Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1)
International audience Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-rel...
Published in: | Aquaculture Environment Interactions |
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Online Access: | https://hal.science/hal-01483190 https://doi.org/10.3354/aei00197 |
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ftunivbrest:oai:HAL:hal-01483190v1 2024-04-21T08:00:41+00:00 Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) Fuhrmann, Marine Petton, Bruno Quillien, Virgile Faury, Nicole Morga, Benjamin Pernet, Fabrice Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Unité Amélioration génétique, Santé animale et Environnement (AGSAE) ANR-12-AGRO-0001,GIGASSAT,Adaptation des écosystèmes ostréicoles au changement global(2012) 2016 https://hal.science/hal-01483190 https://doi.org/10.3354/aei00197 en eng HAL CCSD Inter-reseach science publisher info:eu-repo/semantics/altIdentifier/doi/10.3354/aei00197 hal-01483190 https://hal.science/hal-01483190 doi:10.3354/aei00197 ISSN: 1869-215X Aquaculture Environment Interactions https://hal.science/hal-01483190 Aquaculture Environment Interactions, 2016, 8, pp.543-552. ⟨10.3354/aei00197⟩ herpes-virus Herpesviridae haplosporidium-nelsoni msx Infection mu-var bivalve bivalve mollusks Disease transmission environments host Pacific oysters parasite pcr Risk analysis transmission ACL [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2016 ftunivbrest https://doi.org/10.3354/aei00197 2024-04-10T23:51:25Z International audience Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-related effects on the pathogen itself. To obtain donors for OsHV-1 transmission, we transferred laboratory-raised oysters to an estuary during a disease outbreak and then back to the laboratory. Oysters that tested OsHV-1 positive were placed in seawater tanks (35%, 21 degrees C). Water from these tanks was used to infect naive oysters in 2 experimental setups: (1) oysters acclimated or non-acclimated to a salinity of 10, 15, 25 and 35% and (2) oysters acclimated to a salinity of 25%; the latter were exposed to OsHV-1 water diluted to a salinity of 10 or 25%. The survival of oysters exposed to OsHV-1 water and acclimated to a salinity of 10% was \textgreater 95%, compared to only 43 to 73% survival in oysters acclimated to higher salinities (Expt 1), reflecting differences in the levels of OsHV-1 DNA and viral gene expression (Expts 1 and 2). However, the survival of their non-acclimated counterparts was only 23% (Expt 2), and the levels of OsHV-1 DNA and the expression of 4 viral genes were low (Expt 1). Thus, OsHV-1 may not have been the ultimate cause of mortality in non-acclimated oysters weakened by a salinity shock. It appears that reducing disease risk by means of low salinity is unlikely in the field. Article in Journal/Newspaper Crassostrea gigas Université de Bretagne Occidentale: HAL Aquaculture Environment Interactions 8 543 552 |
institution |
Open Polar |
collection |
Université de Bretagne Occidentale: HAL |
op_collection_id |
ftunivbrest |
language |
English |
topic |
herpes-virus Herpesviridae haplosporidium-nelsoni msx Infection mu-var bivalve bivalve mollusks Disease transmission environments host Pacific oysters parasite pcr Risk analysis transmission ACL [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
herpes-virus Herpesviridae haplosporidium-nelsoni msx Infection mu-var bivalve bivalve mollusks Disease transmission environments host Pacific oysters parasite pcr Risk analysis transmission ACL [SDE.BE]Environmental Sciences/Biodiversity and Ecology Fuhrmann, Marine Petton, Bruno Quillien, Virgile Faury, Nicole Morga, Benjamin Pernet, Fabrice Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) |
topic_facet |
herpes-virus Herpesviridae haplosporidium-nelsoni msx Infection mu-var bivalve bivalve mollusks Disease transmission environments host Pacific oysters parasite pcr Risk analysis transmission ACL [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-related effects on the pathogen itself. To obtain donors for OsHV-1 transmission, we transferred laboratory-raised oysters to an estuary during a disease outbreak and then back to the laboratory. Oysters that tested OsHV-1 positive were placed in seawater tanks (35%, 21 degrees C). Water from these tanks was used to infect naive oysters in 2 experimental setups: (1) oysters acclimated or non-acclimated to a salinity of 10, 15, 25 and 35% and (2) oysters acclimated to a salinity of 25%; the latter were exposed to OsHV-1 water diluted to a salinity of 10 or 25%. The survival of oysters exposed to OsHV-1 water and acclimated to a salinity of 10% was \textgreater 95%, compared to only 43 to 73% survival in oysters acclimated to higher salinities (Expt 1), reflecting differences in the levels of OsHV-1 DNA and viral gene expression (Expts 1 and 2). However, the survival of their non-acclimated counterparts was only 23% (Expt 2), and the levels of OsHV-1 DNA and the expression of 4 viral genes were low (Expt 1). Thus, OsHV-1 may not have been the ultimate cause of mortality in non-acclimated oysters weakened by a salinity shock. It appears that reducing disease risk by means of low salinity is unlikely in the field. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Unité Amélioration génétique, Santé animale et Environnement (AGSAE) ANR-12-AGRO-0001,GIGASSAT,Adaptation des écosystèmes ostréicoles au changement global(2012) |
format |
Article in Journal/Newspaper |
author |
Fuhrmann, Marine Petton, Bruno Quillien, Virgile Faury, Nicole Morga, Benjamin Pernet, Fabrice |
author_facet |
Fuhrmann, Marine Petton, Bruno Quillien, Virgile Faury, Nicole Morga, Benjamin Pernet, Fabrice |
author_sort |
Fuhrmann, Marine |
title |
Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) |
title_short |
Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) |
title_full |
Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) |
title_fullStr |
Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) |
title_full_unstemmed |
Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) |
title_sort |
salinity influences disease-induced mortality of the oyster crassostrea gigas and infectivity of the ostreid herpesvirus 1 (oshv-1) |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://hal.science/hal-01483190 https://doi.org/10.3354/aei00197 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 1869-215X Aquaculture Environment Interactions https://hal.science/hal-01483190 Aquaculture Environment Interactions, 2016, 8, pp.543-552. ⟨10.3354/aei00197⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3354/aei00197 hal-01483190 https://hal.science/hal-01483190 doi:10.3354/aei00197 |
op_doi |
https://doi.org/10.3354/aei00197 |
container_title |
Aquaculture Environment Interactions |
container_volume |
8 |
container_start_page |
543 |
op_container_end_page |
552 |
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1796941122728099840 |