engineered deep granitic aquifers targeted for the disposal of spent nuclear fuel (SNF) in the Fennoscandian Shield (~3–1000 m) were enumerated. The numbers were correlated with in situ salinity, methane concentrations, conductivity, pH, and depth. A mixed population habiting freshwater aquifers (~3–20 m), a potential source for the inoculation of MOB into the deeper aquifers was tested for tolerance to NaCl, temperature, pH, and an ability to produce cysts and exospores. Extrapolations show that due to changing in situ parameters (salinity, conductivity, and pH), the numbers of MOB in the aquifers dropped quickly with depth. A positive correlation between the most probable numbers of MOB and methane concentrations was observed. Furthermore, the tolerance-based tests of cultured strains indicated that the MOB in the shallow aquifers thrived best in mesophilic and neutrophilic conditions as opposed to the hyperthermophilic and alkaliphilic conditions expected to develop in an engineered subterranean SNF repository. Overall, the survival of the MOB both quantitatively and physiologically in the granitic aquifers was under the strong influence of biogeochemical factors that are strongly depth-dependent. |
format |
Article in Journal/Newspaper |
author |
Chi Fru, Ernest |
spellingShingle |
Chi Fru, Ernest Constraints in the colonization of natural and engineered subterranean igneous rock aquifers by aerobic methane-oxidizing bacteria inferred by culture analysis |
author_facet |
Chi Fru, Ernest |
author_sort |
Chi Fru, Ernest |
title |
Constraints in the colonization of natural and engineered subterranean igneous rock aquifers by aerobic methane-oxidizing bacteria inferred by culture analysis |
title_short |
Constraints in the colonization of natural and engineered subterranean igneous rock aquifers by aerobic methane-oxidizing bacteria inferred by culture analysis |
title_full |
Constraints in the colonization of natural and engineered subterranean igneous rock aquifers by aerobic methane-oxidizing bacteria inferred by culture analysis |
title_fullStr |
Constraints in the colonization of natural and engineered subterranean igneous rock aquifers by aerobic methane-oxidizing bacteria inferred by culture analysis |
title_full_unstemmed |
Constraints in the colonization of natural and engineered subterranean igneous rock aquifers by aerobic methane-oxidizing bacteria inferred by culture analysis |
title_sort |
constraints in the colonization of natural and engineered subterranean igneous rock aquifers by aerobic methane-oxidizing bacteria inferred by culture analysis |
publisher |
Wiley |
publishDate |
2008 |
url |
https://orca.cardiff.ac.uk/id/eprint/105877/ https://doi.org/10.1111/j.1472-4669.2008.00164.x |
genre |
Fennoscandian |
genre_facet |
Fennoscandian |
op_relation |
Chi Fru, Ernest https://orca.cardiff.ac.uk/view/cardiffauthors/A2271334S.html orcid:0000-0003-2673-0565 orcid:0000-0003-2673-0565 2008. Constraints in the colonization of natural and engineered subterranean igneous rock aquifers by aerobic methane-oxidizing bacteria inferred by culture analysis. Geobiology 6 (4) , pp. 365-375. 10.1111/j.1472-4669.2008.00164.x https://doi.org/10.1111/j.1472-4669.2008.00164.x doi:10.1111/j.1472-4669.2008.00164.x |
op_doi |
https://doi.org/10.1111/j.1472-4669.2008.00164.x |
container_title |
Geobiology |
container_volume |
6 |
container_issue |
4 |
container_start_page |
365 |
op_container_end_page |
375 |
_version_ |
1765998681872400384 |