Effect of temperature, food availability, and estradiol injection on gametogenesis and gender in the pearl oyster Pinctada margaritifera
International audience The black-lip pearl oyster Pinctada margaritifera is a protandrous hermaphrodite species. Its economic value has led to the development of controlled hatchery reproduction techniques, although many aspects remain to be optimized. In order to understand reproductive mechanisms...
Published in: | Journal of Experimental Zoology Part A: Ecological Genetics and Physiology |
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Main Authors: | , , , , , , , , |
Other Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2016
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Subjects: | |
Online Access: | https://hal.science/hal-01260132 https://hal.science/hal-01260132/document https://hal.science/hal-01260132/file/Teaniniuraitemoana-2016-JExZool-Effect.pdf https://doi.org/10.1002/jez.1992 |
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ftunivbrest:oai:HAL:hal-01260132v1 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Université de Bretagne Occidentale: HAL |
op_collection_id |
ftunivbrest |
language |
English |
topic |
GROWTH DIFFERENTIATION STEROIDS SCALLOP TUAMOTU ARCHIPELAGO DEPENDENT SEX DETERMINATION GONADAL DEVELOPMENT PACIFIC OYSTER PATINOPECTEN-YESSOENSIS RUDITAPES-DECUSSATUS ACL [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
GROWTH DIFFERENTIATION STEROIDS SCALLOP TUAMOTU ARCHIPELAGO DEPENDENT SEX DETERMINATION GONADAL DEVELOPMENT PACIFIC OYSTER PATINOPECTEN-YESSOENSIS RUDITAPES-DECUSSATUS ACL [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology Teaniniuraitemoana, V. Leprêtre, M. Levy, P. Vanaa, V. Parrad, S. Gaertner-Mazouni, Nabila Gueguen, Yannick Huvet, Arnaud Le Moullac, Gilles Effect of temperature, food availability, and estradiol injection on gametogenesis and gender in the pearl oyster Pinctada margaritifera |
topic_facet |
GROWTH DIFFERENTIATION STEROIDS SCALLOP TUAMOTU ARCHIPELAGO DEPENDENT SEX DETERMINATION GONADAL DEVELOPMENT PACIFIC OYSTER PATINOPECTEN-YESSOENSIS RUDITAPES-DECUSSATUS ACL [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience The black-lip pearl oyster Pinctada margaritifera is a protandrous hermaphrodite species. Its economic value has led to the development of controlled hatchery reproduction techniques, although many aspects remain to be optimized. In order to understand reproductive mechanisms and their controlling factors, two independent experiments were designed to test hypotheses of gametogenesis and sex ratio control by environmental and hormonal factors. In one, pearl oysters were exposed under controlled conditions at different combinations of temperature (24 and 28°C) and food level (10,000 and 40,000 cells mL−1); whereas in the other, pearl oysters were conditioned under natural conditions into the lagoon and subjected to successive 17β-estradiol injections (100 μg per injection). Gametogenesis and sex ratio were assessed by histology for each treatment. In parallel, mRNA expressions of nine marker genes of the sexual pathway (pmarg-foxl2, pmarg-c43476, pmarg-c45042, pmarg-c19309, pmarg-c54338, pmarg-vit6, pmarg-zglp1, pmarg-dmrt, and pmarg-fem1-like) were investigated. Maximum maturation was observed in the treatment combining the highest temperature (28°C) and the highest microalgae concentration (40,000 cells mL−1), where the female sex tended to be maintained. Injection of 17β-estradiol induced a significant increase of undetermined stage proportion 2 weeks after the final injection. These results suggest that gametogenesis and gender in adult pearl oysters can be controlled by environmental factors and estrogens. While there were no significant effects on relative gene expression, the 3-gene-pair expression ratio model of the sexual pathway of P. margaritifera, suggest a probable dominance of genetic sex determinism without excluding a mixed sex determination mode (genetic + environmental). |
author2 |
Ecosystèmes Insulaires Océaniens (UMR 241) (EIO) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de la Polynésie Française (UPF)-Institut Louis Malardé Papeete (ILM) Institut de Recherche pour le Développement (IRD) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Université de la Polynésie Française (UPF) Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) ANR-11-AGRO-0006,POLYPERL,Gestion intégrée et adaptation de la perliculture en Polynésie française dans le contexte du changement global : approche environnementale, économique et sociale(2011) |
format |
Article in Journal/Newspaper |
author |
Teaniniuraitemoana, V. Leprêtre, M. Levy, P. Vanaa, V. Parrad, S. Gaertner-Mazouni, Nabila Gueguen, Yannick Huvet, Arnaud Le Moullac, Gilles |
author_facet |
Teaniniuraitemoana, V. Leprêtre, M. Levy, P. Vanaa, V. Parrad, S. Gaertner-Mazouni, Nabila Gueguen, Yannick Huvet, Arnaud Le Moullac, Gilles |
author_sort |
Teaniniuraitemoana, V. |
title |
Effect of temperature, food availability, and estradiol injection on gametogenesis and gender in the pearl oyster Pinctada margaritifera |
title_short |
Effect of temperature, food availability, and estradiol injection on gametogenesis and gender in the pearl oyster Pinctada margaritifera |
title_full |
Effect of temperature, food availability, and estradiol injection on gametogenesis and gender in the pearl oyster Pinctada margaritifera |
title_fullStr |
Effect of temperature, food availability, and estradiol injection on gametogenesis and gender in the pearl oyster Pinctada margaritifera |
title_full_unstemmed |
Effect of temperature, food availability, and estradiol injection on gametogenesis and gender in the pearl oyster Pinctada margaritifera |
title_sort |
effect of temperature, food availability, and estradiol injection on gametogenesis and gender in the pearl oyster pinctada margaritifera |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://hal.science/hal-01260132 https://hal.science/hal-01260132/document https://hal.science/hal-01260132/file/Teaniniuraitemoana-2016-JExZool-Effect.pdf https://doi.org/10.1002/jez.1992 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_source |
ISSN: 1932-5223 EISSN: 1932-5231 Journal of Experimental Zoology Part A: Ecological Genetics and Physiology https://hal.science/hal-01260132 Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, 2016, 325 (1), pp.13-24. ⟨10.1002/jez.1992⟩ http://onlinelibrary.wiley.com/doi/10.1002/jez.1992/abstract |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1002/jez.1992 hal-01260132 https://hal.science/hal-01260132 https://hal.science/hal-01260132/document https://hal.science/hal-01260132/file/Teaniniuraitemoana-2016-JExZool-Effect.pdf doi:10.1002/jez.1992 IRD: PAR00014175 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1002/jez.199210.1002/jez.1992/abstract |
container_title |
Journal of Experimental Zoology Part A: Ecological Genetics and Physiology |
container_volume |
325 |
container_issue |
1 |
container_start_page |
13 |
op_container_end_page |
24 |
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1796317427598884864 |
spelling |
ftunivbrest:oai:HAL:hal-01260132v1 2024-04-14T08:18:02+00:00 Effect of temperature, food availability, and estradiol injection on gametogenesis and gender in the pearl oyster Pinctada margaritifera Teaniniuraitemoana, V. Leprêtre, M. Levy, P. Vanaa, V. Parrad, S. Gaertner-Mazouni, Nabila Gueguen, Yannick Huvet, Arnaud Le Moullac, Gilles Ecosystèmes Insulaires Océaniens (UMR 241) (EIO) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de la Polynésie Française (UPF)-Institut Louis Malardé Papeete (ILM) Institut de Recherche pour le Développement (IRD) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Université de la Polynésie Française (UPF) Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) ANR-11-AGRO-0006,POLYPERL,Gestion intégrée et adaptation de la perliculture en Polynésie française dans le contexte du changement global : approche environnementale, économique et sociale(2011) 2016-01-01 https://hal.science/hal-01260132 https://hal.science/hal-01260132/document https://hal.science/hal-01260132/file/Teaniniuraitemoana-2016-JExZool-Effect.pdf https://doi.org/10.1002/jez.1992 en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1002/jez.1992 hal-01260132 https://hal.science/hal-01260132 https://hal.science/hal-01260132/document https://hal.science/hal-01260132/file/Teaniniuraitemoana-2016-JExZool-Effect.pdf doi:10.1002/jez.1992 IRD: PAR00014175 info:eu-repo/semantics/OpenAccess ISSN: 1932-5223 EISSN: 1932-5231 Journal of Experimental Zoology Part A: Ecological Genetics and Physiology https://hal.science/hal-01260132 Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, 2016, 325 (1), pp.13-24. ⟨10.1002/jez.1992⟩ http://onlinelibrary.wiley.com/doi/10.1002/jez.1992/abstract GROWTH DIFFERENTIATION STEROIDS SCALLOP TUAMOTU ARCHIPELAGO DEPENDENT SEX DETERMINATION GONADAL DEVELOPMENT PACIFIC OYSTER PATINOPECTEN-YESSOENSIS RUDITAPES-DECUSSATUS ACL [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2016 ftunivbrest https://doi.org/10.1002/jez.199210.1002/jez.1992/abstract 2024-03-21T16:25:52Z International audience The black-lip pearl oyster Pinctada margaritifera is a protandrous hermaphrodite species. Its economic value has led to the development of controlled hatchery reproduction techniques, although many aspects remain to be optimized. In order to understand reproductive mechanisms and their controlling factors, two independent experiments were designed to test hypotheses of gametogenesis and sex ratio control by environmental and hormonal factors. In one, pearl oysters were exposed under controlled conditions at different combinations of temperature (24 and 28°C) and food level (10,000 and 40,000 cells mL−1); whereas in the other, pearl oysters were conditioned under natural conditions into the lagoon and subjected to successive 17β-estradiol injections (100 μg per injection). Gametogenesis and sex ratio were assessed by histology for each treatment. In parallel, mRNA expressions of nine marker genes of the sexual pathway (pmarg-foxl2, pmarg-c43476, pmarg-c45042, pmarg-c19309, pmarg-c54338, pmarg-vit6, pmarg-zglp1, pmarg-dmrt, and pmarg-fem1-like) were investigated. Maximum maturation was observed in the treatment combining the highest temperature (28°C) and the highest microalgae concentration (40,000 cells mL−1), where the female sex tended to be maintained. Injection of 17β-estradiol induced a significant increase of undetermined stage proportion 2 weeks after the final injection. These results suggest that gametogenesis and gender in adult pearl oysters can be controlled by environmental factors and estrogens. While there were no significant effects on relative gene expression, the 3-gene-pair expression ratio model of the sexual pathway of P. margaritifera, suggest a probable dominance of genetic sex determinism without excluding a mixed sex determination mode (genetic + environmental). Article in Journal/Newspaper Pacific oyster Université de Bretagne Occidentale: HAL Pacific Journal of Experimental Zoology Part A: Ecological Genetics and Physiology 325 1 13 24 |