Genomic characterization and virulence gene profiling of Erysipelothrix rhusiopathiae isolated from widespread muskox mortalities in the Canadian Arctic Archipelago
Abstract Background Muskoxen are important ecosystem components and provide food, economic opportunities, and cultural well-being for Indigenous communities in the Canadian Arctic. Between 2010 and 2021, Erysipelothrix rhusiopathiae was isolated from carcasses of muskoxen, caribou, a seal, and an Ar...
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ftunivcalgary:oai:prism.ucalgary.ca:1880/119218 2024-09-15T17:52:10+00:00 Genomic characterization and virulence gene profiling of Erysipelothrix rhusiopathiae isolated from widespread muskox mortalities in the Canadian Arctic Archipelago Seru, Lakshmi V. Forde, Taya L. Roberto-Charron, Amélie Mavrot, Fabien Niu, Yan D. Kutz, Susan J. 2024-07-21T00:05:33Z application/pdf https://hdl.handle.net/1880/119218 https://doi.org/10.1186/s12864-024-10592-9 https://doi.org/10.11575/PRISM/46814 en eng BMC Genomics. 2024 Jul 14;25(1):691 https://doi.org/10.1186/s12864-024-10592-9 https://hdl.handle.net/1880/119218 https://doi.org/10.11575/PRISM/46814 The Author(s) Journal Article 2024 ftunivcalgary https://doi.org/10.1186/s12864-024-10592-910.11575/PRISM/46814 2024-07-30T23:46:17Z Abstract Background Muskoxen are important ecosystem components and provide food, economic opportunities, and cultural well-being for Indigenous communities in the Canadian Arctic. Between 2010 and 2021, Erysipelothrix rhusiopathiae was isolated from carcasses of muskoxen, caribou, a seal, and an Arctic fox during multiple large scale mortality events in the Canadian Arctic Archipelago. A single strain (‘Arctic clone’) of E. rhusiopathiae was associated with the mortalities on Banks, Victoria and Prince Patrick Islands, Northwest Territories and Nunavut, Canada (2010–2017). The objectives of this study were to (i) characterize the genomes of E. rhusiopathiae isolates obtained from more recent muskox mortalities in the Canadian Arctic in 2019 and 2021; (ii) identify and compare common virulence traits associated with the core genome and mobile genetic elements (i.e. pathogenicity islands and prophages) among Arctic clone versus other E. rhusiopathiae genomes; and iii) use pan-genome wide association studies (GWAS) to determine unique genetic contents of the Arctic clone that may encode virulence traits and that could be used for diagnostic purposes. Results Phylogenetic analyses revealed that the newly sequenced E. rhusiopathiae isolates from Ellesmere Island, Nunavut (2021) also belong to the Arctic clone. Of 17 virulence genes analysed among 28 Arctic clone isolates, four genes – adhesin, rhusiopathiae surface protein-A (rspA), choline binding protein-B (cbpB) and CDP-glycerol glycerophosphotransferase (tagF) – had amino acid sequence variants unique to this clone when compared to 31 other E. rhusiopathiae genomes. These genes encode proteins that facilitate E. rhusiopathiae to attach to the host endothelial cells and form biofilms. GWAS analyses using Scoary found several unique genes to be overrepresented in the Arctic clone. Conclusions The Arctic clone of E. rhusiopathiae was associated with multiple muskox mortalities spanning over a decade and multiple Arctic islands with distances over 1000 km, ... Article in Journal/Newspaper Arctic Archipelago Arctic Fox Arctic Canadian Arctic Archipelago Ellesmere Island muskox Northwest Territories Nunavut PRISM - University of Calgary Digital Repository |
institution |
Open Polar |
collection |
PRISM - University of Calgary Digital Repository |
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ftunivcalgary |
language |
English |
description |
Abstract Background Muskoxen are important ecosystem components and provide food, economic opportunities, and cultural well-being for Indigenous communities in the Canadian Arctic. Between 2010 and 2021, Erysipelothrix rhusiopathiae was isolated from carcasses of muskoxen, caribou, a seal, and an Arctic fox during multiple large scale mortality events in the Canadian Arctic Archipelago. A single strain (‘Arctic clone’) of E. rhusiopathiae was associated with the mortalities on Banks, Victoria and Prince Patrick Islands, Northwest Territories and Nunavut, Canada (2010–2017). The objectives of this study were to (i) characterize the genomes of E. rhusiopathiae isolates obtained from more recent muskox mortalities in the Canadian Arctic in 2019 and 2021; (ii) identify and compare common virulence traits associated with the core genome and mobile genetic elements (i.e. pathogenicity islands and prophages) among Arctic clone versus other E. rhusiopathiae genomes; and iii) use pan-genome wide association studies (GWAS) to determine unique genetic contents of the Arctic clone that may encode virulence traits and that could be used for diagnostic purposes. Results Phylogenetic analyses revealed that the newly sequenced E. rhusiopathiae isolates from Ellesmere Island, Nunavut (2021) also belong to the Arctic clone. Of 17 virulence genes analysed among 28 Arctic clone isolates, four genes – adhesin, rhusiopathiae surface protein-A (rspA), choline binding protein-B (cbpB) and CDP-glycerol glycerophosphotransferase (tagF) – had amino acid sequence variants unique to this clone when compared to 31 other E. rhusiopathiae genomes. These genes encode proteins that facilitate E. rhusiopathiae to attach to the host endothelial cells and form biofilms. GWAS analyses using Scoary found several unique genes to be overrepresented in the Arctic clone. Conclusions The Arctic clone of E. rhusiopathiae was associated with multiple muskox mortalities spanning over a decade and multiple Arctic islands with distances over 1000 km, ... |
format |
Article in Journal/Newspaper |
author |
Seru, Lakshmi V. Forde, Taya L. Roberto-Charron, Amélie Mavrot, Fabien Niu, Yan D. Kutz, Susan J. |
spellingShingle |
Seru, Lakshmi V. Forde, Taya L. Roberto-Charron, Amélie Mavrot, Fabien Niu, Yan D. Kutz, Susan J. Genomic characterization and virulence gene profiling of Erysipelothrix rhusiopathiae isolated from widespread muskox mortalities in the Canadian Arctic Archipelago |
author_facet |
Seru, Lakshmi V. Forde, Taya L. Roberto-Charron, Amélie Mavrot, Fabien Niu, Yan D. Kutz, Susan J. |
author_sort |
Seru, Lakshmi V. |
title |
Genomic characterization and virulence gene profiling of Erysipelothrix rhusiopathiae isolated from widespread muskox mortalities in the Canadian Arctic Archipelago |
title_short |
Genomic characterization and virulence gene profiling of Erysipelothrix rhusiopathiae isolated from widespread muskox mortalities in the Canadian Arctic Archipelago |
title_full |
Genomic characterization and virulence gene profiling of Erysipelothrix rhusiopathiae isolated from widespread muskox mortalities in the Canadian Arctic Archipelago |
title_fullStr |
Genomic characterization and virulence gene profiling of Erysipelothrix rhusiopathiae isolated from widespread muskox mortalities in the Canadian Arctic Archipelago |
title_full_unstemmed |
Genomic characterization and virulence gene profiling of Erysipelothrix rhusiopathiae isolated from widespread muskox mortalities in the Canadian Arctic Archipelago |
title_sort |
genomic characterization and virulence gene profiling of erysipelothrix rhusiopathiae isolated from widespread muskox mortalities in the canadian arctic archipelago |
publishDate |
2024 |
url |
https://hdl.handle.net/1880/119218 https://doi.org/10.1186/s12864-024-10592-9 https://doi.org/10.11575/PRISM/46814 |
genre |
Arctic Archipelago Arctic Fox Arctic Canadian Arctic Archipelago Ellesmere Island muskox Northwest Territories Nunavut |
genre_facet |
Arctic Archipelago Arctic Fox Arctic Canadian Arctic Archipelago Ellesmere Island muskox Northwest Territories Nunavut |
op_relation |
BMC Genomics. 2024 Jul 14;25(1):691 https://doi.org/10.1186/s12864-024-10592-9 https://hdl.handle.net/1880/119218 https://doi.org/10.11575/PRISM/46814 |
op_rights |
The Author(s) |
op_doi |
https://doi.org/10.1186/s12864-024-10592-910.11575/PRISM/46814 |
_version_ |
1810294251960926208 |