Adaptation of Proteins to the Cold in Antarctic Fish: A Role for Methionine?
The evolution of antifreeze glycoproteins has enabled notothenioid fish to flourish in the freezing waters of the Southern Ocean. Whereas successful at the biodiversity level to life in the cold, paradoxically at the cellular level these stenothermal animals have problems producing, folding, and deg...
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ftunivcam:oai:www.repository.cam.ac.uk:1810/289437 2024-02-04T09:54:03+01:00 Adaptation of Proteins to the Cold in Antarctic Fish: A Role for Methionine? Berthelot, Camille Clarke, Jane Desvignes, Thomas William Detrich, H Flicek, Paul Peck, Lloyd S Peters, Michael Postlethwait, John H Clark, Melody S 2019-01-01 application/pdf https://www.repository.cam.ac.uk/handle/1810/289437 https://doi.org/10.17863/CAM.36686 eng eng Oxford University Press (OUP) https://academic.oup.com/gbe/article/11/1/220/5215157 Genome Biol Evol https://www.repository.cam.ac.uk/handle/1810/289437 doi:10.17863/CAM.36686 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ Acclimatization Animals Antarctic Regions Evolution Molecular Fish Proteins Freezing Methionine Perciformes Protein Folding Transcriptome Article 2019 ftunivcam https://doi.org/10.17863/CAM.36686 2024-01-11T23:20:30Z The evolution of antifreeze glycoproteins has enabled notothenioid fish to flourish in the freezing waters of the Southern Ocean. Whereas successful at the biodiversity level to life in the cold, paradoxically at the cellular level these stenothermal animals have problems producing, folding, and degrading proteins at their ambient temperatures of -1.86 °C. In this first multi-species transcriptome comparison of the amino acid composition of notothenioid proteins with temperate teleost proteins, we show that, unlike psychrophilic bacteria, Antarctic fish provide little evidence for the mass alteration of protein amino acid composition to enhance protein folding and reduce protein denaturation in the cold. The exception was the significant overrepresentation of positions where leucine in temperate fish proteins was replaced by methionine in the notothenioid orthologues. We hypothesize that these extra methionines have been preferentially assimilated into the genome to act as redox sensors in the highly oxygenated waters of the Southern Ocean. This redox hypothesis is supported by analyses of notothenioids showing enrichment of genes associated with responses to environmental stress, particularly reactive oxygen species. So overall, although notothenioid fish show cold-associated problems with protein homeostasis, they may have modified only a selected number of biochemical pathways to work efficiently below 0 °C. Even a slight warming of the Southern Ocean might disrupt the critical functions of this handful of key pathways with considerable impacts for the functioning of this ecosystem in the future. Article in Journal/Newspaper Antarc* Antarctic Southern Ocean Apollo - University of Cambridge Repository Antarctic Southern Ocean |
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Open Polar |
collection |
Apollo - University of Cambridge Repository |
op_collection_id |
ftunivcam |
language |
English |
topic |
Acclimatization Animals Antarctic Regions Evolution Molecular Fish Proteins Freezing Methionine Perciformes Protein Folding Transcriptome |
spellingShingle |
Acclimatization Animals Antarctic Regions Evolution Molecular Fish Proteins Freezing Methionine Perciformes Protein Folding Transcriptome Berthelot, Camille Clarke, Jane Desvignes, Thomas William Detrich, H Flicek, Paul Peck, Lloyd S Peters, Michael Postlethwait, John H Clark, Melody S Adaptation of Proteins to the Cold in Antarctic Fish: A Role for Methionine? |
topic_facet |
Acclimatization Animals Antarctic Regions Evolution Molecular Fish Proteins Freezing Methionine Perciformes Protein Folding Transcriptome |
description |
The evolution of antifreeze glycoproteins has enabled notothenioid fish to flourish in the freezing waters of the Southern Ocean. Whereas successful at the biodiversity level to life in the cold, paradoxically at the cellular level these stenothermal animals have problems producing, folding, and degrading proteins at their ambient temperatures of -1.86 °C. In this first multi-species transcriptome comparison of the amino acid composition of notothenioid proteins with temperate teleost proteins, we show that, unlike psychrophilic bacteria, Antarctic fish provide little evidence for the mass alteration of protein amino acid composition to enhance protein folding and reduce protein denaturation in the cold. The exception was the significant overrepresentation of positions where leucine in temperate fish proteins was replaced by methionine in the notothenioid orthologues. We hypothesize that these extra methionines have been preferentially assimilated into the genome to act as redox sensors in the highly oxygenated waters of the Southern Ocean. This redox hypothesis is supported by analyses of notothenioids showing enrichment of genes associated with responses to environmental stress, particularly reactive oxygen species. So overall, although notothenioid fish show cold-associated problems with protein homeostasis, they may have modified only a selected number of biochemical pathways to work efficiently below 0 °C. Even a slight warming of the Southern Ocean might disrupt the critical functions of this handful of key pathways with considerable impacts for the functioning of this ecosystem in the future. |
format |
Article in Journal/Newspaper |
author |
Berthelot, Camille Clarke, Jane Desvignes, Thomas William Detrich, H Flicek, Paul Peck, Lloyd S Peters, Michael Postlethwait, John H Clark, Melody S |
author_facet |
Berthelot, Camille Clarke, Jane Desvignes, Thomas William Detrich, H Flicek, Paul Peck, Lloyd S Peters, Michael Postlethwait, John H Clark, Melody S |
author_sort |
Berthelot, Camille |
title |
Adaptation of Proteins to the Cold in Antarctic Fish: A Role for Methionine? |
title_short |
Adaptation of Proteins to the Cold in Antarctic Fish: A Role for Methionine? |
title_full |
Adaptation of Proteins to the Cold in Antarctic Fish: A Role for Methionine? |
title_fullStr |
Adaptation of Proteins to the Cold in Antarctic Fish: A Role for Methionine? |
title_full_unstemmed |
Adaptation of Proteins to the Cold in Antarctic Fish: A Role for Methionine? |
title_sort |
adaptation of proteins to the cold in antarctic fish: a role for methionine? |
publisher |
Oxford University Press (OUP) |
publishDate |
2019 |
url |
https://www.repository.cam.ac.uk/handle/1810/289437 https://doi.org/10.17863/CAM.36686 |
geographic |
Antarctic Southern Ocean |
geographic_facet |
Antarctic Southern Ocean |
genre |
Antarc* Antarctic Southern Ocean |
genre_facet |
Antarc* Antarctic Southern Ocean |
op_relation |
https://www.repository.cam.ac.uk/handle/1810/289437 doi:10.17863/CAM.36686 |
op_rights |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.17863/CAM.36686 |
_version_ |
1789970280371716096 |