Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia
International audience Marine intertidal organisms commonly face hypoxic stress during low tide emersion; moreover, eutrophic conditions and sediment nearness could lead to hypoxic phenomena; it is indeed important to understand the molecular processes involved in the response to hypoxia. In this st...
Published in: | Marine Genomics |
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Online Access: | https://hal.univ-brest.fr/hal-00645853 https://doi.org/10.1016/j.margen.2010.08.005 |
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ftunivbrest:oai:HAL:hal-00645853v1 2024-04-28T08:16:28+00:00 Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia Sussarellu, Rossana Fabioux, Caroline Le Moullac, Gilles Fleury, Elodie Moraga, Dario Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Centre Océanologique du Pacifique (COP) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) 2010 https://hal.univ-brest.fr/hal-00645853 https://doi.org/10.1016/j.margen.2010.08.005 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.margen.2010.08.005 hal-00645853 https://hal.univ-brest.fr/hal-00645853 doi:10.1016/j.margen.2010.08.005 ISSN: 1874-7787 Marine Genomics https://hal.univ-brest.fr/hal-00645853 Marine Genomics, 2010, 3 (3-4), pp.133-143. ⟨10.1016/j.margen.2010.08.005⟩ Mollusca Hypoxia cDNA microarray Gene expression Oxidative stress Respiratory chain [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2010 ftunivbrest https://doi.org/10.1016/j.margen.2010.08.005 2024-04-10T23:44:46Z International audience Marine intertidal organisms commonly face hypoxic stress during low tide emersion; moreover, eutrophic conditions and sediment nearness could lead to hypoxic phenomena; it is indeed important to understand the molecular processes involved in the response to hypoxia. In this study the molecular response of the Pacific oyster Crassostrea gigas to prolonged hypoxia (2 mg O2 L− 1 for 20 d) was investigated under experimental conditions. A transcriptomic approach was employed using a cDNA microarray of 9058 C. gigas clones to highlight the genetic expression patterns of the Pacific oyster under hypoxic conditions. Lines of oysters resistant (R) and susceptible (S) to summer mortality were used in this study. ANOVA analysis was used to identify the genes involved in the response to hypoxia in comparison to normoxic conditions. The hypoxic response was maximal at day 20. The principal biological processes up-regulated by hypoxic stress were antioxidant defense and the respiratory chain compartment, suggesting oxidative stress caused by hypoxia or an anticipatory response for normoxic recovery. This is the first study employing microarrays to characterize the genetic markers and metabolic pathways responding to hypoxic stress in C. gigas. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Bretagne Occidentale: HAL Marine Genomics 3 3-4 133 143 |
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Open Polar |
collection |
Université de Bretagne Occidentale: HAL |
op_collection_id |
ftunivbrest |
language |
English |
topic |
Mollusca Hypoxia cDNA microarray Gene expression Oxidative stress Respiratory chain [SDE]Environmental Sciences |
spellingShingle |
Mollusca Hypoxia cDNA microarray Gene expression Oxidative stress Respiratory chain [SDE]Environmental Sciences Sussarellu, Rossana Fabioux, Caroline Le Moullac, Gilles Fleury, Elodie Moraga, Dario Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia |
topic_facet |
Mollusca Hypoxia cDNA microarray Gene expression Oxidative stress Respiratory chain [SDE]Environmental Sciences |
description |
International audience Marine intertidal organisms commonly face hypoxic stress during low tide emersion; moreover, eutrophic conditions and sediment nearness could lead to hypoxic phenomena; it is indeed important to understand the molecular processes involved in the response to hypoxia. In this study the molecular response of the Pacific oyster Crassostrea gigas to prolonged hypoxia (2 mg O2 L− 1 for 20 d) was investigated under experimental conditions. A transcriptomic approach was employed using a cDNA microarray of 9058 C. gigas clones to highlight the genetic expression patterns of the Pacific oyster under hypoxic conditions. Lines of oysters resistant (R) and susceptible (S) to summer mortality were used in this study. ANOVA analysis was used to identify the genes involved in the response to hypoxia in comparison to normoxic conditions. The hypoxic response was maximal at day 20. The principal biological processes up-regulated by hypoxic stress were antioxidant defense and the respiratory chain compartment, suggesting oxidative stress caused by hypoxia or an anticipatory response for normoxic recovery. This is the first study employing microarrays to characterize the genetic markers and metabolic pathways responding to hypoxic stress in C. gigas. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Centre Océanologique du Pacifique (COP) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Sussarellu, Rossana Fabioux, Caroline Le Moullac, Gilles Fleury, Elodie Moraga, Dario |
author_facet |
Sussarellu, Rossana Fabioux, Caroline Le Moullac, Gilles Fleury, Elodie Moraga, Dario |
author_sort |
Sussarellu, Rossana |
title |
Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia |
title_short |
Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia |
title_full |
Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia |
title_fullStr |
Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia |
title_full_unstemmed |
Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia |
title_sort |
transcriptomic response of the pacific oyster crassostrea gigas to hypoxia |
publisher |
HAL CCSD |
publishDate |
2010 |
url |
https://hal.univ-brest.fr/hal-00645853 https://doi.org/10.1016/j.margen.2010.08.005 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1874-7787 Marine Genomics https://hal.univ-brest.fr/hal-00645853 Marine Genomics, 2010, 3 (3-4), pp.133-143. ⟨10.1016/j.margen.2010.08.005⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.margen.2010.08.005 hal-00645853 https://hal.univ-brest.fr/hal-00645853 doi:10.1016/j.margen.2010.08.005 |
op_doi |
https://doi.org/10.1016/j.margen.2010.08.005 |
container_title |
Marine Genomics |
container_volume |
3 |
container_issue |
3-4 |
container_start_page |
133 |
op_container_end_page |
143 |
_version_ |
1797581586158321664 |