Thermophilic Endospore-Forming Bacteria as Models for Exploring Microbial Dispersal in Time and Space
Thermophilic endospore-forming bacteria – “ thermospores ” – are particularly useful model organisms for exploring microbial biogeography because they remain viable for long geologic time periods owing to a dormant state that confers resistant to extreme conditions. Using high temperature incubation...
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ftunivcalgary:oai:prism.ucalgary.ca:11023/4275 2023-08-27T04:10:52+02:00 Thermophilic Endospore-Forming Bacteria as Models for Exploring Microbial Dispersal in Time and Space Cramm, Margaret Hubert, Casey Dunfield, Peter Fox, Jeremy 2017 application/pdf http://hdl.handle.net/11023/4275 https://doi.org/10.11575/PRISM/28742 eng eng Graduate Studies University of Calgary Calgary Cramm, M. (2017). Thermophilic Endospore-Forming Bacteria as Models for Exploring Microbial Dispersal in Time and Space (Unpublished master's thesis). University of Calgary, Calgary, AB. doi:10.11575/PRISM/28742 http://dx.doi.org/10.11575/PRISM/28742 http://hdl.handle.net/11023/4275 University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission. Microbiology environmental microbiology microbial biogeography master thesis 2017 ftunivcalgary https://doi.org/10.11575/PRISM/28742 2023-08-06T06:31:23Z Thermophilic endospore-forming bacteria – “ thermospores ” – are particularly useful model organisms for exploring microbial biogeography because they remain viable for long geologic time periods owing to a dormant state that confers resistant to extreme conditions. Using high temperature incubation experiments and 16S rRNA gene amplicon sequencing, geographic and temporal thermospore dispersal in marine sediments was explored. Thermospores detected in surface sediments across the North Atlantic are likely to originate from multiple warm temperature habitats, and are viable in sediments buried ~15 000 years ago. These approaches also revealed thermospore viability following the extreme stress of prolonged -80°C exposure. Thermospore viability and dispersal on a scale of millions of years was explored in a 1.2 km long sediment core. Uneven thermospore germination posed a challenge for thermospore detection but their capacity for use as models of biological dispersal remains valid. Master Thesis North Atlantic PRISM - University of Calgary Digital Repository |
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Open Polar |
collection |
PRISM - University of Calgary Digital Repository |
op_collection_id |
ftunivcalgary |
language |
English |
topic |
Microbiology environmental microbiology microbial biogeography |
spellingShingle |
Microbiology environmental microbiology microbial biogeography Cramm, Margaret Thermophilic Endospore-Forming Bacteria as Models for Exploring Microbial Dispersal in Time and Space |
topic_facet |
Microbiology environmental microbiology microbial biogeography |
description |
Thermophilic endospore-forming bacteria – “ thermospores ” – are particularly useful model organisms for exploring microbial biogeography because they remain viable for long geologic time periods owing to a dormant state that confers resistant to extreme conditions. Using high temperature incubation experiments and 16S rRNA gene amplicon sequencing, geographic and temporal thermospore dispersal in marine sediments was explored. Thermospores detected in surface sediments across the North Atlantic are likely to originate from multiple warm temperature habitats, and are viable in sediments buried ~15 000 years ago. These approaches also revealed thermospore viability following the extreme stress of prolonged -80°C exposure. Thermospore viability and dispersal on a scale of millions of years was explored in a 1.2 km long sediment core. Uneven thermospore germination posed a challenge for thermospore detection but their capacity for use as models of biological dispersal remains valid. |
author2 |
Hubert, Casey Dunfield, Peter Fox, Jeremy |
format |
Master Thesis |
author |
Cramm, Margaret |
author_facet |
Cramm, Margaret |
author_sort |
Cramm, Margaret |
title |
Thermophilic Endospore-Forming Bacteria as Models for Exploring Microbial Dispersal in Time and Space |
title_short |
Thermophilic Endospore-Forming Bacteria as Models for Exploring Microbial Dispersal in Time and Space |
title_full |
Thermophilic Endospore-Forming Bacteria as Models for Exploring Microbial Dispersal in Time and Space |
title_fullStr |
Thermophilic Endospore-Forming Bacteria as Models for Exploring Microbial Dispersal in Time and Space |
title_full_unstemmed |
Thermophilic Endospore-Forming Bacteria as Models for Exploring Microbial Dispersal in Time and Space |
title_sort |
thermophilic endospore-forming bacteria as models for exploring microbial dispersal in time and space |
publisher |
Graduate Studies |
publishDate |
2017 |
url |
http://hdl.handle.net/11023/4275 https://doi.org/10.11575/PRISM/28742 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
Cramm, M. (2017). Thermophilic Endospore-Forming Bacteria as Models for Exploring Microbial Dispersal in Time and Space (Unpublished master's thesis). University of Calgary, Calgary, AB. doi:10.11575/PRISM/28742 http://dx.doi.org/10.11575/PRISM/28742 http://hdl.handle.net/11023/4275 |
op_rights |
University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission. |
op_doi |
https://doi.org/10.11575/PRISM/28742 |
_version_ |
1775353222540558336 |