Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland
Twenty-eight sponge specimens were collected at a shallow water hydrothermal vent site north of Iceland. Extracts were prepared and tested in vitro for cytotoxic activity, and eight of them were shown to be cytotoxic. A mass spectrometry (MS)-based metabolomics approach was used to determine the che...
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Online Access: | http://hdl.handle.net/10863/6189 https://doi.org/10.3390/md15020052 |
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ftunivbozen:oai:bia.unibz.it:10863/6189 2023-05-15T16:47:49+02:00 Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland Einarsdottir E Magnusdottir M Astarita G Köck M Ögmundsdottir HM Thorsteinsdottir M Rapp HT Omarsdottir S Paglia G 2017 application/pdf http://hdl.handle.net/10863/6189 https://doi.org/10.3390/md15020052 en EN-GB eng 1660-3397 http://dx.doi.org/10.3390/md15020052 http://hdl.handle.net/10863/6189 Article 2017 ftunivbozen https://doi.org/10.3390/md15020052 2019-10-31T08:31:27Z Twenty-eight sponge specimens were collected at a shallow water hydrothermal vent site north of Iceland. Extracts were prepared and tested in vitro for cytotoxic activity, and eight of them were shown to be cytotoxic. A mass spectrometry (MS)-based metabolomics approach was used to determine the chemical composition of the extracts. This analysis highlighted clear differences in the metabolomes of three sponge specimens, and all of them were identified as Haliclona (Rhizoniera) rosea (Bowerbank, 1866). Therefore, these specimens were selected for further investigation. Haliclona rosea metabolomes contained a class of potential key compounds, the 3-alkyl pyridine alkaloids (3-APA) responsible for the cytotoxic activity of the fractions. Several 3-APA compounds were tentatively identified including haliclamines, cyclostellettamines, viscosalines and viscosamines. Among these compounds, cyclostellettamine P was tentatively identified for the first time by using ion mobility MS in time-aligned parallel (TAP) fragmentation mode. In this work, we show the potential of applying metabolomics strategies and in particular the utility of coupling ion mobility with MS for the molecular characterization of sponge specimens. open Article in Journal/Newspaper Iceland Free University of Bozen-Bolzano (UNIBZ): BIA (Bozen-Bolzano Institutional Archive) Marine Drugs 15 2 52 |
institution |
Open Polar |
collection |
Free University of Bozen-Bolzano (UNIBZ): BIA (Bozen-Bolzano Institutional Archive) |
op_collection_id |
ftunivbozen |
language |
English |
description |
Twenty-eight sponge specimens were collected at a shallow water hydrothermal vent site north of Iceland. Extracts were prepared and tested in vitro for cytotoxic activity, and eight of them were shown to be cytotoxic. A mass spectrometry (MS)-based metabolomics approach was used to determine the chemical composition of the extracts. This analysis highlighted clear differences in the metabolomes of three sponge specimens, and all of them were identified as Haliclona (Rhizoniera) rosea (Bowerbank, 1866). Therefore, these specimens were selected for further investigation. Haliclona rosea metabolomes contained a class of potential key compounds, the 3-alkyl pyridine alkaloids (3-APA) responsible for the cytotoxic activity of the fractions. Several 3-APA compounds were tentatively identified including haliclamines, cyclostellettamines, viscosalines and viscosamines. Among these compounds, cyclostellettamine P was tentatively identified for the first time by using ion mobility MS in time-aligned parallel (TAP) fragmentation mode. In this work, we show the potential of applying metabolomics strategies and in particular the utility of coupling ion mobility with MS for the molecular characterization of sponge specimens. open |
format |
Article in Journal/Newspaper |
author |
Einarsdottir E Magnusdottir M Astarita G Köck M Ögmundsdottir HM Thorsteinsdottir M Rapp HT Omarsdottir S Paglia G |
spellingShingle |
Einarsdottir E Magnusdottir M Astarita G Köck M Ögmundsdottir HM Thorsteinsdottir M Rapp HT Omarsdottir S Paglia G Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
author_facet |
Einarsdottir E Magnusdottir M Astarita G Köck M Ögmundsdottir HM Thorsteinsdottir M Rapp HT Omarsdottir S Paglia G |
author_sort |
Einarsdottir E |
title |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_short |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_full |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_fullStr |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_full_unstemmed |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_sort |
metabolic profiling as a screening tool for cytotoxic compounds: identification of 3-alkyl pyridine alkaloids from sponges collected at a shallow water hydrothermal vent site north of iceland |
publishDate |
2017 |
url |
http://hdl.handle.net/10863/6189 https://doi.org/10.3390/md15020052 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
1660-3397 http://dx.doi.org/10.3390/md15020052 http://hdl.handle.net/10863/6189 |
op_doi |
https://doi.org/10.3390/md15020052 |
container_title |
Marine Drugs |
container_volume |
15 |
container_issue |
2 |
container_start_page |
52 |
_version_ |
1766037928954298368 |