Comprehensive annotation and evolutionary insights into the canine (Canis lupus familiaris) antigen receptor loci
Dogs are an excellent model for human disease. For example, the treatment of canine lymphoma has been predictive of the human response to that treatment. However, an incomplete picture of canine (Canis lupus familiaris) immunoglobulin (IG) and T cell receptor (TR) - or antigen receptor (AR) - gene l...
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ftunivcam:oai:www.repository.cam.ac.uk:1810/267510 2024-01-14T10:06:02+01:00 Comprehensive annotation and evolutionary insights into the canine (Canis lupus familiaris) antigen receptor loci Martin, JNE Ponstingl, H Lefranc, M Archer, J Sargan, D Bradley, A 2018-04 application/pdf https://www.repository.cam.ac.uk/handle/1810/267510 https://doi.org/10.17863/CAM.13449 eng eng Springer Nature http://dx.doi.org/10.1007/s00251-017-1028-0 Immunogenetics https://www.repository.cam.ac.uk/handle/1810/267510 doi:10.17863/CAM.13449 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ Canine Antigen receptor Immunoglobulin T cell receptor Article 2018 ftunivcam https://doi.org/10.17863/CAM.13449 2023-12-21T23:21:13Z Dogs are an excellent model for human disease. For example, the treatment of canine lymphoma has been predictive of the human response to that treatment. However, an incomplete picture of canine (Canis lupus familiaris) immunoglobulin (IG) and T cell receptor (TR) - or antigen receptor (AR) - gene loci has restricted their utility. This work advances the annotation of the canine AR loci and looks into breed-specific features of the loci. Bioinformatic analysis of unbiased RNA sequence data was used to complete the annotation of the canine AR genes. This annotation was used to query 107 whole genome sequences from 19 breeds and identified over 5,500 alleles across the 550 genes of the seven AR loci: the IG heavy, kappa, and lambda loci; and the TR alpha, beta, gamma, and delta loci. Of note was the discovery that half of the IGK variable (V) genes were located downstream of, and inverted with respect to, the rest of the locus. Analysis of the germline sequences of all the AR V genes identified greater conservation between dog and human than mouse with either. This work brings our understanding of the genetic diversity and expression of AR in dogs to the same completeness as that of mice and men, making it the third species to have all AR loci comprehensively and accurately annotated. The large number of germline sequences serves as a reference for future studies, and has allowed statistically powerful conclusions to be drawn on the pressures that have shaped these loci. This work was supported by funding from the BBSRC and the Wellcome Trust. Article in Journal/Newspaper Canis lupus Apollo - University of Cambridge Repository Lambda ENVELOPE(-62.983,-62.983,-64.300,-64.300) |
institution |
Open Polar |
collection |
Apollo - University of Cambridge Repository |
op_collection_id |
ftunivcam |
language |
English |
topic |
Canine Antigen receptor Immunoglobulin T cell receptor |
spellingShingle |
Canine Antigen receptor Immunoglobulin T cell receptor Martin, JNE Ponstingl, H Lefranc, M Archer, J Sargan, D Bradley, A Comprehensive annotation and evolutionary insights into the canine (Canis lupus familiaris) antigen receptor loci |
topic_facet |
Canine Antigen receptor Immunoglobulin T cell receptor |
description |
Dogs are an excellent model for human disease. For example, the treatment of canine lymphoma has been predictive of the human response to that treatment. However, an incomplete picture of canine (Canis lupus familiaris) immunoglobulin (IG) and T cell receptor (TR) - or antigen receptor (AR) - gene loci has restricted their utility. This work advances the annotation of the canine AR loci and looks into breed-specific features of the loci. Bioinformatic analysis of unbiased RNA sequence data was used to complete the annotation of the canine AR genes. This annotation was used to query 107 whole genome sequences from 19 breeds and identified over 5,500 alleles across the 550 genes of the seven AR loci: the IG heavy, kappa, and lambda loci; and the TR alpha, beta, gamma, and delta loci. Of note was the discovery that half of the IGK variable (V) genes were located downstream of, and inverted with respect to, the rest of the locus. Analysis of the germline sequences of all the AR V genes identified greater conservation between dog and human than mouse with either. This work brings our understanding of the genetic diversity and expression of AR in dogs to the same completeness as that of mice and men, making it the third species to have all AR loci comprehensively and accurately annotated. The large number of germline sequences serves as a reference for future studies, and has allowed statistically powerful conclusions to be drawn on the pressures that have shaped these loci. This work was supported by funding from the BBSRC and the Wellcome Trust. |
format |
Article in Journal/Newspaper |
author |
Martin, JNE Ponstingl, H Lefranc, M Archer, J Sargan, D Bradley, A |
author_facet |
Martin, JNE Ponstingl, H Lefranc, M Archer, J Sargan, D Bradley, A |
author_sort |
Martin, JNE |
title |
Comprehensive annotation and evolutionary insights into the canine (Canis lupus familiaris) antigen receptor loci |
title_short |
Comprehensive annotation and evolutionary insights into the canine (Canis lupus familiaris) antigen receptor loci |
title_full |
Comprehensive annotation and evolutionary insights into the canine (Canis lupus familiaris) antigen receptor loci |
title_fullStr |
Comprehensive annotation and evolutionary insights into the canine (Canis lupus familiaris) antigen receptor loci |
title_full_unstemmed |
Comprehensive annotation and evolutionary insights into the canine (Canis lupus familiaris) antigen receptor loci |
title_sort |
comprehensive annotation and evolutionary insights into the canine (canis lupus familiaris) antigen receptor loci |
publisher |
Springer Nature |
publishDate |
2018 |
url |
https://www.repository.cam.ac.uk/handle/1810/267510 https://doi.org/10.17863/CAM.13449 |
long_lat |
ENVELOPE(-62.983,-62.983,-64.300,-64.300) |
geographic |
Lambda |
geographic_facet |
Lambda |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
https://www.repository.cam.ac.uk/handle/1810/267510 doi:10.17863/CAM.13449 |
op_rights |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.17863/CAM.13449 |
_version_ |
1788060338476810240 |