Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas.
International audience Gonadotropin-releasing hormone (GnRH), a key neuropeptide regulating reproduction in vertebrates has now been characterized in a number of non-vertebrate species. Despite the demonstration of its ancestral origin, the structure and the function of this family of peptides remai...
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Format: | Article in Journal/Newspaper |
Language: | English |
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Online Access: | https://hal.univ-brest.fr/hal-00788233 https://doi.org/10.1016/j.peptides.2012.01.017 |
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ftunivbrest:oai:HAL:hal-00788233v1 2024-02-11T10:03:06+01:00 Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas. Bigot, Laetitia Zatylny-Gaudin, Céline Rodet, Franck Bernay, Benoit Boudry, Pierre Favrel, Pascal Biologie des mollusques marins et des écosystèmes associés (BioMEA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Spectrométrie de Masse Biologique Fondamentale et Appliquée Université de Lille, Sciences et Technologies Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2012-04 https://hal.univ-brest.fr/hal-00788233 https://doi.org/10.1016/j.peptides.2012.01.017 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.peptides.2012.01.017 info:eu-repo/semantics/altIdentifier/pmid/22306476 hal-00788233 https://hal.univ-brest.fr/hal-00788233 doi:10.1016/j.peptides.2012.01.017 PUBMED: 22306476 ISSN: 0196-9781 EISSN: 1873-5169 Peptides https://hal.univ-brest.fr/hal-00788233 Peptides, 2012, 34 (2), pp.303-10. ⟨10.1016/j.peptides.2012.01.017⟩ Mollusk Bivalve Oyster Neuroendocrinology Reproduction GnRH [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2012 ftunivbrest https://doi.org/10.1016/j.peptides.2012.01.017 2024-01-23T23:36:17Z International audience Gonadotropin-releasing hormone (GnRH), a key neuropeptide regulating reproduction in vertebrates has now been characterized in a number of non-vertebrate species. Despite the demonstration of its ancestral origin, the structure and the function of this family of peptides remain poorly known in species as distant as lophotrochozoans. In this study, two GnRH-related peptides (Cg-GnRH-a and CgGnRH-G) were characterized by mass spectrometry from extracts of the visceral ganglia of the Pacific oyster Crassostrea gigas. These peptides showed a high degree of sequence identity with GnRHs of other mollusks and annelids and to a lesser extent with those of vertebrates or with AKH and corazonins of insects. Both the mature peptides and the transcript encoding the precursor protein were exclusively expressed in the visceral ganglia. Significant differences in transcriptional activity of Cg-GnRH encoding gene were recorded in the ganglia along the reproductive cycle and according to trophic conditions with a higher level in fed animals compared to starved animals. This suggests the involvement of Cg-GnRHs as synchronizers of nutritional status with energy requirements during reproduction in oyster. Evidence for a role of Cg-GnRHs as neuroregulators and as neuroendocrine factors in bivalve is discussed. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Bretagne Occidentale: HAL Pacific Peptides 34 2 303 310 |
institution |
Open Polar |
collection |
Université de Bretagne Occidentale: HAL |
op_collection_id |
ftunivbrest |
language |
English |
topic |
Mollusk Bivalve Oyster Neuroendocrinology Reproduction GnRH [SDE]Environmental Sciences |
spellingShingle |
Mollusk Bivalve Oyster Neuroendocrinology Reproduction GnRH [SDE]Environmental Sciences Bigot, Laetitia Zatylny-Gaudin, Céline Rodet, Franck Bernay, Benoit Boudry, Pierre Favrel, Pascal Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas. |
topic_facet |
Mollusk Bivalve Oyster Neuroendocrinology Reproduction GnRH [SDE]Environmental Sciences |
description |
International audience Gonadotropin-releasing hormone (GnRH), a key neuropeptide regulating reproduction in vertebrates has now been characterized in a number of non-vertebrate species. Despite the demonstration of its ancestral origin, the structure and the function of this family of peptides remain poorly known in species as distant as lophotrochozoans. In this study, two GnRH-related peptides (Cg-GnRH-a and CgGnRH-G) were characterized by mass spectrometry from extracts of the visceral ganglia of the Pacific oyster Crassostrea gigas. These peptides showed a high degree of sequence identity with GnRHs of other mollusks and annelids and to a lesser extent with those of vertebrates or with AKH and corazonins of insects. Both the mature peptides and the transcript encoding the precursor protein were exclusively expressed in the visceral ganglia. Significant differences in transcriptional activity of Cg-GnRH encoding gene were recorded in the ganglia along the reproductive cycle and according to trophic conditions with a higher level in fed animals compared to starved animals. This suggests the involvement of Cg-GnRHs as synchronizers of nutritional status with energy requirements during reproduction in oyster. Evidence for a role of Cg-GnRHs as neuroregulators and as neuroendocrine factors in bivalve is discussed. |
author2 |
Biologie des mollusques marins et des écosystèmes associés (BioMEA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Spectrométrie de Masse Biologique Fondamentale et Appliquée Université de Lille, Sciences et Technologies Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Bigot, Laetitia Zatylny-Gaudin, Céline Rodet, Franck Bernay, Benoit Boudry, Pierre Favrel, Pascal |
author_facet |
Bigot, Laetitia Zatylny-Gaudin, Céline Rodet, Franck Bernay, Benoit Boudry, Pierre Favrel, Pascal |
author_sort |
Bigot, Laetitia |
title |
Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas. |
title_short |
Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas. |
title_full |
Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas. |
title_fullStr |
Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas. |
title_full_unstemmed |
Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas. |
title_sort |
characterization of gnrh-related peptides from the pacific oyster crassostrea gigas. |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://hal.univ-brest.fr/hal-00788233 https://doi.org/10.1016/j.peptides.2012.01.017 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0196-9781 EISSN: 1873-5169 Peptides https://hal.univ-brest.fr/hal-00788233 Peptides, 2012, 34 (2), pp.303-10. ⟨10.1016/j.peptides.2012.01.017⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.peptides.2012.01.017 info:eu-repo/semantics/altIdentifier/pmid/22306476 hal-00788233 https://hal.univ-brest.fr/hal-00788233 doi:10.1016/j.peptides.2012.01.017 PUBMED: 22306476 |
op_doi |
https://doi.org/10.1016/j.peptides.2012.01.017 |
container_title |
Peptides |
container_volume |
34 |
container_issue |
2 |
container_start_page |
303 |
op_container_end_page |
310 |
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1790599245876690944 |