Activation of the MAPKs ERK1/2 by cell swelling in turbot hepatocytes.
International audience BACKGROUND INFORMATION: Activation of MAPKs (mitogen-activated protein kinases), in particular ERK1/2 (extracellular-signal-regulated kinase 1/2), has been reported to take place in a large variety of cell types after hypo-osmotic cell swelling. Depending on cell type, ERK1/2...
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Online Access: | https://hal.univ-brest.fr/hal-00837573 https://doi.org/10.1042/BC20090154 |
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ftunivbrest:oai:HAL:hal-00837573v1 2024-01-07T09:47:09+01:00 Activation of the MAPKs ERK1/2 by cell swelling in turbot hepatocytes. Fouchs, Audrey Ollivier, Hélène Haond, Christophe Roy, Stella Calvès, Patrick Pichavant-Rafini, Karine Optimisation des régulations physiologiques (ORPHY (EA 4324)) Université de Brest (UBO)-Centre Hospitalier Régional Universitaire de Brest (CHRU Brest)-Institut Brestois Santé Agro Matière (IBSAM) Université de Brest (UBO)-Université de Brest (UBO) 2010-08 https://hal.univ-brest.fr/hal-00837573 https://doi.org/10.1042/BC20090154 en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1042/BC20090154 info:eu-repo/semantics/altIdentifier/pmid/20377528 hal-00837573 https://hal.univ-brest.fr/hal-00837573 doi:10.1042/BC20090154 PUBMED: 20377528 ISSN: 0248-4900 EISSN: 1768-322X Biology of the Cell https://hal.univ-brest.fr/hal-00837573 Biology of the Cell, 2010, 102 (8), pp.447-56. ⟨10.1042/BC20090154⟩ MESH: Adenosine Triphosphate MESH: Animals MESH: Enzyme Activation MESH: Flatfishes MESH: Hepatocytes MESH: Hypotonic Solutions MESH: Mitogen-Activated Protein Kinase 1 MESH: Mitogen-Activated Protein Kinase 3 MESH: Osmotic Pressure MESH: Phosphorylation MESH: Receptors Purinergic P2 MESH: Thapsigargin MESH: Calcium MESH: Cell Size MESH: Egtazic Acid [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] info:eu-repo/semantics/article Journal articles 2010 ftunivbrest https://doi.org/10.1042/BC20090154 2023-12-12T23:35:57Z International audience BACKGROUND INFORMATION: Activation of MAPKs (mitogen-activated protein kinases), in particular ERK1/2 (extracellular-signal-regulated kinase 1/2), has been reported to take place in a large variety of cell types after hypo-osmotic cell swelling. Depending on cell type, ERK1/2 phosphorylation can then serve or not the RVD (regulatory volume decrease) process. The present study investigates ERK1/2 activation after aniso-osmotic stimulations in turbot hepatocytes and the potential link between phosphorylation of these proteins and RVD. RESULTS: In turbot hepatocytes, Western-blot analysis shows that a hypo-osmotic shock from 320 to 240 mOsm kg(-1) induced a rapid increase in ERK1/2 phosphorylation, whereas a hyper-osmotic shock from 320 to 400 mOsm kg(-1) induced no significant change in the phosphorylation of these proteins. The hypo-osmotic-induced ERK1/2 phosphorylation was significantly prevented when hypo-osmotic shock was performed in the presence of the specific MEK (MAPK/ERK kinase) inhibitor PD98059 (100 microM). In these conditions, the RVD process was not altered, suggesting that ERK1/2 did not participate in this process in turbot hepatocytes. Moreover, the hypo-osmotic-induced activation of ERK1/2 was significantly prevented by breakdown of extracellular ATP by apyrase (10 units ml(-1)), by inhibition of purinergic P2 receptors by suramin (100 microM) or by calcium depletion using EGTA (1 mM) and thapsigargin (1 microM). CONCLUSIONS: In turbot hepatocytes, hypo-osmotic swelling but not hyper-osmotic shrinkage induced the activation of ERK1/2. However, these proteins do not seem to be involved in the RVD process. Their hypo-osmotic-induced activation is partially due to cascades of signalling events triggered by the binding of released ATP on purinergic P2 receptors and requires the presence of calcium. Article in Journal/Newspaper Turbot Université de Bretagne Occidentale: HAL Mek ENVELOPE(7.086,7.086,62.735,62.735) Biology of the Cell 102 8 447 456 |
institution |
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Université de Bretagne Occidentale: HAL |
op_collection_id |
ftunivbrest |
language |
English |
topic |
MESH: Adenosine Triphosphate MESH: Animals MESH: Enzyme Activation MESH: Flatfishes MESH: Hepatocytes MESH: Hypotonic Solutions MESH: Mitogen-Activated Protein Kinase 1 MESH: Mitogen-Activated Protein Kinase 3 MESH: Osmotic Pressure MESH: Phosphorylation MESH: Receptors Purinergic P2 MESH: Thapsigargin MESH: Calcium MESH: Cell Size MESH: Egtazic Acid [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] |
spellingShingle |
MESH: Adenosine Triphosphate MESH: Animals MESH: Enzyme Activation MESH: Flatfishes MESH: Hepatocytes MESH: Hypotonic Solutions MESH: Mitogen-Activated Protein Kinase 1 MESH: Mitogen-Activated Protein Kinase 3 MESH: Osmotic Pressure MESH: Phosphorylation MESH: Receptors Purinergic P2 MESH: Thapsigargin MESH: Calcium MESH: Cell Size MESH: Egtazic Acid [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] Fouchs, Audrey Ollivier, Hélène Haond, Christophe Roy, Stella Calvès, Patrick Pichavant-Rafini, Karine Activation of the MAPKs ERK1/2 by cell swelling in turbot hepatocytes. |
topic_facet |
MESH: Adenosine Triphosphate MESH: Animals MESH: Enzyme Activation MESH: Flatfishes MESH: Hepatocytes MESH: Hypotonic Solutions MESH: Mitogen-Activated Protein Kinase 1 MESH: Mitogen-Activated Protein Kinase 3 MESH: Osmotic Pressure MESH: Phosphorylation MESH: Receptors Purinergic P2 MESH: Thapsigargin MESH: Calcium MESH: Cell Size MESH: Egtazic Acid [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] |
description |
International audience BACKGROUND INFORMATION: Activation of MAPKs (mitogen-activated protein kinases), in particular ERK1/2 (extracellular-signal-regulated kinase 1/2), has been reported to take place in a large variety of cell types after hypo-osmotic cell swelling. Depending on cell type, ERK1/2 phosphorylation can then serve or not the RVD (regulatory volume decrease) process. The present study investigates ERK1/2 activation after aniso-osmotic stimulations in turbot hepatocytes and the potential link between phosphorylation of these proteins and RVD. RESULTS: In turbot hepatocytes, Western-blot analysis shows that a hypo-osmotic shock from 320 to 240 mOsm kg(-1) induced a rapid increase in ERK1/2 phosphorylation, whereas a hyper-osmotic shock from 320 to 400 mOsm kg(-1) induced no significant change in the phosphorylation of these proteins. The hypo-osmotic-induced ERK1/2 phosphorylation was significantly prevented when hypo-osmotic shock was performed in the presence of the specific MEK (MAPK/ERK kinase) inhibitor PD98059 (100 microM). In these conditions, the RVD process was not altered, suggesting that ERK1/2 did not participate in this process in turbot hepatocytes. Moreover, the hypo-osmotic-induced activation of ERK1/2 was significantly prevented by breakdown of extracellular ATP by apyrase (10 units ml(-1)), by inhibition of purinergic P2 receptors by suramin (100 microM) or by calcium depletion using EGTA (1 mM) and thapsigargin (1 microM). CONCLUSIONS: In turbot hepatocytes, hypo-osmotic swelling but not hyper-osmotic shrinkage induced the activation of ERK1/2. However, these proteins do not seem to be involved in the RVD process. Their hypo-osmotic-induced activation is partially due to cascades of signalling events triggered by the binding of released ATP on purinergic P2 receptors and requires the presence of calcium. |
author2 |
Optimisation des régulations physiologiques (ORPHY (EA 4324)) Université de Brest (UBO)-Centre Hospitalier Régional Universitaire de Brest (CHRU Brest)-Institut Brestois Santé Agro Matière (IBSAM) Université de Brest (UBO)-Université de Brest (UBO) |
format |
Article in Journal/Newspaper |
author |
Fouchs, Audrey Ollivier, Hélène Haond, Christophe Roy, Stella Calvès, Patrick Pichavant-Rafini, Karine |
author_facet |
Fouchs, Audrey Ollivier, Hélène Haond, Christophe Roy, Stella Calvès, Patrick Pichavant-Rafini, Karine |
author_sort |
Fouchs, Audrey |
title |
Activation of the MAPKs ERK1/2 by cell swelling in turbot hepatocytes. |
title_short |
Activation of the MAPKs ERK1/2 by cell swelling in turbot hepatocytes. |
title_full |
Activation of the MAPKs ERK1/2 by cell swelling in turbot hepatocytes. |
title_fullStr |
Activation of the MAPKs ERK1/2 by cell swelling in turbot hepatocytes. |
title_full_unstemmed |
Activation of the MAPKs ERK1/2 by cell swelling in turbot hepatocytes. |
title_sort |
activation of the mapks erk1/2 by cell swelling in turbot hepatocytes. |
publisher |
HAL CCSD |
publishDate |
2010 |
url |
https://hal.univ-brest.fr/hal-00837573 https://doi.org/10.1042/BC20090154 |
long_lat |
ENVELOPE(7.086,7.086,62.735,62.735) |
geographic |
Mek |
geographic_facet |
Mek |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
ISSN: 0248-4900 EISSN: 1768-322X Biology of the Cell https://hal.univ-brest.fr/hal-00837573 Biology of the Cell, 2010, 102 (8), pp.447-56. ⟨10.1042/BC20090154⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1042/BC20090154 info:eu-repo/semantics/altIdentifier/pmid/20377528 hal-00837573 https://hal.univ-brest.fr/hal-00837573 doi:10.1042/BC20090154 PUBMED: 20377528 |
op_doi |
https://doi.org/10.1042/BC20090154 |
container_title |
Biology of the Cell |
container_volume |
102 |
container_issue |
8 |
container_start_page |
447 |
op_container_end_page |
456 |
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1787429121729495040 |