A robust approach for the determination of dimethylsulfoxide in sea ice
The melting of sea ice samples is acknowledged to be deleterious to sympagic microorganisms due to the hypo-osmotic shock undergone by the organism when released from high salinity brine inclusions into the sample melt. Because melting of sea ice samples was also anticipated to modify the initial pr...
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ftunivbruxelles:oai:dipot.ulb.ac.be:2013/129580 2023-05-15T13:53:32+02:00 A robust approach for the determination of dimethylsulfoxide in sea ice Brabant, Frédéric El Amri, Saïda Tison, Jean-Louis 2011 1 full-text file(s): application/pdf http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/129580 https://dipot.ulb.ac.be/dspace/bitstream/2013/129580/3/129580.pdf en eng uri/info:doi/10.4319/lom.2011.9.261 uri/info:scp/79960463940 https://dipot.ulb.ac.be/dspace/bitstream/2013/129580/3/129580.pdf http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/129580 1 full-text file(s): info:eu-repo/semantics/restrictedAccess Limnology and oceanography, methods, 9 Glaciologie Climatologie Géographie physique Océanographie physique et chimique info:eu-repo/semantics/article info:ulb-repo/semantics/articlePeerReview info:ulb-repo/semantics/openurl/article 2011 ftunivbruxelles 2022-06-12T21:18:18Z The melting of sea ice samples is acknowledged to be deleterious to sympagic microorganisms due to the hypo-osmotic shock undergone by the organism when released from high salinity brine inclusions into the sample melt. Because melting of sea ice samples was also anticipated to modify the initial proportions of dimethylsulfide (DMS), dimethylsulfoniopropionate (DMSP), and dimethylsulfoxide (DMSO), three sample treatments were tested on an Antarctic sea ice sample, with the aim of identifying an efficient procedure that could routinely be applied for the determination of DMSO in sea ice. Herein, it was demonstrated that purging the melted sample before the determination of DMSO in the sample via an enzyme-linked method produced reliable DMSO results (215.8 ± 8.9 nmol L -1, precision 4.1%). However, analysis revealed that the unintentional enzymatic cleavage of DMSP through the subsequent production of interfering DMS during melting caused an overestimation of the DMSO content in the sample by more than 59% and concurrently an underestimation of the DMSP content by approximately 9%. The sequential determination of DMSP after the DMSO determination by the enzyme-linked method was shown to be problematic. To circumvent all of these issues, we recommend an analytical procedure for the sequential determination of DMS, DMSP, and DMSO in sea ice. Ultimately, the first depth profile of DMSO at high resolution in sea ice was produced. The depth-integrated DMSO concentration in sea ice was determined to be 718 μmol m -2, which indicated that Antarctic sea ice is a potentially important source of DMSO for the Southern Ocean. © 2011, by the American Society of Limnology and Oceanography, Inc. SCOPUS: ar.j FLWIN info:eu-repo/semantics/published Article in Journal/Newspaper Antarc* Antarctic Sea ice Southern Ocean DI-fusion : dépôt institutionnel de l'Université libre de Bruxelles (ULB) Antarctic Southern Ocean |
institution |
Open Polar |
collection |
DI-fusion : dépôt institutionnel de l'Université libre de Bruxelles (ULB) |
op_collection_id |
ftunivbruxelles |
language |
English |
topic |
Glaciologie Climatologie Géographie physique Océanographie physique et chimique |
spellingShingle |
Glaciologie Climatologie Géographie physique Océanographie physique et chimique Brabant, Frédéric El Amri, Saïda Tison, Jean-Louis A robust approach for the determination of dimethylsulfoxide in sea ice |
topic_facet |
Glaciologie Climatologie Géographie physique Océanographie physique et chimique |
description |
The melting of sea ice samples is acknowledged to be deleterious to sympagic microorganisms due to the hypo-osmotic shock undergone by the organism when released from high salinity brine inclusions into the sample melt. Because melting of sea ice samples was also anticipated to modify the initial proportions of dimethylsulfide (DMS), dimethylsulfoniopropionate (DMSP), and dimethylsulfoxide (DMSO), three sample treatments were tested on an Antarctic sea ice sample, with the aim of identifying an efficient procedure that could routinely be applied for the determination of DMSO in sea ice. Herein, it was demonstrated that purging the melted sample before the determination of DMSO in the sample via an enzyme-linked method produced reliable DMSO results (215.8 ± 8.9 nmol L -1, precision 4.1%). However, analysis revealed that the unintentional enzymatic cleavage of DMSP through the subsequent production of interfering DMS during melting caused an overestimation of the DMSO content in the sample by more than 59% and concurrently an underestimation of the DMSP content by approximately 9%. The sequential determination of DMSP after the DMSO determination by the enzyme-linked method was shown to be problematic. To circumvent all of these issues, we recommend an analytical procedure for the sequential determination of DMS, DMSP, and DMSO in sea ice. Ultimately, the first depth profile of DMSO at high resolution in sea ice was produced. The depth-integrated DMSO concentration in sea ice was determined to be 718 μmol m -2, which indicated that Antarctic sea ice is a potentially important source of DMSO for the Southern Ocean. © 2011, by the American Society of Limnology and Oceanography, Inc. SCOPUS: ar.j FLWIN info:eu-repo/semantics/published |
format |
Article in Journal/Newspaper |
author |
Brabant, Frédéric El Amri, Saïda Tison, Jean-Louis |
author_facet |
Brabant, Frédéric El Amri, Saïda Tison, Jean-Louis |
author_sort |
Brabant, Frédéric |
title |
A robust approach for the determination of dimethylsulfoxide in sea ice |
title_short |
A robust approach for the determination of dimethylsulfoxide in sea ice |
title_full |
A robust approach for the determination of dimethylsulfoxide in sea ice |
title_fullStr |
A robust approach for the determination of dimethylsulfoxide in sea ice |
title_full_unstemmed |
A robust approach for the determination of dimethylsulfoxide in sea ice |
title_sort |
robust approach for the determination of dimethylsulfoxide in sea ice |
publishDate |
2011 |
url |
http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/129580 https://dipot.ulb.ac.be/dspace/bitstream/2013/129580/3/129580.pdf |
geographic |
Antarctic Southern Ocean |
geographic_facet |
Antarctic Southern Ocean |
genre |
Antarc* Antarctic Sea ice Southern Ocean |
genre_facet |
Antarc* Antarctic Sea ice Southern Ocean |
op_source |
Limnology and oceanography, methods, 9 |
op_relation |
uri/info:doi/10.4319/lom.2011.9.261 uri/info:scp/79960463940 https://dipot.ulb.ac.be/dspace/bitstream/2013/129580/3/129580.pdf http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/129580 |
op_rights |
1 full-text file(s): info:eu-repo/semantics/restrictedAccess |
_version_ |
1766258717310844928 |