31P nuclear magnetic resonance measurements of phosphate metabolites and intracellular pH in turbot Psetta maxima red blood cells using a novel flow method.

• Published in: Journal of Fish Biology
• Main Authors: Thebault, M. T., Pichavant, K., Kervarec, N.
• Other Authors: Optimisation des régulations physiologiques (ORPHY (EA 4324)), Université de Brest (UBO)-Centre Hospitalier Régional Universitaire de Brest (CHRU Brest)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO)-Université de Brest (UBO), Service Commun RMN-RPE, Université de Brest (UBO)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2009
• Subjects: MESH: Animals; MESH: Cell Respiration; MESH: Erythrocytes; MESH: Flatfishes; MESH: Hydrogen-Ion Concentration; MESH: Intracellular Space; MESH: Magnetic Resonance Spectroscopy; MESH: Monitoring; Physiologic; MESH: Phosphates; MESH: Phosphorus Isotopes; [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]; Turbot
• Online Access: https://hal.univ-brest.fr/hal-00750436; https://doi.org/10.1111/j.1095-8649.2009.02351.x

International audience A method for oxygenating and mixing suspensions of turbot Psetta maxima red blood cells (RBC) was tested in (31)P nuclear magnetic resonance (NMR) spectroscopy. In normoxia, the levels of inorganic phosphate (Pi) and nucleoside triphosphates (NTP) were stable up to 140 min and intracellular pH (pHi) was maintained and decreased oxygen partial pressure (P(O ( 2) )) from 30 to 15 and 600 Pa produced a significant fall in the intensity of NTP resonance, balanced by an increase in the Pi signal. Treatment of RBC with 0. 5 M isoproterenol during hypoxia exposure did not affect the pattern of changes in NTP or pHi induced by hypoxia and the effect was manifest only on Pi levels.