Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication
International audience Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve (Crassostrea gigas),...
Published in: | Journal of Experimental Biology |
---|---|
Main Authors: | , , , , , , , , , , , , , |
Other Authors: | , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2017
|
Subjects: | |
Online Access: | https://hal.science/hal-01632365 https://hal.science/hal-01632365/document https://hal.science/hal-01632365/file/Pauletto-2017-JExpBiol-Long.pdf https://doi.org/10.1242/jeb.156299 |
id |
ftunivbrest:oai:HAL:hal-01632365v1 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
Université de Bretagne Occidentale: HAL |
op_collection_id |
ftunivbrest |
language |
English |
topic |
Inhibitor of NF-κB RNA interference Marine bivalve Ostreid herpesvirus 1 ACL Anti-viral response [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
Inhibitor of NF-κB RNA interference Marine bivalve Ostreid herpesvirus 1 ACL Anti-viral response [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology Pauletto, Marianna Segarra, Ameíie Montagnani, Caroline Quillien, Virgile Faury, Nicole Le Grand, Jacqueline Miner, Philippe Petton, Bruno Labreuche, Yannick Fleury, Elodie Fabioux, Caroline Bargelloni, Luca Renault, Tristan Huvet, Arnaud Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication |
topic_facet |
Inhibitor of NF-κB RNA interference Marine bivalve Ostreid herpesvirus 1 ACL Anti-viral response [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve (Crassostrea gigas), as well as the specific role of the subunit 2 of the nuclear factor κB inhibitor (IκB2). This gene is a candidate of particular interest for functional investigations in the context of oyster mass mortality events, as Cg-IκB2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-IκB2 and green fluorescent protein genes were injected in vivo into oysters before being challenged by OsHV-1. Survival appeared close to 100% in both dsRNA-injected conditions associated with a low detection of viral DNA and a low expression of a panel of 39 OsHV-1 genes as compared with infected control. Long dsRNA molecules, both Cg-IκB2-and GFP-dsRNA, may have induced an anti-viral state controlling the OsHV-1 replication and precluding the understanding of the specific role of Cg-IκB2. Immune-related genes including Cg-IκB1, Cg-Rel1, Cg-IFI44, Cg-PKR and Cg-IAP appeared activated in the dsRNA-injected condition, potentially hampering viral replication and thus conferring a better resistance to OsHV-1 infection. We revealed that long dsRNA-mediated genetic interference triggered an anti-viral state in the oyster, emphasizing the need for new reverse genetics tools for assessing immune gene function and avoiding off-target effects in bivalves. |
author2 |
Università degli Studi di Padova = University of Padua (Unipd) Unité Santé, Génétique et Microbiologie des Mollusques (SGMM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff = Roscoff Marine Station (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Station biologique de Roscoff = Roscoff Marine Station (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) Unité Amélioration génétique, Santé animale et Environnement (AGSAE) ANR-08-GENM-0041,Gametogenes,Génomiques de la gamétogénèse chez l'huître creuse Crassostrea gigas(2008) European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010) |
format |
Article in Journal/Newspaper |
author |
Pauletto, Marianna Segarra, Ameíie Montagnani, Caroline Quillien, Virgile Faury, Nicole Le Grand, Jacqueline Miner, Philippe Petton, Bruno Labreuche, Yannick Fleury, Elodie Fabioux, Caroline Bargelloni, Luca Renault, Tristan Huvet, Arnaud |
author_facet |
Pauletto, Marianna Segarra, Ameíie Montagnani, Caroline Quillien, Virgile Faury, Nicole Le Grand, Jacqueline Miner, Philippe Petton, Bruno Labreuche, Yannick Fleury, Elodie Fabioux, Caroline Bargelloni, Luca Renault, Tristan Huvet, Arnaud |
author_sort |
Pauletto, Marianna |
title |
Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication |
title_short |
Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication |
title_full |
Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication |
title_fullStr |
Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication |
title_full_unstemmed |
Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication |
title_sort |
long dsrnas promote an anti-viral response in pacific oyster hampering ostreid herpesvirus 1 replication |
publisher |
HAL CCSD |
publishDate |
2017 |
url |
https://hal.science/hal-01632365 https://hal.science/hal-01632365/document https://hal.science/hal-01632365/file/Pauletto-2017-JExpBiol-Long.pdf https://doi.org/10.1242/jeb.156299 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0022-0949 EISSN: 1477-9145 Journal of Experimental Biology https://hal.science/hal-01632365 Journal of Experimental Biology, 2017, 220 (20), pp.3671 - 3685. ⟨10.1242/jeb.156299⟩ https://jeb.biologists.org/content/220/20/3671 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1242/jeb.156299 info:eu-repo/grantAgreement/EC/FP7/245119/EU/REsearch to improve PROduction of SEED of established and emerging bivalve species in European hatcheries/REPROSEED hal-01632365 https://hal.science/hal-01632365 https://hal.science/hal-01632365/document https://hal.science/hal-01632365/file/Pauletto-2017-JExpBiol-Long.pdf doi:10.1242/jeb.156299 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1242/jeb.156299 |
container_title |
Journal of Experimental Biology |
_version_ |
1798844933714477056 |
spelling |
ftunivbrest:oai:HAL:hal-01632365v1 2024-05-12T08:02:47+00:00 Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication Pauletto, Marianna Segarra, Ameíie Montagnani, Caroline Quillien, Virgile Faury, Nicole Le Grand, Jacqueline Miner, Philippe Petton, Bruno Labreuche, Yannick Fleury, Elodie Fabioux, Caroline Bargelloni, Luca Renault, Tristan Huvet, Arnaud Università degli Studi di Padova = University of Padua (Unipd) Unité Santé, Génétique et Microbiologie des Mollusques (SGMM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff = Roscoff Marine Station (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Station biologique de Roscoff = Roscoff Marine Station (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) Unité Amélioration génétique, Santé animale et Environnement (AGSAE) ANR-08-GENM-0041,Gametogenes,Génomiques de la gamétogénèse chez l'huître creuse Crassostrea gigas(2008) European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010) 2017-10-15 https://hal.science/hal-01632365 https://hal.science/hal-01632365/document https://hal.science/hal-01632365/file/Pauletto-2017-JExpBiol-Long.pdf https://doi.org/10.1242/jeb.156299 en eng HAL CCSD The Company of Biologists info:eu-repo/semantics/altIdentifier/doi/10.1242/jeb.156299 info:eu-repo/grantAgreement/EC/FP7/245119/EU/REsearch to improve PROduction of SEED of established and emerging bivalve species in European hatcheries/REPROSEED hal-01632365 https://hal.science/hal-01632365 https://hal.science/hal-01632365/document https://hal.science/hal-01632365/file/Pauletto-2017-JExpBiol-Long.pdf doi:10.1242/jeb.156299 info:eu-repo/semantics/OpenAccess ISSN: 0022-0949 EISSN: 1477-9145 Journal of Experimental Biology https://hal.science/hal-01632365 Journal of Experimental Biology, 2017, 220 (20), pp.3671 - 3685. ⟨10.1242/jeb.156299⟩ https://jeb.biologists.org/content/220/20/3671 Inhibitor of NF-κB RNA interference Marine bivalve Ostreid herpesvirus 1 ACL Anti-viral response [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2017 ftunivbrest https://doi.org/10.1242/jeb.156299 2024-04-17T23:55:25Z International audience Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve (Crassostrea gigas), as well as the specific role of the subunit 2 of the nuclear factor κB inhibitor (IκB2). This gene is a candidate of particular interest for functional investigations in the context of oyster mass mortality events, as Cg-IκB2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-IκB2 and green fluorescent protein genes were injected in vivo into oysters before being challenged by OsHV-1. Survival appeared close to 100% in both dsRNA-injected conditions associated with a low detection of viral DNA and a low expression of a panel of 39 OsHV-1 genes as compared with infected control. Long dsRNA molecules, both Cg-IκB2-and GFP-dsRNA, may have induced an anti-viral state controlling the OsHV-1 replication and precluding the understanding of the specific role of Cg-IκB2. Immune-related genes including Cg-IκB1, Cg-Rel1, Cg-IFI44, Cg-PKR and Cg-IAP appeared activated in the dsRNA-injected condition, potentially hampering viral replication and thus conferring a better resistance to OsHV-1 infection. We revealed that long dsRNA-mediated genetic interference triggered an anti-viral state in the oyster, emphasizing the need for new reverse genetics tools for assessing immune gene function and avoiding off-target effects in bivalves. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Bretagne Occidentale: HAL Pacific Journal of Experimental Biology |