Mitochondrial activity, hemocyte parameters and lipid composition modulation by dietary conditioning in the Pacific oyster Crassostrea gigas.
International audience Several parameters can affect membrane lipid composition in bivalves, including diet. Although two fatty acids (FA) 22:6n-3 and 20:5n-3 are essential membrane components, they are sparingly synthesized by bivalves and must be obtained from their diet. Here, effects of dietary...
Published in: | Journal of Comparative Physiology B |
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Main Authors: | , , , , , |
Other Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2014
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Subjects: | |
Online Access: | https://hal.univ-brest.fr/hal-00948647 https://doi.org/10.1007/s00360-013-0800-1 |
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ftunivbrest:oai:HAL:hal-00948647v1 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Université de Bretagne Occidentale: HAL |
op_collection_id |
ftunivbrest |
language |
English |
topic |
ACL Crassostrea gigas Diet Fatty acid Hemocytes Mitochondria Phospholipids [SDE]Environmental Sciences [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
ACL Crassostrea gigas Diet Fatty acid Hemocytes Mitochondria Phospholipids [SDE]Environmental Sciences [SDE.BE]Environmental Sciences/Biodiversity and Ecology Dudognon, Tony Lambert, Christophe Quere, Claudie Auffret, Michel Soudant, Philippe Kraffe, Edouard Mitochondrial activity, hemocyte parameters and lipid composition modulation by dietary conditioning in the Pacific oyster Crassostrea gigas. |
topic_facet |
ACL Crassostrea gigas Diet Fatty acid Hemocytes Mitochondria Phospholipids [SDE]Environmental Sciences [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Several parameters can affect membrane lipid composition in bivalves, including diet. Although two fatty acids (FA) 22:6n-3 and 20:5n-3 are essential membrane components, they are sparingly synthesized by bivalves and must be obtained from their diet. Here, effects of dietary modifications of membrane lipid composition were studied at both cellular and subcellular levels in the oyster Crassostrea gigas. To this end, we compared oysters fed two monoalgal diets that differed markedly in their FA composition and a mix of both. As expected, algae impacted phospholipids, in particular 22:6n-3 and 20:5n-3, reflecting differences of dietary microalgae FA composition. Meantime, total saturated FA, total monounsaturated FA, total polyunsaturated FA and total non-methylene-interrupted FA varied little and phospholipid class composition was only slightly affected by diets. Measures made in hemocytes indicated that only mitochondrial membrane potential was affected by diets. Total ROS production as well as mitochondrial superoxide production did not differ with diet. There was no difference in phosphorylating (state 3) and non-phosphorylating (state 4) rates of oxygen consumption rates or in cytochrome c oxidase activity of mitochondria isolated from gills between the three diets. Similarly, neither cytochromes a, b, c or c 1 content nor citrate synthase activities were changed, suggesting that number and morphology of mitochondria were not affected by dietary treatment. These results suggest that oysters could possess high homeostatic capabilities, at both cellular and subcellular levels, to minimize the effect of dietary FA and related membrane lipid FA modifications on mitochondrial functions. These capabilities could be a means to face variations in diet composition in their natural environment and to preserve important oyster physiological functions such as growth and reproduction. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Physiologie des Invertébrés (LPI) Physiologie Fonctionnelle des Organismes Marins (PFOM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
format |
Article in Journal/Newspaper |
author |
Dudognon, Tony Lambert, Christophe Quere, Claudie Auffret, Michel Soudant, Philippe Kraffe, Edouard |
author_facet |
Dudognon, Tony Lambert, Christophe Quere, Claudie Auffret, Michel Soudant, Philippe Kraffe, Edouard |
author_sort |
Dudognon, Tony |
title |
Mitochondrial activity, hemocyte parameters and lipid composition modulation by dietary conditioning in the Pacific oyster Crassostrea gigas. |
title_short |
Mitochondrial activity, hemocyte parameters and lipid composition modulation by dietary conditioning in the Pacific oyster Crassostrea gigas. |
title_full |
Mitochondrial activity, hemocyte parameters and lipid composition modulation by dietary conditioning in the Pacific oyster Crassostrea gigas. |
title_fullStr |
Mitochondrial activity, hemocyte parameters and lipid composition modulation by dietary conditioning in the Pacific oyster Crassostrea gigas. |
title_full_unstemmed |
Mitochondrial activity, hemocyte parameters and lipid composition modulation by dietary conditioning in the Pacific oyster Crassostrea gigas. |
title_sort |
mitochondrial activity, hemocyte parameters and lipid composition modulation by dietary conditioning in the pacific oyster crassostrea gigas. |
publisher |
HAL CCSD |
publishDate |
2014 |
url |
https://hal.univ-brest.fr/hal-00948647 https://doi.org/10.1007/s00360-013-0800-1 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0174-1578 EISSN: 1432-136X Journal of Comparative Physiology B https://hal.univ-brest.fr/hal-00948647 Journal of Comparative Physiology B, 2014, 184 (3), pp.303-317. ⟨10.1007/s00360-013-0800-1⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00360-013-0800-1 info:eu-repo/semantics/altIdentifier/pmid/24441864 hal-00948647 https://hal.univ-brest.fr/hal-00948647 doi:10.1007/s00360-013-0800-1 PUBMED: 24441864 |
op_doi |
https://doi.org/10.1007/s00360-013-0800-1 |
container_title |
Journal of Comparative Physiology B |
container_volume |
184 |
container_issue |
3 |
container_start_page |
303 |
op_container_end_page |
317 |
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1790599220472840192 |
spelling |
ftunivbrest:oai:HAL:hal-00948647v1 2024-02-11T10:03:04+01:00 Mitochondrial activity, hemocyte parameters and lipid composition modulation by dietary conditioning in the Pacific oyster Crassostrea gigas. Dudognon, Tony Lambert, Christophe Quere, Claudie Auffret, Michel Soudant, Philippe Kraffe, Edouard Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Physiologie des Invertébrés (LPI) Physiologie Fonctionnelle des Organismes Marins (PFOM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) 2014-01-18 https://hal.univ-brest.fr/hal-00948647 https://doi.org/10.1007/s00360-013-0800-1 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s00360-013-0800-1 info:eu-repo/semantics/altIdentifier/pmid/24441864 hal-00948647 https://hal.univ-brest.fr/hal-00948647 doi:10.1007/s00360-013-0800-1 PUBMED: 24441864 ISSN: 0174-1578 EISSN: 1432-136X Journal of Comparative Physiology B https://hal.univ-brest.fr/hal-00948647 Journal of Comparative Physiology B, 2014, 184 (3), pp.303-317. ⟨10.1007/s00360-013-0800-1⟩ ACL Crassostrea gigas Diet Fatty acid Hemocytes Mitochondria Phospholipids [SDE]Environmental Sciences [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2014 ftunivbrest https://doi.org/10.1007/s00360-013-0800-1 2024-01-23T23:35:56Z International audience Several parameters can affect membrane lipid composition in bivalves, including diet. Although two fatty acids (FA) 22:6n-3 and 20:5n-3 are essential membrane components, they are sparingly synthesized by bivalves and must be obtained from their diet. Here, effects of dietary modifications of membrane lipid composition were studied at both cellular and subcellular levels in the oyster Crassostrea gigas. To this end, we compared oysters fed two monoalgal diets that differed markedly in their FA composition and a mix of both. As expected, algae impacted phospholipids, in particular 22:6n-3 and 20:5n-3, reflecting differences of dietary microalgae FA composition. Meantime, total saturated FA, total monounsaturated FA, total polyunsaturated FA and total non-methylene-interrupted FA varied little and phospholipid class composition was only slightly affected by diets. Measures made in hemocytes indicated that only mitochondrial membrane potential was affected by diets. Total ROS production as well as mitochondrial superoxide production did not differ with diet. There was no difference in phosphorylating (state 3) and non-phosphorylating (state 4) rates of oxygen consumption rates or in cytochrome c oxidase activity of mitochondria isolated from gills between the three diets. Similarly, neither cytochromes a, b, c or c 1 content nor citrate synthase activities were changed, suggesting that number and morphology of mitochondria were not affected by dietary treatment. These results suggest that oysters could possess high homeostatic capabilities, at both cellular and subcellular levels, to minimize the effect of dietary FA and related membrane lipid FA modifications on mitochondrial functions. These capabilities could be a means to face variations in diet composition in their natural environment and to preserve important oyster physiological functions such as growth and reproduction. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Bretagne Occidentale: HAL Pacific Journal of Comparative Physiology B 184 3 303 317 |