The salmon louse Lepeophtheirus salmonis (Caligidae) as a vector of Aeromonas salmonicida
The sea louse, Lepeophtheirus salmonis, has been hypothesized to be a vector of fish pathogens and previous studies have isolated viral and bacterial pathogens from L. salmonis parasitizing farmed salmon. To examine the potential transmission of A. salmonicida by preadult and adult L. salmonis via p...
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University of British Columbia
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ftunivbritcolcir:oai:circle.library.ubc.ca:2429/44770 2023-05-15T15:31:12+02:00 The salmon louse Lepeophtheirus salmonis (Caligidae) as a vector of Aeromonas salmonicida Novak, Colin William 2013 http://hdl.handle.net/2429/44770 eng eng University of British Columbia Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ CC-BY-NC-ND Text Thesis/Dissertation 2013 ftunivbritcolcir 2019-10-15T18:11:07Z The sea louse, Lepeophtheirus salmonis, has been hypothesized to be a vector of fish pathogens and previous studies have isolated viral and bacterial pathogens from L. salmonis parasitizing farmed salmon. To examine the potential transmission of A. salmonicida by preadult and adult L. salmonis via parasitism of Atlantic salmon (Salmo salar), an in vivo bacteria-parasite challenge model was tested. Two pathogen challenge trials were performed, in which sea lice from donor (Aeromonas salmonicida-injected) fish were allocated among recipient fish for 14 days. Three hypotheses were examined: (i.) L. salmonis can acquire A. salmonicida from donor fish via parasitism; (ii.) A. salmonicida-exposed sea lice can transmit the pathogen to recipient Atlantic salmon via parasitism and (iii.) L. salmonis and A. salmonicida infections can cause synergistic effects on host fish. Sea lice acquired A. salmonicida externally (Trial 1 and 2, 100%) and internally (Trial 1, 100%) from parasitizing donor fish. Trial 1 (~44g fish) demonstrated a successful transfer of bacteria from lice to salmon (mucus, 100%; kidney, 77.3%), with a decrease (t = 5.29, df = 6, p = 0.00186) in mean fish condition factor and 59.1% cumulative fish mortality. Conversely, there was no evidence of bacteria transfer, no fish mortality and no decrease in mean fish condition factor in Trial 2 (~155g fish). In addition, histological examination revealed widespread inflammatory responses in small salmon (~46 g) infected with A. salmonicida and sea lice. Thus, preadult and adult L. salmonis can acquire and transmit A. salmonicida to recipient fish via parasitism under experimental conditions. However, the following conditions of pathogen, environment and host are required: (i.) a large inoculum of A. salmonicida (10⁶ - 10⁷ colony forming units (CFU) mL-¹), (ii.) internal acquisition of bacteria by sea lice and, (iii.) young Atlantic salmon post-smolts (~44g) as hosts. Land and Food Systems, Faculty of Graduate Thesis Atlantic salmon Salmo salar University of British Columbia: cIRcle - UBC's Information Repository |
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University of British Columbia: cIRcle - UBC's Information Repository |
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ftunivbritcolcir |
language |
English |
description |
The sea louse, Lepeophtheirus salmonis, has been hypothesized to be a vector of fish pathogens and previous studies have isolated viral and bacterial pathogens from L. salmonis parasitizing farmed salmon. To examine the potential transmission of A. salmonicida by preadult and adult L. salmonis via parasitism of Atlantic salmon (Salmo salar), an in vivo bacteria-parasite challenge model was tested. Two pathogen challenge trials were performed, in which sea lice from donor (Aeromonas salmonicida-injected) fish were allocated among recipient fish for 14 days. Three hypotheses were examined: (i.) L. salmonis can acquire A. salmonicida from donor fish via parasitism; (ii.) A. salmonicida-exposed sea lice can transmit the pathogen to recipient Atlantic salmon via parasitism and (iii.) L. salmonis and A. salmonicida infections can cause synergistic effects on host fish. Sea lice acquired A. salmonicida externally (Trial 1 and 2, 100%) and internally (Trial 1, 100%) from parasitizing donor fish. Trial 1 (~44g fish) demonstrated a successful transfer of bacteria from lice to salmon (mucus, 100%; kidney, 77.3%), with a decrease (t = 5.29, df = 6, p = 0.00186) in mean fish condition factor and 59.1% cumulative fish mortality. Conversely, there was no evidence of bacteria transfer, no fish mortality and no decrease in mean fish condition factor in Trial 2 (~155g fish). In addition, histological examination revealed widespread inflammatory responses in small salmon (~46 g) infected with A. salmonicida and sea lice. Thus, preadult and adult L. salmonis can acquire and transmit A. salmonicida to recipient fish via parasitism under experimental conditions. However, the following conditions of pathogen, environment and host are required: (i.) a large inoculum of A. salmonicida (10⁶ - 10⁷ colony forming units (CFU) mL-¹), (ii.) internal acquisition of bacteria by sea lice and, (iii.) young Atlantic salmon post-smolts (~44g) as hosts. Land and Food Systems, Faculty of Graduate |
format |
Thesis |
author |
Novak, Colin William |
spellingShingle |
Novak, Colin William The salmon louse Lepeophtheirus salmonis (Caligidae) as a vector of Aeromonas salmonicida |
author_facet |
Novak, Colin William |
author_sort |
Novak, Colin William |
title |
The salmon louse Lepeophtheirus salmonis (Caligidae) as a vector of Aeromonas salmonicida |
title_short |
The salmon louse Lepeophtheirus salmonis (Caligidae) as a vector of Aeromonas salmonicida |
title_full |
The salmon louse Lepeophtheirus salmonis (Caligidae) as a vector of Aeromonas salmonicida |
title_fullStr |
The salmon louse Lepeophtheirus salmonis (Caligidae) as a vector of Aeromonas salmonicida |
title_full_unstemmed |
The salmon louse Lepeophtheirus salmonis (Caligidae) as a vector of Aeromonas salmonicida |
title_sort |
salmon louse lepeophtheirus salmonis (caligidae) as a vector of aeromonas salmonicida |
publisher |
University of British Columbia |
publishDate |
2013 |
url |
http://hdl.handle.net/2429/44770 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_rightsnorm |
CC-BY-NC-ND |
_version_ |
1766361696171982848 |