Advancing methodology for sub-zero temperature application of DGT technique on sea ice samples for two-dimensional imaging of biogenic metals
Ice-associated communities colonize the brine-filled spaces and are exposed to major biogeochemical and physical changes: temperature fluctuations, salinity, dissolved oxygen, light, pH, the surrounding organic matrix, and nutrient stress. A key adaptive response is the formation of biofilms, which...
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ftunivbruxelles:oai:dipot.ulb.ac.be:2013/299540 2023-05-15T16:39:24+02:00 Advancing methodology for sub-zero temperature application of DGT technique on sea ice samples for two-dimensional imaging of biogenic metals Bratkic, Arne Zhou, C Tison, Jean-Louis Fripiat, François Gao, Yue Delille, B. 6th Conference on Diffusive Gradients in Thin Films 2019 (2019: Vienna) 2019-09-18 No full-text files http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/299540 fr fre http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/299540 Sciences de la terre et du cosmos info:eu-repo/semantics/conferenceContribution info:ulb-repo/semantics/conferenceContribution info:ulb-repo/semantics/openurl/document 2019 ftunivbruxelles 2022-06-12T21:55:54Z Ice-associated communities colonize the brine-filled spaces and are exposed to major biogeochemical and physical changes: temperature fluctuations, salinity, dissolved oxygen, light, pH, the surrounding organic matrix, and nutrient stress. A key adaptive response is the formation of biofilms, which play a major role in macro- and micro-nutrient storage, transformation and mobilization. Considerable enrichment of Fe and other trace metals has been recorded in sea ice, supposedly being adsorbed onto organic matter. Current methods for collecting pristine ice samples mostly involve melting an ice core, erasing any spatial information and discrimination between solid, liquid and gaseous phases. As a result, sea ice analytical methods have an insufficient spatial resolution to detect or describe microbial processes at submillimetre scale (in biofilms within the brine network), without any existing alternative. We have developed a new Diffusive Gradients in Thin-films (DGT) procedure for sea ice application, based on DGT capacity for imaging 2-dimensional distribution of total labile metal concentrations in soil/sediment. During the optimization process, we considered atypical conditions for DGT application at sub-zero temperatures; hydrogel freezing, slow diffusion, high brine salinity. We defined diffusive coefficients at water freezing temperatures and assured contact with hydrogel and thus diffusion. Using Peltier element to precisely control ambient temperature, slow equilibration to in situ temperature of -1.8°C successfully maintained the brine liquid, ice remained solid, and the hydrogel did not freeze. This allowed diffusion to occur, and importantly, allowed sea ice to de-gas. Without gradual equilibration, gases from sea ice were trapped between hydrogel and ice, separating the two and preventing diffusion. Our result are the first two-dimensional images of biogenic metal micronutrients in the sea ice, revealing a clear spatially diverse signal. Fe, Zn and Mn were associated with organic matter-rich ... Conference Object ice core Sea ice DI-fusion : dépôt institutionnel de l'Université libre de Bruxelles (ULB) Peltier ENVELOPE(-63.495,-63.495,-64.854,-64.854) |
institution |
Open Polar |
collection |
DI-fusion : dépôt institutionnel de l'Université libre de Bruxelles (ULB) |
op_collection_id |
ftunivbruxelles |
language |
French |
topic |
Sciences de la terre et du cosmos |
spellingShingle |
Sciences de la terre et du cosmos Bratkic, Arne Zhou, C Tison, Jean-Louis Fripiat, François Gao, Yue Delille, B. Advancing methodology for sub-zero temperature application of DGT technique on sea ice samples for two-dimensional imaging of biogenic metals |
topic_facet |
Sciences de la terre et du cosmos |
description |
Ice-associated communities colonize the brine-filled spaces and are exposed to major biogeochemical and physical changes: temperature fluctuations, salinity, dissolved oxygen, light, pH, the surrounding organic matrix, and nutrient stress. A key adaptive response is the formation of biofilms, which play a major role in macro- and micro-nutrient storage, transformation and mobilization. Considerable enrichment of Fe and other trace metals has been recorded in sea ice, supposedly being adsorbed onto organic matter. Current methods for collecting pristine ice samples mostly involve melting an ice core, erasing any spatial information and discrimination between solid, liquid and gaseous phases. As a result, sea ice analytical methods have an insufficient spatial resolution to detect or describe microbial processes at submillimetre scale (in biofilms within the brine network), without any existing alternative. We have developed a new Diffusive Gradients in Thin-films (DGT) procedure for sea ice application, based on DGT capacity for imaging 2-dimensional distribution of total labile metal concentrations in soil/sediment. During the optimization process, we considered atypical conditions for DGT application at sub-zero temperatures; hydrogel freezing, slow diffusion, high brine salinity. We defined diffusive coefficients at water freezing temperatures and assured contact with hydrogel and thus diffusion. Using Peltier element to precisely control ambient temperature, slow equilibration to in situ temperature of -1.8°C successfully maintained the brine liquid, ice remained solid, and the hydrogel did not freeze. This allowed diffusion to occur, and importantly, allowed sea ice to de-gas. Without gradual equilibration, gases from sea ice were trapped between hydrogel and ice, separating the two and preventing diffusion. Our result are the first two-dimensional images of biogenic metal micronutrients in the sea ice, revealing a clear spatially diverse signal. Fe, Zn and Mn were associated with organic matter-rich ... |
author2 |
6th Conference on Diffusive Gradients in Thin Films 2019 (2019: Vienna) |
format |
Conference Object |
author |
Bratkic, Arne Zhou, C Tison, Jean-Louis Fripiat, François Gao, Yue Delille, B. |
author_facet |
Bratkic, Arne Zhou, C Tison, Jean-Louis Fripiat, François Gao, Yue Delille, B. |
author_sort |
Bratkic, Arne |
title |
Advancing methodology for sub-zero temperature application of DGT technique on sea ice samples for two-dimensional imaging of biogenic metals |
title_short |
Advancing methodology for sub-zero temperature application of DGT technique on sea ice samples for two-dimensional imaging of biogenic metals |
title_full |
Advancing methodology for sub-zero temperature application of DGT technique on sea ice samples for two-dimensional imaging of biogenic metals |
title_fullStr |
Advancing methodology for sub-zero temperature application of DGT technique on sea ice samples for two-dimensional imaging of biogenic metals |
title_full_unstemmed |
Advancing methodology for sub-zero temperature application of DGT technique on sea ice samples for two-dimensional imaging of biogenic metals |
title_sort |
advancing methodology for sub-zero temperature application of dgt technique on sea ice samples for two-dimensional imaging of biogenic metals |
publishDate |
2019 |
url |
http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/299540 |
long_lat |
ENVELOPE(-63.495,-63.495,-64.854,-64.854) |
geographic |
Peltier |
geographic_facet |
Peltier |
genre |
ice core Sea ice |
genre_facet |
ice core Sea ice |
op_relation |
http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/299540 |
_version_ |
1766029749151334400 |