Periphyton diversity in two different Antarctic lakes assessed using metabarcoding

Antarctic lakes have generally simple periphyton communities when compared with those of lower latitudes. To date, assessment of microbial diversity in Antarctica has relied heavily on traditional direct observation and cultivation methods. In this study, sterilized cotton baits were left submerged...

Full description

Bibliographic Details
Published in:Antarctic Science
Main Authors: Câmara, Paulo Eduardo Aguiar Saraiva, Souza, Láuren M. D. de, Pinto, Otávio Henrique Bezerra, Convey, Peter, Amorim, Eduardo T., Silva, Micheline Carvalho, Rosa, Luiz Henrique
Format: Article in Journal/Newspaper
Language:English
Published: Springer 2021
Subjects:
Antártica
Sequenciamento de alto rendimento
Espaçador ITS
Antarctic
King George Island
South Shetland Islands
Deception Island
Hennequin
Antarc*
Antarctic Science
Antarctica
Online Access:https://repositorio.unb.br/handle/10482/43590
https://doi.org/10.1017/S0954102021000316
Description
Summary:Antarctic lakes have generally simple periphyton communities when compared with those of lower latitudes. To date, assessment of microbial diversity in Antarctica has relied heavily on traditional direct observation and cultivation methods. In this study, sterilized cotton baits were left submerged for two years in two lakes on King George Island and Deception Island, South Shetland Islands (Maritime Antarctic), followed by assessment of diversity by metabarcoding using high-throughput sequencing. DNA sequences of 44 taxa belonging to four kingdoms and seven phyla were found. Thirty-six taxa were detected in Hennequin Lake on King George Island and 20 taxa were detected in Soto Lake on Deception Island. However, no significant difference in species composition was detected between the two assemblages (Shannon index). Our data suggest that metabarcoding provides a suitable method for the assessment of periphyton biodiversity in oligotrophic Antarctic lakes.

Similar Items