Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts
International audience Genitors of the Pacific oyster Crassostrea gigas were submitted during gametogenesis to a short pulse exposure to the herbicide diuron at a realistic environmental concentration. Histological analysis showed no effect of diuron on gametogenesis course, sex ratio and reproducti...
Published in: | Environmental Science and Pollution Research |
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Main Authors: | , , , , , , , , , , , , , , |
Other Authors: | , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2016
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Subjects: | |
Online Access: | https://hal.science/hal-01363931 https://hal.science/hal-01363931/document https://hal.science/hal-01363931/file/Akcha-2016-EnvSciPollutRes-Effects-MANUSCRIT.pdf https://doi.org/10.1007/s11356-015-5969-2 |
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ftunivbrest:oai:HAL:hal-01363931v1 |
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openpolar |
institution |
Open Polar |
collection |
Université de Bretagne Occidentale: HAL |
op_collection_id |
ftunivbrest |
language |
English |
topic |
Reproduction Oyster Pesticide RT-qPCR Gene expression Histology Immunity Steroid ACL [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
Reproduction Oyster Pesticide RT-qPCR Gene expression Histology Immunity Steroid ACL [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology Akcha, F. Barranger, A. Bachère, Evelyne Heude Berthelin, C. Piquemal, D. Alonso, P. Rondon Sallan, R. Dimastrogiovanni, G. Porte, C. Menard, D. Szczybelski, A. Benabdelmouna, A Auffret, Michel Rouxel, J. Burgeot, T. Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts |
topic_facet |
Reproduction Oyster Pesticide RT-qPCR Gene expression Histology Immunity Steroid ACL [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Genitors of the Pacific oyster Crassostrea gigas were submitted during gametogenesis to a short pulse exposure to the herbicide diuron at a realistic environmental concentration. Histological analysis showed no effect of diuron on gametogenesis course, sex ratio and reproductive effort. A non-significant increase in testosterone and progesterone levels was observed in genitors exposed to the herbicide. At cell level, diuron exposure was shown to modulate the phagocytic activity of circulating hemocytes. The results of a transcriptional analysis showed that diuron affected the expression of genes belonging to functions known to play a major role during oyster gametogenesis such as gene transcription regulation, DNA replication and repair, DNA methylation and cytokinesis. Taking into account the results we previously obtained on the same genitors, this study showed a negative effect of diuron on oyster reproduction by inducing both structural and functional modifications of the DNA |
author2 |
Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) ACOBIOM Institute of Environmental Assessment and Water Research (IDAEA) Consejo Superior de Investigaciones Cientificas España = Spanish National Research Council Spain (CSIC) Centro de Investigaciones Biológicas (CSIC) Unité Amélioration génétique, Santé animale et Environnement (AGSAE) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) ANR-11-CESA-0016,GIMEPEC,Génotoxicité, IMmunotoxicité et rEprotoxicité des Pesticides chez Crassostrea gigas(2011) |
format |
Article in Journal/Newspaper |
author |
Akcha, F. Barranger, A. Bachère, Evelyne Heude Berthelin, C. Piquemal, D. Alonso, P. Rondon Sallan, R. Dimastrogiovanni, G. Porte, C. Menard, D. Szczybelski, A. Benabdelmouna, A Auffret, Michel Rouxel, J. Burgeot, T. |
author_facet |
Akcha, F. Barranger, A. Bachère, Evelyne Heude Berthelin, C. Piquemal, D. Alonso, P. Rondon Sallan, R. Dimastrogiovanni, G. Porte, C. Menard, D. Szczybelski, A. Benabdelmouna, A Auffret, Michel Rouxel, J. Burgeot, T. |
author_sort |
Akcha, F. |
title |
Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts |
title_short |
Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts |
title_full |
Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts |
title_fullStr |
Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts |
title_full_unstemmed |
Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts |
title_sort |
effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://hal.science/hal-01363931 https://hal.science/hal-01363931/document https://hal.science/hal-01363931/file/Akcha-2016-EnvSciPollutRes-Effects-MANUSCRIT.pdf https://doi.org/10.1007/s11356-015-5969-2 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0944-1344 EISSN: 1614-7499 Environmental Science and Pollution Research https://hal.science/hal-01363931 Environmental Science and Pollution Research, 2016, 23 (8), pp.8008-8020. ⟨10.1007/s11356-015-5969-2⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11356-015-5969-2 hal-01363931 https://hal.science/hal-01363931 https://hal.science/hal-01363931/document https://hal.science/hal-01363931/file/Akcha-2016-EnvSciPollutRes-Effects-MANUSCRIT.pdf doi:10.1007/s11356-015-5969-2 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1007/s11356-015-5969-2 |
container_title |
Environmental Science and Pollution Research |
container_volume |
23 |
container_issue |
8 |
container_start_page |
8008 |
op_container_end_page |
8020 |
_version_ |
1797581675401576448 |
spelling |
ftunivbrest:oai:HAL:hal-01363931v1 2024-04-28T08:16:37+00:00 Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts Akcha, F. Barranger, A. Bachère, Evelyne Heude Berthelin, C. Piquemal, D. Alonso, P. Rondon Sallan, R. Dimastrogiovanni, G. Porte, C. Menard, D. Szczybelski, A. Benabdelmouna, A Auffret, Michel Rouxel, J. Burgeot, T. Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) ACOBIOM Institute of Environmental Assessment and Water Research (IDAEA) Consejo Superior de Investigaciones Cientificas España = Spanish National Research Council Spain (CSIC) Centro de Investigaciones Biológicas (CSIC) Unité Amélioration génétique, Santé animale et Environnement (AGSAE) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) ANR-11-CESA-0016,GIMEPEC,Génotoxicité, IMmunotoxicité et rEprotoxicité des Pesticides chez Crassostrea gigas(2011) 2016-04 https://hal.science/hal-01363931 https://hal.science/hal-01363931/document https://hal.science/hal-01363931/file/Akcha-2016-EnvSciPollutRes-Effects-MANUSCRIT.pdf https://doi.org/10.1007/s11356-015-5969-2 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s11356-015-5969-2 hal-01363931 https://hal.science/hal-01363931 https://hal.science/hal-01363931/document https://hal.science/hal-01363931/file/Akcha-2016-EnvSciPollutRes-Effects-MANUSCRIT.pdf doi:10.1007/s11356-015-5969-2 info:eu-repo/semantics/OpenAccess ISSN: 0944-1344 EISSN: 1614-7499 Environmental Science and Pollution Research https://hal.science/hal-01363931 Environmental Science and Pollution Research, 2016, 23 (8), pp.8008-8020. ⟨10.1007/s11356-015-5969-2⟩ Reproduction Oyster Pesticide RT-qPCR Gene expression Histology Immunity Steroid ACL [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2016 ftunivbrest https://doi.org/10.1007/s11356-015-5969-2 2024-04-17T23:53:48Z International audience Genitors of the Pacific oyster Crassostrea gigas were submitted during gametogenesis to a short pulse exposure to the herbicide diuron at a realistic environmental concentration. Histological analysis showed no effect of diuron on gametogenesis course, sex ratio and reproductive effort. A non-significant increase in testosterone and progesterone levels was observed in genitors exposed to the herbicide. At cell level, diuron exposure was shown to modulate the phagocytic activity of circulating hemocytes. The results of a transcriptional analysis showed that diuron affected the expression of genes belonging to functions known to play a major role during oyster gametogenesis such as gene transcription regulation, DNA replication and repair, DNA methylation and cytokinesis. Taking into account the results we previously obtained on the same genitors, this study showed a negative effect of diuron on oyster reproduction by inducing both structural and functional modifications of the DNA Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Bretagne Occidentale: HAL Environmental Science and Pollution Research 23 8 8008 8020 |