Microarray-Based Identification of Gonad Transcripts Differentially Expressed Between Lines of Pacific Oyster Selected to Be Resistant or Susceptible to Summer Mortality
Summer mortality of the Pacific oyster Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. Heredity appears to be a major factor determining the sensitivity of oysters to summer mortality, allowing resistant (R) and susceptible (S) lines to be...
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Online Access: | https://hal.science/hal-04498762 https://doi.org/10.1007/s10126-009-9227-9 |
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ftunivbrest:oai:HAL:hal-04498762v1 2024-04-28T08:16:36+00:00 Microarray-Based Identification of Gonad Transcripts Differentially Expressed Between Lines of Pacific Oyster Selected to Be Resistant or Susceptible to Summer Mortality Fleury, Elodie Moal, Jeanne Boulo, Viviane Daniel, Jean-Yves Mazurais, David Henaut, Alain Corporeau, Charlotte Boudry, Pierre Favrel, Pascal Huvet, Arnaud Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Unité Biologie des Organismes Marins Exploités (BOME) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) 2010-06 https://hal.science/hal-04498762 https://doi.org/10.1007/s10126-009-9227-9 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s10126-009-9227-9 hal-04498762 https://hal.science/hal-04498762 doi:10.1007/s10126-009-9227-9 ISSN: 1436-2228 EISSN: 1436-2236 Marine Biotechnology https://hal.science/hal-04498762 Marine Biotechnology, 2010, 12 (3), pp.326-339. ⟨10.1007/s10126-009-9227-9⟩ [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2010 ftunivbrest https://doi.org/10.1007/s10126-009-9227-9 2024-04-04T17:01:31Z Summer mortality of the Pacific oyster Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. Heredity appears to be a major factor determining the sensitivity of oysters to summer mortality, allowing resistant (R) and susceptible (S) lines to be produced. We conducted genome-wide expression profiling of R and S gonads during the 3-month period preceding a summer mortality event, using a cDNA microarray that we designed. ANOVA analysis revealed that 34 genes were differentially expressed between R and S lines on four dates preceding the mortality event. Annotation of some of these genes highlights reproduction and its allocation and antioxidant defenses as the main pathways that operate differentially between R and S lines. This transcriptional analysis provides new indications to define markers for quantitative trait loci searches and functional studies and evaluate the potential role of each gene in the resistance to summer mortality. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Bretagne Occidentale: HAL Marine Biotechnology 12 3 326 339 |
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Open Polar |
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Université de Bretagne Occidentale: HAL |
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ftunivbrest |
language |
English |
topic |
[SDV]Life Sciences [q-bio] |
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[SDV]Life Sciences [q-bio] Fleury, Elodie Moal, Jeanne Boulo, Viviane Daniel, Jean-Yves Mazurais, David Henaut, Alain Corporeau, Charlotte Boudry, Pierre Favrel, Pascal Huvet, Arnaud Microarray-Based Identification of Gonad Transcripts Differentially Expressed Between Lines of Pacific Oyster Selected to Be Resistant or Susceptible to Summer Mortality |
topic_facet |
[SDV]Life Sciences [q-bio] |
description |
Summer mortality of the Pacific oyster Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. Heredity appears to be a major factor determining the sensitivity of oysters to summer mortality, allowing resistant (R) and susceptible (S) lines to be produced. We conducted genome-wide expression profiling of R and S gonads during the 3-month period preceding a summer mortality event, using a cDNA microarray that we designed. ANOVA analysis revealed that 34 genes were differentially expressed between R and S lines on four dates preceding the mortality event. Annotation of some of these genes highlights reproduction and its allocation and antioxidant defenses as the main pathways that operate differentially between R and S lines. This transcriptional analysis provides new indications to define markers for quantitative trait loci searches and functional studies and evaluate the potential role of each gene in the resistance to summer mortality. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Unité Biologie des Organismes Marins Exploités (BOME) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
format |
Article in Journal/Newspaper |
author |
Fleury, Elodie Moal, Jeanne Boulo, Viviane Daniel, Jean-Yves Mazurais, David Henaut, Alain Corporeau, Charlotte Boudry, Pierre Favrel, Pascal Huvet, Arnaud |
author_facet |
Fleury, Elodie Moal, Jeanne Boulo, Viviane Daniel, Jean-Yves Mazurais, David Henaut, Alain Corporeau, Charlotte Boudry, Pierre Favrel, Pascal Huvet, Arnaud |
author_sort |
Fleury, Elodie |
title |
Microarray-Based Identification of Gonad Transcripts Differentially Expressed Between Lines of Pacific Oyster Selected to Be Resistant or Susceptible to Summer Mortality |
title_short |
Microarray-Based Identification of Gonad Transcripts Differentially Expressed Between Lines of Pacific Oyster Selected to Be Resistant or Susceptible to Summer Mortality |
title_full |
Microarray-Based Identification of Gonad Transcripts Differentially Expressed Between Lines of Pacific Oyster Selected to Be Resistant or Susceptible to Summer Mortality |
title_fullStr |
Microarray-Based Identification of Gonad Transcripts Differentially Expressed Between Lines of Pacific Oyster Selected to Be Resistant or Susceptible to Summer Mortality |
title_full_unstemmed |
Microarray-Based Identification of Gonad Transcripts Differentially Expressed Between Lines of Pacific Oyster Selected to Be Resistant or Susceptible to Summer Mortality |
title_sort |
microarray-based identification of gonad transcripts differentially expressed between lines of pacific oyster selected to be resistant or susceptible to summer mortality |
publisher |
HAL CCSD |
publishDate |
2010 |
url |
https://hal.science/hal-04498762 https://doi.org/10.1007/s10126-009-9227-9 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1436-2228 EISSN: 1436-2236 Marine Biotechnology https://hal.science/hal-04498762 Marine Biotechnology, 2010, 12 (3), pp.326-339. ⟨10.1007/s10126-009-9227-9⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10126-009-9227-9 hal-04498762 https://hal.science/hal-04498762 doi:10.1007/s10126-009-9227-9 |
op_doi |
https://doi.org/10.1007/s10126-009-9227-9 |
container_title |
Marine Biotechnology |
container_volume |
12 |
container_issue |
3 |
container_start_page |
326 |
op_container_end_page |
339 |
_version_ |
1797581666950053888 |