Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level
WOS:000435090700050 International audience European eel (Anguilla anguilla) is a euryhaline species, that has adapted to cope with both, hyper- and hypo-osmotic environments. This study investigates the effect of salinity, from a morphological and molecular point of view on European eel larvae reare...
Published in: | PLOS ONE |
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Main Authors: | , , , , , , |
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Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2018
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Online Access: | https://hal.science/hal-02640324 https://hal.science/hal-02640324/document https://hal.science/hal-02640324/file/Politis_etal_POne_2018.pdf https://doi.org/10.1371/journal.pone.0198294 |
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Université de Bretagne Occidentale: HAL |
op_collection_id |
ftunivbrest |
language |
English |
topic |
ACL embryonic-development japanese eel early-life stages gene-expression water temperature anguilla-japonica artificial maturation cultured larvae thyroid-hormone receptors yolk resorption [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BA.ZV]Life Sciences [q-bio]/Animal biology/Vertebrate Zoology [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis |
spellingShingle |
ACL embryonic-development japanese eel early-life stages gene-expression water temperature anguilla-japonica artificial maturation cultured larvae thyroid-hormone receptors yolk resorption [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BA.ZV]Life Sciences [q-bio]/Animal biology/Vertebrate Zoology [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna J. Tomkiewicz, Jonna Butts, Ian A. E. Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level |
topic_facet |
ACL embryonic-development japanese eel early-life stages gene-expression water temperature anguilla-japonica artificial maturation cultured larvae thyroid-hormone receptors yolk resorption [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BA.ZV]Life Sciences [q-bio]/Animal biology/Vertebrate Zoology [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis |
description |
WOS:000435090700050 International audience European eel (Anguilla anguilla) is a euryhaline species, that has adapted to cope with both, hyper- and hypo-osmotic environments. This study investigates the effect of salinity, from a morphological and molecular point of view on European eel larvae reared from 0 to 12 days post hatch (dph). Offspring reared in 36 practical salinity units (psu; control), were compared with larvae reared in six scenarios, where salinity was decreased on 0 or 3 dph and in rates of 1, 2 or 4 psu/day, towards iso-osmotic conditions. Results showed that several genes relating to osmoregulation (nkcc2 alpha, nkcc2 beta, aqp1dup, aqpe), stress response (hsp70, hsp90), and thyroid metabolism (thr alpha A, thr alpha B, thr beta B, diol, dio2, dio3) were differentially expressed throughout larval development, while nkcc1 alpha, nkcc2 beta, aqp3, aqp 1dup, aqpe, hsp90, thr alpha A and dio3 showed lower expression in response to the salinity reduction. Moreover, larvae were able to keep energy metabolism related gene expression (atp6, cox1) at stable levels, irrespective of the salinity reduction. As such, when reducing salinity, an energy surplus associated to reduced osmoregulation demands and stress (lower nkcc, aqp and hsp expression), likely facilitated the observed increased survival, improved biometry and enhanced growth efficiency. Additionally, the salinity reduction decreased the amount of severe deformities such as spinal curvature and emaciation but also induced an edematous state of the larval heart, resulting in the most balanced mortality/deformity ratio when salinity was decreased on 3 dph and at 2 psu/day. However, the persistency of the pericardial edema and if or how it represents an obstacle in further larval development needs to be further clarified. In conclusion, this study clearly showed that salinity reduction regimes towards iso-osmotic conditions facilitated the European eel pre-leptocephalus development and revealed the existence of highly sensitive and regulated ... |
author2 |
DTU Aqua, National Institute of Aquatic Resources Danmarks Tekniske Universitet = Technical University of Denmark (DTU) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Helmholtz Centre for Ocean Research Kiel (GEOMAR) |
format |
Article in Journal/Newspaper |
author |
Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna J. Tomkiewicz, Jonna Butts, Ian A. E. |
author_facet |
Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna J. Tomkiewicz, Jonna Butts, Ian A. E. |
author_sort |
Politis, Sebastian N. |
title |
Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level |
title_short |
Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level |
title_full |
Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level |
title_fullStr |
Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level |
title_full_unstemmed |
Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level |
title_sort |
salinity reduction benefits european eel larvae: insights at the morphological and molecular level |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal.science/hal-02640324 https://hal.science/hal-02640324/document https://hal.science/hal-02640324/file/Politis_etal_POne_2018.pdf https://doi.org/10.1371/journal.pone.0198294 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_source |
ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE https://hal.science/hal-02640324 PLoS ONE, 2018, 13 (6), pp.e0198294. ⟨10.1371/journal.pone.0198294⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0198294 hal-02640324 https://hal.science/hal-02640324 https://hal.science/hal-02640324/document https://hal.science/hal-02640324/file/Politis_etal_POne_2018.pdf doi:10.1371/journal.pone.0198294 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0198294 |
container_title |
PLOS ONE |
container_volume |
13 |
container_issue |
6 |
container_start_page |
e0198294 |
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spelling |
ftunivbrest:oai:HAL:hal-02640324v1 2024-02-11T09:55:36+01:00 Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna J. Tomkiewicz, Jonna Butts, Ian A. E. DTU Aqua, National Institute of Aquatic Resources Danmarks Tekniske Universitet = Technical University of Denmark (DTU) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Helmholtz Centre for Ocean Research Kiel (GEOMAR) 2018 https://hal.science/hal-02640324 https://hal.science/hal-02640324/document https://hal.science/hal-02640324/file/Politis_etal_POne_2018.pdf https://doi.org/10.1371/journal.pone.0198294 en eng HAL CCSD Public Library of Science info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0198294 hal-02640324 https://hal.science/hal-02640324 https://hal.science/hal-02640324/document https://hal.science/hal-02640324/file/Politis_etal_POne_2018.pdf doi:10.1371/journal.pone.0198294 info:eu-repo/semantics/OpenAccess ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE https://hal.science/hal-02640324 PLoS ONE, 2018, 13 (6), pp.e0198294. ⟨10.1371/journal.pone.0198294⟩ ACL embryonic-development japanese eel early-life stages gene-expression water temperature anguilla-japonica artificial maturation cultured larvae thyroid-hormone receptors yolk resorption [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BA.ZV]Life Sciences [q-bio]/Animal biology/Vertebrate Zoology [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis info:eu-repo/semantics/article Journal articles 2018 ftunivbrest https://doi.org/10.1371/journal.pone.0198294 2024-01-23T23:39:56Z WOS:000435090700050 International audience European eel (Anguilla anguilla) is a euryhaline species, that has adapted to cope with both, hyper- and hypo-osmotic environments. This study investigates the effect of salinity, from a morphological and molecular point of view on European eel larvae reared from 0 to 12 days post hatch (dph). Offspring reared in 36 practical salinity units (psu; control), were compared with larvae reared in six scenarios, where salinity was decreased on 0 or 3 dph and in rates of 1, 2 or 4 psu/day, towards iso-osmotic conditions. Results showed that several genes relating to osmoregulation (nkcc2 alpha, nkcc2 beta, aqp1dup, aqpe), stress response (hsp70, hsp90), and thyroid metabolism (thr alpha A, thr alpha B, thr beta B, diol, dio2, dio3) were differentially expressed throughout larval development, while nkcc1 alpha, nkcc2 beta, aqp3, aqp 1dup, aqpe, hsp90, thr alpha A and dio3 showed lower expression in response to the salinity reduction. Moreover, larvae were able to keep energy metabolism related gene expression (atp6, cox1) at stable levels, irrespective of the salinity reduction. As such, when reducing salinity, an energy surplus associated to reduced osmoregulation demands and stress (lower nkcc, aqp and hsp expression), likely facilitated the observed increased survival, improved biometry and enhanced growth efficiency. Additionally, the salinity reduction decreased the amount of severe deformities such as spinal curvature and emaciation but also induced an edematous state of the larval heart, resulting in the most balanced mortality/deformity ratio when salinity was decreased on 3 dph and at 2 psu/day. However, the persistency of the pericardial edema and if or how it represents an obstacle in further larval development needs to be further clarified. In conclusion, this study clearly showed that salinity reduction regimes towards iso-osmotic conditions facilitated the European eel pre-leptocephalus development and revealed the existence of highly sensitive and regulated ... Article in Journal/Newspaper Anguilla anguilla European eel Université de Bretagne Occidentale: HAL PLOS ONE 13 6 e0198294 |