Comparative study on cellular and molecular responses in oyster sperm revealed different susceptibilities to cryopreservation
WOS:000445802900026 The Portuguese (Crassostrea angulata) and Pacific oyster (Crassostrea gigas), both from the genus Crassostrea, are two important species for production and conservation. Although they have common characteristics, different susceptibilities to rearing conditions have been describe...
Published in: | Aquaculture |
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Main Authors: | , , , , , |
Other Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2019
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Subjects: | |
Online Access: | https://hal.science/hal-02874958 https://doi.org/10.1016/j.aquaculture.2018.08.049 |
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ftunivbrest:oai:HAL:hal-02874958v1 |
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institution |
Open Polar |
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Université de Bretagne Occidentale: HAL |
op_collection_id |
ftunivbrest |
language |
English |
topic |
ACL Crassostrea gigas pacific oyster lipid-peroxidation gene-expression crassostrea-gigas angulata Crassostrea angulata dna-damage embryo development human spermatozoa messenger-rnas quality Sperm cryopreservation Sperm post-thaw analysis Standardization [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
ACL Crassostrea gigas pacific oyster lipid-peroxidation gene-expression crassostrea-gigas angulata Crassostrea angulata dna-damage embryo development human spermatozoa messenger-rnas quality Sperm cryopreservation Sperm post-thaw analysis Standardization [SDE.BE]Environmental Sciences/Biodiversity and Ecology Riesco, Marta F. Felix, Francisca Matias, Domitilia Joaquim, Sandra Suquet, Marc Cabrita, Elsa Comparative study on cellular and molecular responses in oyster sperm revealed different susceptibilities to cryopreservation |
topic_facet |
ACL Crassostrea gigas pacific oyster lipid-peroxidation gene-expression crassostrea-gigas angulata Crassostrea angulata dna-damage embryo development human spermatozoa messenger-rnas quality Sperm cryopreservation Sperm post-thaw analysis Standardization [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
WOS:000445802900026 The Portuguese (Crassostrea angulata) and Pacific oyster (Crassostrea gigas), both from the genus Crassostrea, are two important species for production and conservation. Although they have common characteristics, different susceptibilities to rearing conditions have been described in these species. Overall, in the case of C. angulata, only a few remaining populations are present in the south of Portugal and Spain. The preservation of genetic material from improved stocks or from the original population is crucial in oysters to prevent the potential impacts of epidemic diseases and natural disasters. Sperm cryopreservation in oysters has progressed in recent years. However many issues, such as protocol standardization, are still unsolved for the application of research results. In the present study a sperm cryopreservation protocol, previously published in C. angulata by our group, were analyzed in terms of cellular and molecular damage, in an effort to determine the most sensible parameters to standardize the cryopreservation protocols for both species. Different approaches in the analysis of sperm quality were performed for the first time in this genus to detect different susceptibilities between the two valuable species. Our results revealed that our previously published protocol containing 10% DMSO as cryoprotectant is more suitable comparing to 10% EG in both species. In addition, an integrative analysis was performed in both oyster species comparing all cellular parameters and C. gigas showed a higher susceptibility to cryopreservation using this optimized protocol. Moreover, higher susceptibility to transcript degradation was detected in C. gigas using this optimized cryopreservation protocol. This study highlights the importance of using different techniques and exhaustive analyses for selecting the most suitable cryopreservation protocol and its standardization, thus ensuring the total safety of the technique. |
author2 |
Centro de Ciências do Mar Faro (CCMAR) Universidade do Algarve (UAlg) Instituto Português de Investigação do Mar e da Atmosfera (IPMA) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Centre of Marine Sciences Faro (CCMAR) University of Algarve Portugal |
format |
Article in Journal/Newspaper |
author |
Riesco, Marta F. Felix, Francisca Matias, Domitilia Joaquim, Sandra Suquet, Marc Cabrita, Elsa |
author_facet |
Riesco, Marta F. Felix, Francisca Matias, Domitilia Joaquim, Sandra Suquet, Marc Cabrita, Elsa |
author_sort |
Riesco, Marta F. |
title |
Comparative study on cellular and molecular responses in oyster sperm revealed different susceptibilities to cryopreservation |
title_short |
Comparative study on cellular and molecular responses in oyster sperm revealed different susceptibilities to cryopreservation |
title_full |
Comparative study on cellular and molecular responses in oyster sperm revealed different susceptibilities to cryopreservation |
title_fullStr |
Comparative study on cellular and molecular responses in oyster sperm revealed different susceptibilities to cryopreservation |
title_full_unstemmed |
Comparative study on cellular and molecular responses in oyster sperm revealed different susceptibilities to cryopreservation |
title_sort |
comparative study on cellular and molecular responses in oyster sperm revealed different susceptibilities to cryopreservation |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://hal.science/hal-02874958 https://doi.org/10.1016/j.aquaculture.2018.08.049 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0044-8486 EISSN: 1873-5622 Aquaculture https://hal.science/hal-02874958 Aquaculture, 2019, 498, pp.223-229. ⟨10.1016/j.aquaculture.2018.08.049⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2018.08.049 hal-02874958 https://hal.science/hal-02874958 doi:10.1016/j.aquaculture.2018.08.049 |
op_doi |
https://doi.org/10.1016/j.aquaculture.2018.08.049 |
container_title |
Aquaculture |
container_volume |
498 |
container_start_page |
223 |
op_container_end_page |
229 |
_version_ |
1790599217715085312 |
spelling |
ftunivbrest:oai:HAL:hal-02874958v1 2024-02-11T10:03:04+01:00 Comparative study on cellular and molecular responses in oyster sperm revealed different susceptibilities to cryopreservation Riesco, Marta F. Felix, Francisca Matias, Domitilia Joaquim, Sandra Suquet, Marc Cabrita, Elsa Centro de Ciências do Mar Faro (CCMAR) Universidade do Algarve (UAlg) Instituto Português de Investigação do Mar e da Atmosfera (IPMA) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Centre of Marine Sciences Faro (CCMAR) University of Algarve Portugal 2019 https://hal.science/hal-02874958 https://doi.org/10.1016/j.aquaculture.2018.08.049 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2018.08.049 hal-02874958 https://hal.science/hal-02874958 doi:10.1016/j.aquaculture.2018.08.049 ISSN: 0044-8486 EISSN: 1873-5622 Aquaculture https://hal.science/hal-02874958 Aquaculture, 2019, 498, pp.223-229. ⟨10.1016/j.aquaculture.2018.08.049⟩ ACL Crassostrea gigas pacific oyster lipid-peroxidation gene-expression crassostrea-gigas angulata Crassostrea angulata dna-damage embryo development human spermatozoa messenger-rnas quality Sperm cryopreservation Sperm post-thaw analysis Standardization [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2019 ftunivbrest https://doi.org/10.1016/j.aquaculture.2018.08.049 2024-01-23T23:39:43Z WOS:000445802900026 The Portuguese (Crassostrea angulata) and Pacific oyster (Crassostrea gigas), both from the genus Crassostrea, are two important species for production and conservation. Although they have common characteristics, different susceptibilities to rearing conditions have been described in these species. Overall, in the case of C. angulata, only a few remaining populations are present in the south of Portugal and Spain. The preservation of genetic material from improved stocks or from the original population is crucial in oysters to prevent the potential impacts of epidemic diseases and natural disasters. Sperm cryopreservation in oysters has progressed in recent years. However many issues, such as protocol standardization, are still unsolved for the application of research results. In the present study a sperm cryopreservation protocol, previously published in C. angulata by our group, were analyzed in terms of cellular and molecular damage, in an effort to determine the most sensible parameters to standardize the cryopreservation protocols for both species. Different approaches in the analysis of sperm quality were performed for the first time in this genus to detect different susceptibilities between the two valuable species. Our results revealed that our previously published protocol containing 10% DMSO as cryoprotectant is more suitable comparing to 10% EG in both species. In addition, an integrative analysis was performed in both oyster species comparing all cellular parameters and C. gigas showed a higher susceptibility to cryopreservation using this optimized protocol. Moreover, higher susceptibility to transcript degradation was detected in C. gigas using this optimized cryopreservation protocol. This study highlights the importance of using different techniques and exhaustive analyses for selecting the most suitable cryopreservation protocol and its standardization, thus ensuring the total safety of the technique. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Bretagne Occidentale: HAL Pacific Aquaculture 498 223 229 |