The first known virus isolates from Antarctic sea ice have complex infection patterns
Viruses are recognized as important actors in ocean ecology and biogeochemical cycles, but many details are not yet understood. We participated in a winter expedition to the Weddell Sea, Antarctica, to isolate viruses and to measure virus-like particle abundance (flow cytometry) in sea ice. We isola...
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ftunivbruxelles:oai:dipot.ulb.ac.be:2013/269441 2023-05-15T13:51:43+02:00 The first known virus isolates from Antarctic sea ice have complex infection patterns Luhtanen, Anne Mari Eronen-Rasimus, Eeva Oksanen, Hanna H.M. Tison, Jean-Louis Delille, Bruno Dieckmann, Gerhard Rintala, Janne Markus Bamford, Dennis D.H. 2018-04 1 full-text file(s): application/pdf http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/269441 https://dipot.ulb.ac.be/dspace/bitstream/2013/269441/1/doi_253068.pdf en eng uri/info:doi/10.1093/femsec/fiy028 uri/info:scp/85043791422 https://dipot.ulb.ac.be/dspace/bitstream/2013/269441/1/doi_253068.pdf http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/269441 1 full-text file(s): info:eu-repo/semantics/restrictedAccess FEMS microbiology, ecology, 94 (4 Ecologie Microbiologie et protistologie [bacteriol.virolog.mycolog.] Biotechnologie Sea ice microbiology Virus isolation Virus-host interactions VLP in sea ice info:eu-repo/semantics/article info:ulb-repo/semantics/articlePeerReview info:ulb-repo/semantics/openurl/article 2018 ftunivbruxelles 2022-06-12T21:41:53Z Viruses are recognized as important actors in ocean ecology and biogeochemical cycles, but many details are not yet understood. We participated in a winter expedition to the Weddell Sea, Antarctica, to isolate viruses and to measure virus-like particle abundance (flow cytometry) in sea ice. We isolated 59 bacterial strains and the first four Antarctic sea-ice viruses known (PANV1, PANV2, OANV1 and OANV2), which grow in bacterial hosts belonging to the typical sea-ice genera Paraglaciecola and Octadecabacter. The viruses were specific for bacteria at the strain level, although OANV1 was able to infect strains from two different classes. Both PANV1 and PANV2 infected 11/15 isolated Paraglaciecola strains that had almost identical 16S rRNA gene sequences, but the plating efficiencies differed among the strains, whereas OANV1 infected 3/7 Octadecabacter and 1/15 Paraglaciecola strains and OANV2 1/7 Octadecabacter strains. All the phages were cold-active and able to infect their original host at 0°C and 4°C, but not at higher temperatures. The results showed that virus-host interactions can be very complex and that the viral community can also be dynamic in the winter-sea ice. SCOPUS: ar.j info:eu-repo/semantics/published Article in Journal/Newspaper Antarc* Antarctic Antarctica Sea ice Weddell Sea DI-fusion : dépôt institutionnel de l'Université libre de Bruxelles (ULB) Antarctic Weddell Sea Weddell |
institution |
Open Polar |
collection |
DI-fusion : dépôt institutionnel de l'Université libre de Bruxelles (ULB) |
op_collection_id |
ftunivbruxelles |
language |
English |
topic |
Ecologie Microbiologie et protistologie [bacteriol.virolog.mycolog.] Biotechnologie Sea ice microbiology Virus isolation Virus-host interactions VLP in sea ice |
spellingShingle |
Ecologie Microbiologie et protistologie [bacteriol.virolog.mycolog.] Biotechnologie Sea ice microbiology Virus isolation Virus-host interactions VLP in sea ice Luhtanen, Anne Mari Eronen-Rasimus, Eeva Oksanen, Hanna H.M. Tison, Jean-Louis Delille, Bruno Dieckmann, Gerhard Rintala, Janne Markus Bamford, Dennis D.H. The first known virus isolates from Antarctic sea ice have complex infection patterns |
topic_facet |
Ecologie Microbiologie et protistologie [bacteriol.virolog.mycolog.] Biotechnologie Sea ice microbiology Virus isolation Virus-host interactions VLP in sea ice |
description |
Viruses are recognized as important actors in ocean ecology and biogeochemical cycles, but many details are not yet understood. We participated in a winter expedition to the Weddell Sea, Antarctica, to isolate viruses and to measure virus-like particle abundance (flow cytometry) in sea ice. We isolated 59 bacterial strains and the first four Antarctic sea-ice viruses known (PANV1, PANV2, OANV1 and OANV2), which grow in bacterial hosts belonging to the typical sea-ice genera Paraglaciecola and Octadecabacter. The viruses were specific for bacteria at the strain level, although OANV1 was able to infect strains from two different classes. Both PANV1 and PANV2 infected 11/15 isolated Paraglaciecola strains that had almost identical 16S rRNA gene sequences, but the plating efficiencies differed among the strains, whereas OANV1 infected 3/7 Octadecabacter and 1/15 Paraglaciecola strains and OANV2 1/7 Octadecabacter strains. All the phages were cold-active and able to infect their original host at 0°C and 4°C, but not at higher temperatures. The results showed that virus-host interactions can be very complex and that the viral community can also be dynamic in the winter-sea ice. SCOPUS: ar.j info:eu-repo/semantics/published |
format |
Article in Journal/Newspaper |
author |
Luhtanen, Anne Mari Eronen-Rasimus, Eeva Oksanen, Hanna H.M. Tison, Jean-Louis Delille, Bruno Dieckmann, Gerhard Rintala, Janne Markus Bamford, Dennis D.H. |
author_facet |
Luhtanen, Anne Mari Eronen-Rasimus, Eeva Oksanen, Hanna H.M. Tison, Jean-Louis Delille, Bruno Dieckmann, Gerhard Rintala, Janne Markus Bamford, Dennis D.H. |
author_sort |
Luhtanen, Anne Mari |
title |
The first known virus isolates from Antarctic sea ice have complex infection patterns |
title_short |
The first known virus isolates from Antarctic sea ice have complex infection patterns |
title_full |
The first known virus isolates from Antarctic sea ice have complex infection patterns |
title_fullStr |
The first known virus isolates from Antarctic sea ice have complex infection patterns |
title_full_unstemmed |
The first known virus isolates from Antarctic sea ice have complex infection patterns |
title_sort |
first known virus isolates from antarctic sea ice have complex infection patterns |
publishDate |
2018 |
url |
http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/269441 https://dipot.ulb.ac.be/dspace/bitstream/2013/269441/1/doi_253068.pdf |
geographic |
Antarctic Weddell Sea Weddell |
geographic_facet |
Antarctic Weddell Sea Weddell |
genre |
Antarc* Antarctic Antarctica Sea ice Weddell Sea |
genre_facet |
Antarc* Antarctic Antarctica Sea ice Weddell Sea |
op_source |
FEMS microbiology, ecology, 94 (4 |
op_relation |
uri/info:doi/10.1093/femsec/fiy028 uri/info:scp/85043791422 https://dipot.ulb.ac.be/dspace/bitstream/2013/269441/1/doi_253068.pdf http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/269441 |
op_rights |
1 full-text file(s): info:eu-repo/semantics/restrictedAccess |
_version_ |
1766255746135097344 |