Evaluation of chromosomal damage by flow cytometry in turbot (Scophthalmus maximus L.) exposed to fuel oil.

• Published in: Biomarkers
• Main Authors: Goanvec, Christelle, Theron, Michaël, Poirier, Elisabeth, Le Floch, Stéphane, Laroche, Jean, Nonnotte, Liliane, Nonnotte, Guy
• Other Authors: Optimisation des régulations physiologiques (ORPHY (EA 4324)), Université de Brest (UBO)-Centre Hospitalier Régional Universitaire de Brest (CHRU Brest)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO)-Université de Brest (UBO), Centre de documentation de recherche et d'expérimentations sur les pollutions accidentelles des eaux (Cedre), Cedre, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2004
• Subjects: MESH: Animals; MESH: Cell Differentiation; MESH: Mutagens; MESH: Polycyclic Hydrocarbons; Aromatic; MESH: Spectrometry; Fluorescence; MESH: Time Factors; MESH: Water Pollutants; Chemical; MESH: Chromosomes; MESH: Cytochrome P-450 CYP1A1; MESH: Dose-Response Relationship; Drug; MESH: Environmental Monitoring; MESH: Environmental Pollutants; MESH: Fishes; MESH: Flow Cytometry; MESH: Fuel Oils; [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]; Scophthalmus maximus; Turbot
• Online Access: https://hal.univ-brest.fr/hal-00753922; https://doi.org/10.1080/13547500400027001

International audience Flatfishes, turbots (Scophthalmus maximus), were injected intraperitoneally with two doses of fuel oil number 2. Biliary metabolites were evaluated by fixed fluorescence to verify the efficiency of intoxication. Ethoxyresorufin-O-deethylase (EROD) activity was compared with chromosomal damage measured by flow cytometry. The analysis of biliary metabolites showed a good dose-response relation and constitutes a clear reference for the subsequent measurements. Comparing flow cytometry and EROD results, a shorter delay of response for EROD activity was obtained, but chromosomal damage was significant only after one week. The persistence of the EROD response was shorter, while the genotoxic signal still persisted after one month. The measurement of chromosomal damage allowed a good differentiation between the two tested doses. In the case of EROD activity, the results were less clear. The results suggest that within a few weeks after exposure to fuel oil number 2, the measurements of chromosomal damage by flow cytometry can be used to detect a dose-dependent genotoxic response in fish.