Profundibacter Amoris Gen. Nov., Sp. Nov., a New Member of the Roseobacter Clade Isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge
International audience A bacterial strain, designated BAR1T, was isolated from a microbial mat growing on the surface of a barite chimney at the Loki’s Castle Vent Field, at a depth of 2216 m. Cells of strain BAR1T were rod-shaped, Gram-reaction-negative and grew on marine broth 2216 at 10–37 °C (op...
Published in: | International Journal of Systematic and Evolutionary Microbiology |
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Online Access: | https://hal.science/hal-02373589 https://doi.org/10.1099/ijsem.0.003234 |
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ftunivbrest:oai:HAL:hal-02373589v1 2024-02-11T10:01:29+01:00 Profundibacter Amoris Gen. Nov., Sp. Nov., a New Member of the Roseobacter Clade Isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge Bauer, Sven Le Moine Sjøberg, Andreas Gilje L'Haridon, Stéphane Stokke, Runar Roalkvam, Irene Steen, Ida Helene Dahle, Håkon University of Bergen (UiB) Laboratoire de Microbiologie des Environnements Extrêmophiles (LM2E) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS) Institute of Theoretical Astrophysics Oslo University of Oslo (UiO) 2019 https://hal.science/hal-02373589 https://doi.org/10.1099/ijsem.0.003234 en eng HAL CCSD Microbiology Society info:eu-repo/semantics/altIdentifier/doi/10.1099/ijsem.0.003234 info:eu-repo/semantics/altIdentifier/pmid/30758283 hal-02373589 https://hal.science/hal-02373589 doi:10.1099/ijsem.0.003234 PUBMED: 30758283 http://creativecommons.org/licenses/by/ ISSN: 1466-5026 EISSN: 1466-5034 International Journal of Systematic and Evolutionary Microbiology https://hal.science/hal-02373589 International Journal of Systematic and Evolutionary Microbiology, 2019, 69 (4), pp.975-981. ⟨10.1099/ijsem.0.003234⟩ roseobacter alphaproteobacteria piezophilic isolate deep-sea hydrothermal vent [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology info:eu-repo/semantics/article Journal articles 2019 ftunivbrest https://doi.org/10.1099/ijsem.0.003234 2024-01-23T23:40:21Z International audience A bacterial strain, designated BAR1T, was isolated from a microbial mat growing on the surface of a barite chimney at the Loki’s Castle Vent Field, at a depth of 2216 m. Cells of strain BAR1T were rod-shaped, Gram-reaction-negative and grew on marine broth 2216 at 10–37 °C (optimum 27–35 °C), pH 5.5–8.0 (optimum pH 6.5–7.5) and 0.5–5.0 % NaCl (optimum 2 %). The DNA G+C content was 57.38 mol%. The membrane-associated major ubiquinone was Q-10, the fatty acid profile was dominated by C18 : 1ω7c (91 %), and the polar lipids detected were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid, one unidentified lipid and one unidentified phospholipid. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain BAR1T clustered together with Rhodobacterales bacterium PRT1, as well as the genera Halocynthiibacter and Pseudohalocynthiibacter in a polyphyletic clade within the Roseobacter clade. Several characteristics differentiate strain BAR1T from the aforementioned genera, including its motility, its piezophilic behaviour and its ability to grow at 35 °C and under anaerobic conditions. Accordingly, strain BAR1T is considered to represent a novel genus and species within the Roseobacter clade, for which the name Profundibacter amoris gen. nov., sp. nov. is proposed. The type strain is Profundibacter amoris BAR1T (=JCM 31874T=DSM 104147T). Article in Journal/Newspaper Arctic Université de Bretagne Occidentale: HAL Arctic International Journal of Systematic and Evolutionary Microbiology 69 4 975 981 |
institution |
Open Polar |
collection |
Université de Bretagne Occidentale: HAL |
op_collection_id |
ftunivbrest |
language |
English |
topic |
roseobacter alphaproteobacteria piezophilic isolate deep-sea hydrothermal vent [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
spellingShingle |
roseobacter alphaproteobacteria piezophilic isolate deep-sea hydrothermal vent [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Bauer, Sven Le Moine Sjøberg, Andreas Gilje L'Haridon, Stéphane Stokke, Runar Roalkvam, Irene Steen, Ida Helene Dahle, Håkon Profundibacter Amoris Gen. Nov., Sp. Nov., a New Member of the Roseobacter Clade Isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge |
topic_facet |
roseobacter alphaproteobacteria piezophilic isolate deep-sea hydrothermal vent [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
description |
International audience A bacterial strain, designated BAR1T, was isolated from a microbial mat growing on the surface of a barite chimney at the Loki’s Castle Vent Field, at a depth of 2216 m. Cells of strain BAR1T were rod-shaped, Gram-reaction-negative and grew on marine broth 2216 at 10–37 °C (optimum 27–35 °C), pH 5.5–8.0 (optimum pH 6.5–7.5) and 0.5–5.0 % NaCl (optimum 2 %). The DNA G+C content was 57.38 mol%. The membrane-associated major ubiquinone was Q-10, the fatty acid profile was dominated by C18 : 1ω7c (91 %), and the polar lipids detected were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid, one unidentified lipid and one unidentified phospholipid. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain BAR1T clustered together with Rhodobacterales bacterium PRT1, as well as the genera Halocynthiibacter and Pseudohalocynthiibacter in a polyphyletic clade within the Roseobacter clade. Several characteristics differentiate strain BAR1T from the aforementioned genera, including its motility, its piezophilic behaviour and its ability to grow at 35 °C and under anaerobic conditions. Accordingly, strain BAR1T is considered to represent a novel genus and species within the Roseobacter clade, for which the name Profundibacter amoris gen. nov., sp. nov. is proposed. The type strain is Profundibacter amoris BAR1T (=JCM 31874T=DSM 104147T). |
author2 |
University of Bergen (UiB) Laboratoire de Microbiologie des Environnements Extrêmophiles (LM2E) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS) Institute of Theoretical Astrophysics Oslo University of Oslo (UiO) |
format |
Article in Journal/Newspaper |
author |
Bauer, Sven Le Moine Sjøberg, Andreas Gilje L'Haridon, Stéphane Stokke, Runar Roalkvam, Irene Steen, Ida Helene Dahle, Håkon |
author_facet |
Bauer, Sven Le Moine Sjøberg, Andreas Gilje L'Haridon, Stéphane Stokke, Runar Roalkvam, Irene Steen, Ida Helene Dahle, Håkon |
author_sort |
Bauer, Sven Le Moine |
title |
Profundibacter Amoris Gen. Nov., Sp. Nov., a New Member of the Roseobacter Clade Isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge |
title_short |
Profundibacter Amoris Gen. Nov., Sp. Nov., a New Member of the Roseobacter Clade Isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge |
title_full |
Profundibacter Amoris Gen. Nov., Sp. Nov., a New Member of the Roseobacter Clade Isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge |
title_fullStr |
Profundibacter Amoris Gen. Nov., Sp. Nov., a New Member of the Roseobacter Clade Isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge |
title_full_unstemmed |
Profundibacter Amoris Gen. Nov., Sp. Nov., a New Member of the Roseobacter Clade Isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge |
title_sort |
profundibacter amoris gen. nov., sp. nov., a new member of the roseobacter clade isolated from loki's castle vent field on the arctic mid-ocean ridge |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://hal.science/hal-02373589 https://doi.org/10.1099/ijsem.0.003234 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
ISSN: 1466-5026 EISSN: 1466-5034 International Journal of Systematic and Evolutionary Microbiology https://hal.science/hal-02373589 International Journal of Systematic and Evolutionary Microbiology, 2019, 69 (4), pp.975-981. ⟨10.1099/ijsem.0.003234⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1099/ijsem.0.003234 info:eu-repo/semantics/altIdentifier/pmid/30758283 hal-02373589 https://hal.science/hal-02373589 doi:10.1099/ijsem.0.003234 PUBMED: 30758283 |
op_rights |
http://creativecommons.org/licenses/by/ |
op_doi |
https://doi.org/10.1099/ijsem.0.003234 |
container_title |
International Journal of Systematic and Evolutionary Microbiology |
container_volume |
69 |
container_issue |
4 |
container_start_page |
975 |
op_container_end_page |
981 |
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1790597295004188672 |