Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis
The Pleuronectiformes order, which includes several commercially-important species, has undergone extensive chromosome evolution. One of these species is Solea senegalensis, a flatfish with 2n = 42 chromosomes. In this study, a cytogenomics approach and integration with previous maps was applied to...
Published in: | International Journal of Molecular Sciences |
---|---|
Main Authors: | , , , , , , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI
2022
|
Subjects: | |
Online Access: | http://hdl.handle.net/10498/27227 https://doi.org/10.3390/ijms23105353 |
id |
ftunivcadiz:oai:rodin.uca.es:10498/27227 |
---|---|
record_format |
openpolar |
spelling |
ftunivcadiz:oai:rodin.uca.es:10498/27227 2023-05-15T18:15:51+02:00 Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis Ramírez Torres, Daniel Rodríguez Jiménez, María Esther Cross Pacheco, Ismael Arias Pérez, Alberto Merlo Torres, Manuel Alejandro Anaya, Marco Martínez, Paulino Robles, Francisca Ruiz Rejón, C. Rebordinos González, Laureana Biomedicina, Biotecnología y Salud Pública 2022-07-22T11:40:18Z application/pdf http://hdl.handle.net/10498/27227 https://doi.org/10.3390/ijms23105353 eng eng MDPI info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096847-B-C21/ES/ESTUDIOS GENOMICOS Y DE DETERMINACION SEXUAL EN EL LENGUADO (SOLEA SENEGALENSIS) MEDIANTE INTEGRACION DE MAPAS/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096847-B-C22/ES/DESARROLLO DEL MAPA DE LIGAMIENTO DIPLOIDE DEL LENGUADO Y CARACTERIZACION DE SECUENCIAS REPETIDAS Y GENES CON EXPRESION DIFERENCIAL ENTRE SEXOS/ 1422-0067 http://hdl.handle.net/10498/27227 doi:10.3390/ijms23105353 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess CC-BY International Journal of Molecular Sciences, Vol. 23, Núm. 10 Solea senegalensis pleuronectiformes genetic maps cytogenomics chromosome evolution karyotype repetitive sequences comparative genomics info:eu-repo/semantics/article 2022 ftunivcadiz https://doi.org/10.3390/ijms23105353 2023-02-24T07:59:23Z The Pleuronectiformes order, which includes several commercially-important species, has undergone extensive chromosome evolution. One of these species is Solea senegalensis, a flatfish with 2n = 42 chromosomes. In this study, a cytogenomics approach and integration with previous maps was applied to characterize the karyotype of the species. Synteny analysis of S. senegalensis was carried out using two flatfish as a reference: Cynoglossus semilaevis and Scophthalmus maximus. Most S. senegalensis chromosomes (or chromosome arms for metacentrics and submetacentrics) showed a one-to-one macrosyntenic pattern with the other two species. In addition, we studied how repetitive sequences could have played a role in the evolution of S. senegalensis bi-armed (3, and 5-9) and acrocentric (11, 12 and 16) chromosomes, which showed the highest rearrangements compared with the reference species. A higher abundance of TEs (Transposable Elements) and other repeated elements was observed adjacent to telomeric regions on chromosomes 3, 7, 9 and 16. However, on chromosome 11, a greater abundance of DNA transposons was detected in interstitial BACs. This chromosome is syntenic with several chromosomes of the other two flatfish species, suggesting rearrangements during its evolution. A similar situation was also found on chromosome 16 (for microsatellites and low complexity sequences), but not for TEs (retroelements and DNA transposons). These differences in the distribution and abundance of repetitive elements in chromosomes that have undergone remodeling processes during the course of evolution also suggest a possible role for simple repeat sequences in rearranged regions. Article in Journal/Newspaper Scophthalmus maximus RODIN - Repositorio de Objetos de Docencia e Investigación de la Universidad de Cádiz International Journal of Molecular Sciences 23 10 5353 |
institution |
Open Polar |
collection |
RODIN - Repositorio de Objetos de Docencia e Investigación de la Universidad de Cádiz |
op_collection_id |
ftunivcadiz |
language |
English |
topic |
Solea senegalensis pleuronectiformes genetic maps cytogenomics chromosome evolution karyotype repetitive sequences comparative genomics |
spellingShingle |
Solea senegalensis pleuronectiformes genetic maps cytogenomics chromosome evolution karyotype repetitive sequences comparative genomics Ramírez Torres, Daniel Rodríguez Jiménez, María Esther Cross Pacheco, Ismael Arias Pérez, Alberto Merlo Torres, Manuel Alejandro Anaya, Marco Martínez, Paulino Robles, Francisca Ruiz Rejón, C. Rebordinos González, Laureana Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis |
topic_facet |
Solea senegalensis pleuronectiformes genetic maps cytogenomics chromosome evolution karyotype repetitive sequences comparative genomics |
description |
The Pleuronectiformes order, which includes several commercially-important species, has undergone extensive chromosome evolution. One of these species is Solea senegalensis, a flatfish with 2n = 42 chromosomes. In this study, a cytogenomics approach and integration with previous maps was applied to characterize the karyotype of the species. Synteny analysis of S. senegalensis was carried out using two flatfish as a reference: Cynoglossus semilaevis and Scophthalmus maximus. Most S. senegalensis chromosomes (or chromosome arms for metacentrics and submetacentrics) showed a one-to-one macrosyntenic pattern with the other two species. In addition, we studied how repetitive sequences could have played a role in the evolution of S. senegalensis bi-armed (3, and 5-9) and acrocentric (11, 12 and 16) chromosomes, which showed the highest rearrangements compared with the reference species. A higher abundance of TEs (Transposable Elements) and other repeated elements was observed adjacent to telomeric regions on chromosomes 3, 7, 9 and 16. However, on chromosome 11, a greater abundance of DNA transposons was detected in interstitial BACs. This chromosome is syntenic with several chromosomes of the other two flatfish species, suggesting rearrangements during its evolution. A similar situation was also found on chromosome 16 (for microsatellites and low complexity sequences), but not for TEs (retroelements and DNA transposons). These differences in the distribution and abundance of repetitive elements in chromosomes that have undergone remodeling processes during the course of evolution also suggest a possible role for simple repeat sequences in rearranged regions. |
author2 |
Biomedicina, Biotecnología y Salud Pública |
format |
Article in Journal/Newspaper |
author |
Ramírez Torres, Daniel Rodríguez Jiménez, María Esther Cross Pacheco, Ismael Arias Pérez, Alberto Merlo Torres, Manuel Alejandro Anaya, Marco Martínez, Paulino Robles, Francisca Ruiz Rejón, C. Rebordinos González, Laureana |
author_facet |
Ramírez Torres, Daniel Rodríguez Jiménez, María Esther Cross Pacheco, Ismael Arias Pérez, Alberto Merlo Torres, Manuel Alejandro Anaya, Marco Martínez, Paulino Robles, Francisca Ruiz Rejón, C. Rebordinos González, Laureana |
author_sort |
Ramírez Torres, Daniel |
title |
Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis |
title_short |
Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis |
title_full |
Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis |
title_fullStr |
Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis |
title_full_unstemmed |
Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis |
title_sort |
integration of maps enables a cytogenomics analysis of the complete karyotype in solea senegalensis |
publisher |
MDPI |
publishDate |
2022 |
url |
http://hdl.handle.net/10498/27227 https://doi.org/10.3390/ijms23105353 |
genre |
Scophthalmus maximus |
genre_facet |
Scophthalmus maximus |
op_source |
International Journal of Molecular Sciences, Vol. 23, Núm. 10 |
op_relation |
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096847-B-C21/ES/ESTUDIOS GENOMICOS Y DE DETERMINACION SEXUAL EN EL LENGUADO (SOLEA SENEGALENSIS) MEDIANTE INTEGRACION DE MAPAS/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096847-B-C22/ES/DESARROLLO DEL MAPA DE LIGAMIENTO DIPLOIDE DEL LENGUADO Y CARACTERIZACION DE SECUENCIAS REPETIDAS Y GENES CON EXPRESION DIFERENCIAL ENTRE SEXOS/ 1422-0067 http://hdl.handle.net/10498/27227 doi:10.3390/ijms23105353 |
op_rights |
Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/ijms23105353 |
container_title |
International Journal of Molecular Sciences |
container_volume |
23 |
container_issue |
10 |
container_start_page |
5353 |
_version_ |
1766189082649559040 |