Faecal microbiota and antibiotic resistance genes in migratory waterbirds with contrasting habitat use.
Migratory birds may have a vital role in the spread of antimicrobial resistance across habitats and regions, but empirical data remain scarce. We investigated differences in the gut microbiome composition and the abundance of antibiotic resistance genes (ARGs) in faeces from four migratory waterbird...
Published in: | Science of The Total Environment |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Online Access: | http://hdl.handle.net/10498/24955 https://doi.org/10.1016/j.scitotenv.2021.146872 |
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ftunivcadiz:oai:rodin.uca.es:10498/24955 2023-05-15T17:07:56+02:00 Faecal microbiota and antibiotic resistance genes in migratory waterbirds with contrasting habitat use. Jarma, Dayana Ayelen Sánchez, Marta I. Green, Andy J. Peralta-Sánchez, Juan Manuel Hortas Rodríguez-Pascual, Francisco Sánchez-Melsió, Alexandre Borrego, Carles M. Biología 2021-06-16T10:04:51Z application/pdf http://hdl.handle.net/10498/24955 https://doi.org/10.1016/j.scitotenv.2021.146872 eng eng ELSEVIER info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108962GB-C21/ES/AVES ACUATICAS COMO VECTORES DE DISPERSION DE RESISTENCIAS: PAPEL DE LA ECOLOGIA Y LA CONTAMINACION AMBIENTAL/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108962GB-C22/ES/AVES ACUATICAS COMO RESERVORIOS DE RESISTENCIAS A ANTIBIOTICOS/ 1879-1026 http://hdl.handle.net/10498/24955 doi:10.1016/j.scitotenv.2021.146872 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess CC-BY-NC-ND Science of the Total Environment 783 (2021) 146872 info:eu-repo/semantics/article 2021 ftunivcadiz https://doi.org/10.1016/j.scitotenv.2021.146872 2023-02-24T07:59:07Z Migratory birds may have a vital role in the spread of antimicrobial resistance across habitats and regions, but empirical data remain scarce. We investigated differences in the gut microbiome composition and the abundance of antibiotic resistance genes (ARGs) in faeces from four migratory waterbirds wintering in South-West Spain that differ in their habitat use. The white stork Ciconia ciconia and lesser black-backed gull Larus fuscus are omnivorous and opportunistic birds that use highly anthropogenic habitats such as landfills and urban areas. The greylag goose Anser anser and common crane Grus grus are herbivores and use more natural habitats. Fresh faeces from 15 individuals of each species were analysed to assess the composition of bacterial communities using 16S rRNA amplicon-targeted sequencing, and to quantify the abundance of the Class I integron integrase gene (intI1) as well as genes encoding resistance to sulfonamides (sul1), beta-lactams (blaTEM, blaKPC and blaNDM), tetracyclines (tetW), fluoroquinolones (qnrS), and colistin (mcr-1) using qPCR. Bacterial communities in gull faeces were the richest and most diverse. Beta diversity analysis showed segregation in faecal communities between bird species, but those from storks and gulls were the most similar, these being the species that regularly feed in landfills. Potential bacterial pathogens identified in faeces differed significantly between bird species, with higher relative abundance in gulls. Faeces from birds that feed in landfills (stork and gull) contained a significantly higher abundance of ARGs (sul1, blaTEM, and tetW). Genes conferring resistance to last resort antibiotics such as carbapenems (blaKPC) and colistin (mcr-1) were only observed in faeces from gulls. These results show that these bird species are reservoirs of antimicrobial resistant bacteria and suggest that waterbirds may disseminate antibiotic resistance across environments (e.g., from landfills to ricefields or water supplies), and thus constitute a risk for their further ... Article in Journal/Newspaper Lesser black-backed gull RODIN - Repositorio de Objetos de Docencia e Investigación de la Universidad de Cádiz Science of The Total Environment 783 146872 |
institution |
Open Polar |
collection |
RODIN - Repositorio de Objetos de Docencia e Investigación de la Universidad de Cádiz |
op_collection_id |
ftunivcadiz |
language |
English |
description |
Migratory birds may have a vital role in the spread of antimicrobial resistance across habitats and regions, but empirical data remain scarce. We investigated differences in the gut microbiome composition and the abundance of antibiotic resistance genes (ARGs) in faeces from four migratory waterbirds wintering in South-West Spain that differ in their habitat use. The white stork Ciconia ciconia and lesser black-backed gull Larus fuscus are omnivorous and opportunistic birds that use highly anthropogenic habitats such as landfills and urban areas. The greylag goose Anser anser and common crane Grus grus are herbivores and use more natural habitats. Fresh faeces from 15 individuals of each species were analysed to assess the composition of bacterial communities using 16S rRNA amplicon-targeted sequencing, and to quantify the abundance of the Class I integron integrase gene (intI1) as well as genes encoding resistance to sulfonamides (sul1), beta-lactams (blaTEM, blaKPC and blaNDM), tetracyclines (tetW), fluoroquinolones (qnrS), and colistin (mcr-1) using qPCR. Bacterial communities in gull faeces were the richest and most diverse. Beta diversity analysis showed segregation in faecal communities between bird species, but those from storks and gulls were the most similar, these being the species that regularly feed in landfills. Potential bacterial pathogens identified in faeces differed significantly between bird species, with higher relative abundance in gulls. Faeces from birds that feed in landfills (stork and gull) contained a significantly higher abundance of ARGs (sul1, blaTEM, and tetW). Genes conferring resistance to last resort antibiotics such as carbapenems (blaKPC) and colistin (mcr-1) were only observed in faeces from gulls. These results show that these bird species are reservoirs of antimicrobial resistant bacteria and suggest that waterbirds may disseminate antibiotic resistance across environments (e.g., from landfills to ricefields or water supplies), and thus constitute a risk for their further ... |
author2 |
Biología |
format |
Article in Journal/Newspaper |
author |
Jarma, Dayana Ayelen Sánchez, Marta I. Green, Andy J. Peralta-Sánchez, Juan Manuel Hortas Rodríguez-Pascual, Francisco Sánchez-Melsió, Alexandre Borrego, Carles M. |
spellingShingle |
Jarma, Dayana Ayelen Sánchez, Marta I. Green, Andy J. Peralta-Sánchez, Juan Manuel Hortas Rodríguez-Pascual, Francisco Sánchez-Melsió, Alexandre Borrego, Carles M. Faecal microbiota and antibiotic resistance genes in migratory waterbirds with contrasting habitat use. |
author_facet |
Jarma, Dayana Ayelen Sánchez, Marta I. Green, Andy J. Peralta-Sánchez, Juan Manuel Hortas Rodríguez-Pascual, Francisco Sánchez-Melsió, Alexandre Borrego, Carles M. |
author_sort |
Jarma, Dayana Ayelen |
title |
Faecal microbiota and antibiotic resistance genes in migratory waterbirds with contrasting habitat use. |
title_short |
Faecal microbiota and antibiotic resistance genes in migratory waterbirds with contrasting habitat use. |
title_full |
Faecal microbiota and antibiotic resistance genes in migratory waterbirds with contrasting habitat use. |
title_fullStr |
Faecal microbiota and antibiotic resistance genes in migratory waterbirds with contrasting habitat use. |
title_full_unstemmed |
Faecal microbiota and antibiotic resistance genes in migratory waterbirds with contrasting habitat use. |
title_sort |
faecal microbiota and antibiotic resistance genes in migratory waterbirds with contrasting habitat use. |
publisher |
ELSEVIER |
publishDate |
2021 |
url |
http://hdl.handle.net/10498/24955 https://doi.org/10.1016/j.scitotenv.2021.146872 |
genre |
Lesser black-backed gull |
genre_facet |
Lesser black-backed gull |
op_source |
Science of the Total Environment 783 (2021) 146872 |
op_relation |
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108962GB-C21/ES/AVES ACUATICAS COMO VECTORES DE DISPERSION DE RESISTENCIAS: PAPEL DE LA ECOLOGIA Y LA CONTAMINACION AMBIENTAL/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108962GB-C22/ES/AVES ACUATICAS COMO RESERVORIOS DE RESISTENCIAS A ANTIBIOTICOS/ 1879-1026 http://hdl.handle.net/10498/24955 doi:10.1016/j.scitotenv.2021.146872 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.scitotenv.2021.146872 |
container_title |
Science of The Total Environment |
container_volume |
783 |
container_start_page |
146872 |
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1766063457598177280 |