Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32
International audience The strategies used by bacterial pathogens to circumvent host defense mechanisms remain largely undefined in bivalve molluscs. In this study, we investigated experimentally the interactions between the Pacific oyster (Crassostrea gigas) immune system and Vibrio aestuarianus st...
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Online Access: | https://hal.univ-brest.fr/hal-00670385 https://doi.org/10.1016/j.micinf.2006.07.020 |
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ftunivbrest:oai:HAL:hal-00670385v1 2024-05-12T08:02:39+00:00 Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32 Labreuche, Yannick Lambert, Christophe Soudant, Philippe Boulo, Viviane Huvet, Arnaud Nicolas, Jean-Louis Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Unité Lagons, Ecosystèmes et Aquaculture Durable en Nouvelle-Calédonie (LEADNC) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Génome, populations, interactions, adaptation (GPIA) Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) 2006 https://hal.univ-brest.fr/hal-00670385 https://doi.org/10.1016/j.micinf.2006.07.020 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micinf.2006.07.020 hal-00670385 https://hal.univ-brest.fr/hal-00670385 doi:10.1016/j.micinf.2006.07.020 ISSN: 1286-4579 Microbes and Infection https://hal.univ-brest.fr/hal-00670385 Microbes and Infection, 2006, 8 (12-13), pp.2715-2724. ⟨10.1016/j.micinf.2006.07.020⟩ bivalve immunity Vibrio aestuarianus pathogenesis oyster Crassostrea gigas [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2006 ftunivbrest https://doi.org/10.1016/j.micinf.2006.07.020 2024-04-17T23:51:00Z International audience The strategies used by bacterial pathogens to circumvent host defense mechanisms remain largely undefined in bivalve molluscs. In this study, we investigated experimentally the interactions between the Pacific oyster (Crassostrea gigas) immune system and Vibrio aestuarianus strain 01/32, a pathogenic bacterium originally isolated from moribund oysters. First, an antibiotic-resistant V. aestuarianus strain was used to demonstrate that only a limited number of bacterial cells was detected in the host circulatory system, suggesting that the bacteria may localize in some organs. Second, we examined the host defense responses to V. aestuarianus at the cellular and molecular levels, using flow-cytometry and real-time PCR techniques. We showed that hemocyte phagocytosis and adhesive capabilities were affected during the course of infection. Our results also uncovered a previously-undescribed mechanism used by a Vibrio in the initial stages of host interaction: deregulation of the hemocyte oxidative metabolism by enhancing the production of reactive oxygen species and down-regulating superoxide dismutase (Cg-EcSOD) gene expression. This deregulation may provide an opportunity to the pathogen by impairing hemocyte functions and survival. These findings provide new insights into the cellular and molecular bases of the host-pathogen interactions in C. gigas oyster. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Bretagne Occidentale: HAL Pacific Microbes and Infection 8 12-13 2715 2724 |
institution |
Open Polar |
collection |
Université de Bretagne Occidentale: HAL |
op_collection_id |
ftunivbrest |
language |
English |
topic |
bivalve immunity Vibrio aestuarianus pathogenesis oyster Crassostrea gigas [SDE]Environmental Sciences |
spellingShingle |
bivalve immunity Vibrio aestuarianus pathogenesis oyster Crassostrea gigas [SDE]Environmental Sciences Labreuche, Yannick Lambert, Christophe Soudant, Philippe Boulo, Viviane Huvet, Arnaud Nicolas, Jean-Louis Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32 |
topic_facet |
bivalve immunity Vibrio aestuarianus pathogenesis oyster Crassostrea gigas [SDE]Environmental Sciences |
description |
International audience The strategies used by bacterial pathogens to circumvent host defense mechanisms remain largely undefined in bivalve molluscs. In this study, we investigated experimentally the interactions between the Pacific oyster (Crassostrea gigas) immune system and Vibrio aestuarianus strain 01/32, a pathogenic bacterium originally isolated from moribund oysters. First, an antibiotic-resistant V. aestuarianus strain was used to demonstrate that only a limited number of bacterial cells was detected in the host circulatory system, suggesting that the bacteria may localize in some organs. Second, we examined the host defense responses to V. aestuarianus at the cellular and molecular levels, using flow-cytometry and real-time PCR techniques. We showed that hemocyte phagocytosis and adhesive capabilities were affected during the course of infection. Our results also uncovered a previously-undescribed mechanism used by a Vibrio in the initial stages of host interaction: deregulation of the hemocyte oxidative metabolism by enhancing the production of reactive oxygen species and down-regulating superoxide dismutase (Cg-EcSOD) gene expression. This deregulation may provide an opportunity to the pathogen by impairing hemocyte functions and survival. These findings provide new insights into the cellular and molecular bases of the host-pathogen interactions in C. gigas oyster. |
author2 |
Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Unité Lagons, Ecosystèmes et Aquaculture Durable en Nouvelle-Calédonie (LEADNC) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Génome, populations, interactions, adaptation (GPIA) Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Labreuche, Yannick Lambert, Christophe Soudant, Philippe Boulo, Viviane Huvet, Arnaud Nicolas, Jean-Louis |
author_facet |
Labreuche, Yannick Lambert, Christophe Soudant, Philippe Boulo, Viviane Huvet, Arnaud Nicolas, Jean-Louis |
author_sort |
Labreuche, Yannick |
title |
Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32 |
title_short |
Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32 |
title_full |
Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32 |
title_fullStr |
Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32 |
title_full_unstemmed |
Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32 |
title_sort |
cellular and molecular hemocyte responses of the pacific oyster, crassostrea gigas, following bacterial infection with vibrio aestuarianus strain 01/32 |
publisher |
HAL CCSD |
publishDate |
2006 |
url |
https://hal.univ-brest.fr/hal-00670385 https://doi.org/10.1016/j.micinf.2006.07.020 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1286-4579 Microbes and Infection https://hal.univ-brest.fr/hal-00670385 Microbes and Infection, 2006, 8 (12-13), pp.2715-2724. ⟨10.1016/j.micinf.2006.07.020⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micinf.2006.07.020 hal-00670385 https://hal.univ-brest.fr/hal-00670385 doi:10.1016/j.micinf.2006.07.020 |
op_doi |
https://doi.org/10.1016/j.micinf.2006.07.020 |
container_title |
Microbes and Infection |
container_volume |
8 |
container_issue |
12-13 |
container_start_page |
2715 |
op_container_end_page |
2724 |
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1798844791323099136 |