Molecular and cellular response to short-term oxygen variations in the Pacific oyster Crassostrea gigas
International audience Intertidal organisms have to face daily high oxygen variations from hypoxic to hyperoxic conditions. To cope with such constraints, these organisms must have well developed compensatory mechanisms involving antioxidant and energetic adjustments. The oyster Crassostrea gigas is...
Published in: | Journal of Experimental Marine Biology and Ecology |
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Online Access: | https://hal.univ-brest.fr/hal-00673797 https://doi.org/10.1016/j.jembe.2011.11.007 |
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ftunivbrest:oai:HAL:hal-00673797v1 2024-06-23T07:52:17+00:00 Molecular and cellular response to short-term oxygen variations in the Pacific oyster Crassostrea gigas Sussarellu, Rossana Fabioux, Caroline Sanchez, Miguel, C. Le Goïc, Nelly Lambert, Christophe Soudant, Philippe Moraga, Dario Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2012 https://hal.univ-brest.fr/hal-00673797 https://doi.org/10.1016/j.jembe.2011.11.007 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jembe.2011.11.007 hal-00673797 https://hal.univ-brest.fr/hal-00673797 doi:10.1016/j.jembe.2011.11.007 ISSN: 0022-0981 Journal of Experimental Marine Biology and Ecology https://hal.univ-brest.fr/hal-00673797 Journal of Experimental Marine Biology and Ecology, 2012, 412 (31), pp.87-95. ⟨10.1016/j.jembe.2011.11.007⟩ Gene expression Hemocytes Hypoxia Oxidative stress Oyster Re-oxygenation [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2012 ftunivbrest https://doi.org/10.1016/j.jembe.2011.11.007 2024-06-03T23:58:26Z International audience Intertidal organisms have to face daily high oxygen variations from hypoxic to hyperoxic conditions. To cope with such constraints, these organisms must have well developed compensatory mechanisms involving antioxidant and energetic adjustments. The oyster Crassostrea gigas is regularly exposed to high oxygen fluctuations and is a good model to understand these physiological regulations. This study aimed to explore the mechanisms involved in response to short-term oxygen variations in the digestive gland and hemocytes of C. gigas. The effects of returning into water after an emersion period were tested for 24 h at two different oxygen concentrations (normoxic 8.8 mg O2 L−1 and hypoxic 2.6 mg O2 L−1). Gene expression on several antioxidant enzymes was performed. In addition, a marker of oxidative damage, the malondialdehyde (MDA) was quantified. Hemocyte parameters measured were viability, concentration of different cellular sub-populations and oxidative activity. Changes in energetic metabolism were investigated measuring mRNA levels of pyruvate kinase (PK) and phosphoenolpyruvate carboxykinase (PEPCK). Moreover, mRNA levels of the transcription factor HIFα were analyzed. Higher levels of the alternative oxidase (AOX) mRNA in normoxic conditions corroborate the hypothesis of the AOX having a role in the redox balance. MDA levels did not show any condition effect, suggesting that hypoxic conditions did not cause oxidative stress. mRNA levels of antioxidant enzymes and hemocyte oxidative activity increased during the 24 h of immersion. This suggests that the disruption of tidal cycles by maintaining oysters immersed resulted in an increase of oxygen-dependent activities. No clear hypoxic responses were detected neither in a switch of PEPCK and PK toward anaerobic metabolism, nor in HIFα regulation. PK mRNA levels were significantly higher in nomoxic conditions, suggesting that more elevated oxygen concentrations would lead to higher energetic metabolism. Hemocyte concentrations were higher ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Bretagne Occidentale: HAL Pacific Journal of Experimental Marine Biology and Ecology 412 87 95 |
institution |
Open Polar |
collection |
Université de Bretagne Occidentale: HAL |
op_collection_id |
ftunivbrest |
language |
English |
topic |
Gene expression Hemocytes Hypoxia Oxidative stress Oyster Re-oxygenation [SDE]Environmental Sciences |
spellingShingle |
Gene expression Hemocytes Hypoxia Oxidative stress Oyster Re-oxygenation [SDE]Environmental Sciences Sussarellu, Rossana Fabioux, Caroline Sanchez, Miguel, C. Le Goïc, Nelly Lambert, Christophe Soudant, Philippe Moraga, Dario Molecular and cellular response to short-term oxygen variations in the Pacific oyster Crassostrea gigas |
topic_facet |
Gene expression Hemocytes Hypoxia Oxidative stress Oyster Re-oxygenation [SDE]Environmental Sciences |
description |
International audience Intertidal organisms have to face daily high oxygen variations from hypoxic to hyperoxic conditions. To cope with such constraints, these organisms must have well developed compensatory mechanisms involving antioxidant and energetic adjustments. The oyster Crassostrea gigas is regularly exposed to high oxygen fluctuations and is a good model to understand these physiological regulations. This study aimed to explore the mechanisms involved in response to short-term oxygen variations in the digestive gland and hemocytes of C. gigas. The effects of returning into water after an emersion period were tested for 24 h at two different oxygen concentrations (normoxic 8.8 mg O2 L−1 and hypoxic 2.6 mg O2 L−1). Gene expression on several antioxidant enzymes was performed. In addition, a marker of oxidative damage, the malondialdehyde (MDA) was quantified. Hemocyte parameters measured were viability, concentration of different cellular sub-populations and oxidative activity. Changes in energetic metabolism were investigated measuring mRNA levels of pyruvate kinase (PK) and phosphoenolpyruvate carboxykinase (PEPCK). Moreover, mRNA levels of the transcription factor HIFα were analyzed. Higher levels of the alternative oxidase (AOX) mRNA in normoxic conditions corroborate the hypothesis of the AOX having a role in the redox balance. MDA levels did not show any condition effect, suggesting that hypoxic conditions did not cause oxidative stress. mRNA levels of antioxidant enzymes and hemocyte oxidative activity increased during the 24 h of immersion. This suggests that the disruption of tidal cycles by maintaining oysters immersed resulted in an increase of oxygen-dependent activities. No clear hypoxic responses were detected neither in a switch of PEPCK and PK toward anaerobic metabolism, nor in HIFα regulation. PK mRNA levels were significantly higher in nomoxic conditions, suggesting that more elevated oxygen concentrations would lead to higher energetic metabolism. Hemocyte concentrations were higher ... |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Sussarellu, Rossana Fabioux, Caroline Sanchez, Miguel, C. Le Goïc, Nelly Lambert, Christophe Soudant, Philippe Moraga, Dario |
author_facet |
Sussarellu, Rossana Fabioux, Caroline Sanchez, Miguel, C. Le Goïc, Nelly Lambert, Christophe Soudant, Philippe Moraga, Dario |
author_sort |
Sussarellu, Rossana |
title |
Molecular and cellular response to short-term oxygen variations in the Pacific oyster Crassostrea gigas |
title_short |
Molecular and cellular response to short-term oxygen variations in the Pacific oyster Crassostrea gigas |
title_full |
Molecular and cellular response to short-term oxygen variations in the Pacific oyster Crassostrea gigas |
title_fullStr |
Molecular and cellular response to short-term oxygen variations in the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Molecular and cellular response to short-term oxygen variations in the Pacific oyster Crassostrea gigas |
title_sort |
molecular and cellular response to short-term oxygen variations in the pacific oyster crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://hal.univ-brest.fr/hal-00673797 https://doi.org/10.1016/j.jembe.2011.11.007 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0022-0981 Journal of Experimental Marine Biology and Ecology https://hal.univ-brest.fr/hal-00673797 Journal of Experimental Marine Biology and Ecology, 2012, 412 (31), pp.87-95. ⟨10.1016/j.jembe.2011.11.007⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jembe.2011.11.007 hal-00673797 https://hal.univ-brest.fr/hal-00673797 doi:10.1016/j.jembe.2011.11.007 |
op_doi |
https://doi.org/10.1016/j.jembe.2011.11.007 |
container_title |
Journal of Experimental Marine Biology and Ecology |
container_volume |
412 |
container_start_page |
87 |
op_container_end_page |
95 |
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1802643539272663040 |