The basic helix-loop-helix transcription factor CrMYC2 controls the jasmonate-responsive expression of the ORCA genes that regulate alkaloid biosynthesis in Catharanthus roseus.
Jasmonates are plant signalling molecules that play key roles in defence against insects and certain pathogens, among others by controlling the biosynthesis of protective secondary metabolites. In Catharanthus roseus, the AP2/ERF-domain transcription factor ORCA3 controls the jasmonate-responsive ex...
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ftunivbruxelles:oai:dipot.ulb.ac.be:2013/143941 2023-05-15T17:53:45+02:00 The basic helix-loop-helix transcription factor CrMYC2 controls the jasmonate-responsive expression of the ORCA genes that regulate alkaloid biosynthesis in Catharanthus roseus. Zhang, Hongtao Hedhili, Sabah Montiel, Grégory Zhang, Yanxia Chatel, Guillaume Pré, Martial Gantet, Pascal Memelink, Johan 2011-07 1 full-text file(s): application/pdf http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/143941 https://dipot.ulb.ac.be/dspace/bitstream/2013/143941/3/143941.pdf en eng uri/info:doi/10.1111/j.1365-313X.2011.04575.x uri/info:pmid/21401746 https://dipot.ulb.ac.be/dspace/bitstream/2013/143941/3/143941.pdf http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/143941 1 full-text file(s): info:eu-repo/semantics/restrictedAccess Plant journal, 67 (1 Sciences bio-médicales et agricoles Acetates -- pharmacology Alkaloids -- analysis -- biosynthesis Basic Helix-Loop-Helix Transcription Factors -- genetics -- metabolism Catharanthus -- genetics -- metabolism Cell Line Cyclopentanes -- pharmacology DNA Complementary Gene Expression Gene Expression Regulation Plant Genes Reporter Oxylipins -- pharmacology Plant Growth Regulators -- pharmacology Plant Proteins -- genetics -- metabolism Promoter Regions Genetic RNA Interference RNA Messenger -- genetics Plant -- genetics Recombinant Proteins Trans-Activators -- genetics -- metabolism Transcription Factors -- genetics -- metabolism Transcriptional Activation info:eu-repo/semantics/article info:ulb-repo/semantics/articlePeerReview info:ulb-repo/semantics/openurl/article 2011 ftunivbruxelles 2022-06-12T21:23:42Z Jasmonates are plant signalling molecules that play key roles in defence against insects and certain pathogens, among others by controlling the biosynthesis of protective secondary metabolites. In Catharanthus roseus, the AP2/ERF-domain transcription factor ORCA3 controls the jasmonate-responsive expression of several genes encoding enzymes involved in terpenoid indole alkaloid biosynthesis. ORCA3 gene expression is itself induced by jasmonate. The ORCA3 promoter contains an autonomous jasmonate-responsive element (JRE) composed of a quantitative sequence responsible for the high level of expression and a qualitative sequence that acts as an on/off switch in response to methyl-jasmonate (MeJA). Here, we identify the basic helix-loop-helix (bHLH) transcription factor CrMYC2 as the major activator of MeJA-responsive ORCA3 gene expression. The CrMYC2 gene is an immediate-early jasmonate-responsive gene. CrMYC2 binds to the qualitative sequence in the ORCA3 JRE in vitro, and transactivates reporter gene expression via this sequence in transient assays. Knock-down of the CrMYC2 expression level via RNA interference caused a strong reduction in the level of MeJA-responsive ORCA3 mRNA accumulation. In addition, MeJA-responsive expression of the related transcription factor gene ORCA2 was significantly reduced. Our results show that MeJA-responsive expression of alkaloid biosynthesis genes in C. roseus is controlled by a transcription factor cascade consisting of the bHLH protein CrMYC2 regulating ORCA gene expression, and the AP2/ERF-domain transcription factors ORCA2 and ORCA3, which in turn regulate a subset of alkaloid biosynthesis genes. Journal Article Research Support, Non-U.S. Gov't FLWIN info:eu-repo/semantics/published Article in Journal/Newspaper Orca DI-fusion : dépôt institutionnel de l'Université libre de Bruxelles (ULB) |
institution |
Open Polar |
collection |
DI-fusion : dépôt institutionnel de l'Université libre de Bruxelles (ULB) |
op_collection_id |
ftunivbruxelles |
language |
English |
topic |
Sciences bio-médicales et agricoles Acetates -- pharmacology Alkaloids -- analysis -- biosynthesis Basic Helix-Loop-Helix Transcription Factors -- genetics -- metabolism Catharanthus -- genetics -- metabolism Cell Line Cyclopentanes -- pharmacology DNA Complementary Gene Expression Gene Expression Regulation Plant Genes Reporter Oxylipins -- pharmacology Plant Growth Regulators -- pharmacology Plant Proteins -- genetics -- metabolism Promoter Regions Genetic RNA Interference RNA Messenger -- genetics Plant -- genetics Recombinant Proteins Trans-Activators -- genetics -- metabolism Transcription Factors -- genetics -- metabolism Transcriptional Activation |
spellingShingle |
Sciences bio-médicales et agricoles Acetates -- pharmacology Alkaloids -- analysis -- biosynthesis Basic Helix-Loop-Helix Transcription Factors -- genetics -- metabolism Catharanthus -- genetics -- metabolism Cell Line Cyclopentanes -- pharmacology DNA Complementary Gene Expression Gene Expression Regulation Plant Genes Reporter Oxylipins -- pharmacology Plant Growth Regulators -- pharmacology Plant Proteins -- genetics -- metabolism Promoter Regions Genetic RNA Interference RNA Messenger -- genetics Plant -- genetics Recombinant Proteins Trans-Activators -- genetics -- metabolism Transcription Factors -- genetics -- metabolism Transcriptional Activation Zhang, Hongtao Hedhili, Sabah Montiel, Grégory Zhang, Yanxia Chatel, Guillaume Pré, Martial Gantet, Pascal Memelink, Johan The basic helix-loop-helix transcription factor CrMYC2 controls the jasmonate-responsive expression of the ORCA genes that regulate alkaloid biosynthesis in Catharanthus roseus. |
topic_facet |
Sciences bio-médicales et agricoles Acetates -- pharmacology Alkaloids -- analysis -- biosynthesis Basic Helix-Loop-Helix Transcription Factors -- genetics -- metabolism Catharanthus -- genetics -- metabolism Cell Line Cyclopentanes -- pharmacology DNA Complementary Gene Expression Gene Expression Regulation Plant Genes Reporter Oxylipins -- pharmacology Plant Growth Regulators -- pharmacology Plant Proteins -- genetics -- metabolism Promoter Regions Genetic RNA Interference RNA Messenger -- genetics Plant -- genetics Recombinant Proteins Trans-Activators -- genetics -- metabolism Transcription Factors -- genetics -- metabolism Transcriptional Activation |
description |
Jasmonates are plant signalling molecules that play key roles in defence against insects and certain pathogens, among others by controlling the biosynthesis of protective secondary metabolites. In Catharanthus roseus, the AP2/ERF-domain transcription factor ORCA3 controls the jasmonate-responsive expression of several genes encoding enzymes involved in terpenoid indole alkaloid biosynthesis. ORCA3 gene expression is itself induced by jasmonate. The ORCA3 promoter contains an autonomous jasmonate-responsive element (JRE) composed of a quantitative sequence responsible for the high level of expression and a qualitative sequence that acts as an on/off switch in response to methyl-jasmonate (MeJA). Here, we identify the basic helix-loop-helix (bHLH) transcription factor CrMYC2 as the major activator of MeJA-responsive ORCA3 gene expression. The CrMYC2 gene is an immediate-early jasmonate-responsive gene. CrMYC2 binds to the qualitative sequence in the ORCA3 JRE in vitro, and transactivates reporter gene expression via this sequence in transient assays. Knock-down of the CrMYC2 expression level via RNA interference caused a strong reduction in the level of MeJA-responsive ORCA3 mRNA accumulation. In addition, MeJA-responsive expression of the related transcription factor gene ORCA2 was significantly reduced. Our results show that MeJA-responsive expression of alkaloid biosynthesis genes in C. roseus is controlled by a transcription factor cascade consisting of the bHLH protein CrMYC2 regulating ORCA gene expression, and the AP2/ERF-domain transcription factors ORCA2 and ORCA3, which in turn regulate a subset of alkaloid biosynthesis genes. Journal Article Research Support, Non-U.S. Gov't FLWIN info:eu-repo/semantics/published |
format |
Article in Journal/Newspaper |
author |
Zhang, Hongtao Hedhili, Sabah Montiel, Grégory Zhang, Yanxia Chatel, Guillaume Pré, Martial Gantet, Pascal Memelink, Johan |
author_facet |
Zhang, Hongtao Hedhili, Sabah Montiel, Grégory Zhang, Yanxia Chatel, Guillaume Pré, Martial Gantet, Pascal Memelink, Johan |
author_sort |
Zhang, Hongtao |
title |
The basic helix-loop-helix transcription factor CrMYC2 controls the jasmonate-responsive expression of the ORCA genes that regulate alkaloid biosynthesis in Catharanthus roseus. |
title_short |
The basic helix-loop-helix transcription factor CrMYC2 controls the jasmonate-responsive expression of the ORCA genes that regulate alkaloid biosynthesis in Catharanthus roseus. |
title_full |
The basic helix-loop-helix transcription factor CrMYC2 controls the jasmonate-responsive expression of the ORCA genes that regulate alkaloid biosynthesis in Catharanthus roseus. |
title_fullStr |
The basic helix-loop-helix transcription factor CrMYC2 controls the jasmonate-responsive expression of the ORCA genes that regulate alkaloid biosynthesis in Catharanthus roseus. |
title_full_unstemmed |
The basic helix-loop-helix transcription factor CrMYC2 controls the jasmonate-responsive expression of the ORCA genes that regulate alkaloid biosynthesis in Catharanthus roseus. |
title_sort |
basic helix-loop-helix transcription factor crmyc2 controls the jasmonate-responsive expression of the orca genes that regulate alkaloid biosynthesis in catharanthus roseus. |
publishDate |
2011 |
url |
http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/143941 https://dipot.ulb.ac.be/dspace/bitstream/2013/143941/3/143941.pdf |
genre |
Orca |
genre_facet |
Orca |
op_source |
Plant journal, 67 (1 |
op_relation |
uri/info:doi/10.1111/j.1365-313X.2011.04575.x uri/info:pmid/21401746 https://dipot.ulb.ac.be/dspace/bitstream/2013/143941/3/143941.pdf http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/143941 |
op_rights |
1 full-text file(s): info:eu-repo/semantics/restrictedAccess |
_version_ |
1766161459683786752 |