Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil
International audience The active layer of soil overlaying permafrost in the Arctic is subjected to annual changes in temperature and soil chemistry, which we hypothesize to affect the overall soil microbial community. We investigated changes in soil microorganisms at different temperatures during w...
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Online Access: | https://hal.inrae.fr/hal-02620200 https://doi.org/10.1038/s41396-019-0351-x |
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ftunivbourgogne:oai:HAL:hal-02620200v1 2024-02-11T09:59:37+01:00 Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil Schostag, Morten Prieme, Anders Jacquiod, Samuel Russel, Jakob Ekelund, Flemming Jacobsen, Carsten Suhr University of Copenhagen = Københavns Universitet (UCPH) Department of Biology Northern Arizona University Flagstaff Geological Survey of Denmark and Greenland (GEUS) Agroécologie Dijon Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté COMUE (UBFC) Aarhus University Aarhus Danish National Research Foundation CENPERM DNRF100 Danish Geocenter; Danish Council for Independent Research DFF-4002-00274 European Project: 675546,H2020,H2020-MSCA-ITN-2015,MicroArctic(2016) 2019 https://hal.inrae.fr/hal-02620200 https://doi.org/10.1038/s41396-019-0351-x en eng HAL CCSD Nature Publishing Group info:eu-repo/semantics/altIdentifier/doi/10.1038/s41396-019-0351-x info:eu-repo/semantics/altIdentifier/pmid/30692629 info:eu-repo/grantAgreement//675546/EU/Microorganisms in Warming Arctic Environments/MicroArctic hal-02620200 https://hal.inrae.fr/hal-02620200 doi:10.1038/s41396-019-0351-x PRODINRA: 479257 PUBMED: 30692629 WOS: 000464960400018 ISSN: 1751-7362 EISSN: 1751-7370 ISME Journal https://hal.inrae.fr/hal-02620200 ISME Journal, 2019, 13 (5), pp.1345-1359. ⟨10.1038/s41396-019-0351-x⟩ [SDV]Life Sciences [q-bio] [SDE]Environmental Sciences [SDV.BV]Life Sciences [q-bio]/Vegetal Biology info:eu-repo/semantics/article Journal articles 2019 ftunivbourgogne https://doi.org/10.1038/s41396-019-0351-x 2024-01-16T23:39:23Z International audience The active layer of soil overlaying permafrost in the Arctic is subjected to annual changes in temperature and soil chemistry, which we hypothesize to affect the overall soil microbial community. We investigated changes in soil microorganisms at different temperatures during warming and freezing of the active layer soil from Svalbard, Norway. Soil community data were obtained by direct shotgun sequencing of total extracted RNA. No changes in soil microbial communities were detected when warming from -10 to -2 degrees C or when freezing from -2 to -10 degrees C. In contrast, within a few days we observed changes when warming from -2 to +2 degrees C with a decrease in fungal rRNA and an increase in several OTUs belonging to Gemmatimonadetes, Bacteroidetes and Betaproteobacteria. Even more substantial changes occurred when incubating at 2 degrees C for 16 days, with declines in total fungal potential activity and decreases in oligotrophic members from Actinobacteria and Acidobacteria. Additionally, we detected an increase in transcriptome sequences of bacterial phyla Bacteriodetes, Firmicutes, Betaproteobacteria and Gammaproteobacteria-collectively presumed to be copiotrophic. Furthermore, we detected an increase in putative bacterivorous heterotrophic flagellates, likely due to predation upon the bacterial community via grazing. Although this grazing activity may explain relatively large changes in the bacterial community composition, no changes in total 16S rRNA gene copy number were observed and the total RNA level remained stable during the incubation. Together, these results are showing the first comprehensive ecological evaluation across prokaryotic and eukaryotic microbial communities on thawing and freezing of soil by application of the Total RNA technique. Article in Journal/Newspaper Arctic Arctic permafrost Svalbard Université de Bourgogne (UB): HAL Arctic Norway Svalbard The ISME Journal 13 5 1345 1359 |
institution |
Open Polar |
collection |
Université de Bourgogne (UB): HAL |
op_collection_id |
ftunivbourgogne |
language |
English |
topic |
[SDV]Life Sciences [q-bio] [SDE]Environmental Sciences [SDV.BV]Life Sciences [q-bio]/Vegetal Biology |
spellingShingle |
[SDV]Life Sciences [q-bio] [SDE]Environmental Sciences [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Schostag, Morten Prieme, Anders Jacquiod, Samuel Russel, Jakob Ekelund, Flemming Jacobsen, Carsten Suhr Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil |
topic_facet |
[SDV]Life Sciences [q-bio] [SDE]Environmental Sciences [SDV.BV]Life Sciences [q-bio]/Vegetal Biology |
description |
International audience The active layer of soil overlaying permafrost in the Arctic is subjected to annual changes in temperature and soil chemistry, which we hypothesize to affect the overall soil microbial community. We investigated changes in soil microorganisms at different temperatures during warming and freezing of the active layer soil from Svalbard, Norway. Soil community data were obtained by direct shotgun sequencing of total extracted RNA. No changes in soil microbial communities were detected when warming from -10 to -2 degrees C or when freezing from -2 to -10 degrees C. In contrast, within a few days we observed changes when warming from -2 to +2 degrees C with a decrease in fungal rRNA and an increase in several OTUs belonging to Gemmatimonadetes, Bacteroidetes and Betaproteobacteria. Even more substantial changes occurred when incubating at 2 degrees C for 16 days, with declines in total fungal potential activity and decreases in oligotrophic members from Actinobacteria and Acidobacteria. Additionally, we detected an increase in transcriptome sequences of bacterial phyla Bacteriodetes, Firmicutes, Betaproteobacteria and Gammaproteobacteria-collectively presumed to be copiotrophic. Furthermore, we detected an increase in putative bacterivorous heterotrophic flagellates, likely due to predation upon the bacterial community via grazing. Although this grazing activity may explain relatively large changes in the bacterial community composition, no changes in total 16S rRNA gene copy number were observed and the total RNA level remained stable during the incubation. Together, these results are showing the first comprehensive ecological evaluation across prokaryotic and eukaryotic microbial communities on thawing and freezing of soil by application of the Total RNA technique. |
author2 |
University of Copenhagen = Københavns Universitet (UCPH) Department of Biology Northern Arizona University Flagstaff Geological Survey of Denmark and Greenland (GEUS) Agroécologie Dijon Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté COMUE (UBFC) Aarhus University Aarhus Danish National Research Foundation CENPERM DNRF100 Danish Geocenter; Danish Council for Independent Research DFF-4002-00274 European Project: 675546,H2020,H2020-MSCA-ITN-2015,MicroArctic(2016) |
format |
Article in Journal/Newspaper |
author |
Schostag, Morten Prieme, Anders Jacquiod, Samuel Russel, Jakob Ekelund, Flemming Jacobsen, Carsten Suhr |
author_facet |
Schostag, Morten Prieme, Anders Jacquiod, Samuel Russel, Jakob Ekelund, Flemming Jacobsen, Carsten Suhr |
author_sort |
Schostag, Morten |
title |
Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil |
title_short |
Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil |
title_full |
Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil |
title_fullStr |
Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil |
title_full_unstemmed |
Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil |
title_sort |
bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://hal.inrae.fr/hal-02620200 https://doi.org/10.1038/s41396-019-0351-x |
geographic |
Arctic Norway Svalbard |
geographic_facet |
Arctic Norway Svalbard |
genre |
Arctic Arctic permafrost Svalbard |
genre_facet |
Arctic Arctic permafrost Svalbard |
op_source |
ISSN: 1751-7362 EISSN: 1751-7370 ISME Journal https://hal.inrae.fr/hal-02620200 ISME Journal, 2019, 13 (5), pp.1345-1359. ⟨10.1038/s41396-019-0351-x⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41396-019-0351-x info:eu-repo/semantics/altIdentifier/pmid/30692629 info:eu-repo/grantAgreement//675546/EU/Microorganisms in Warming Arctic Environments/MicroArctic hal-02620200 https://hal.inrae.fr/hal-02620200 doi:10.1038/s41396-019-0351-x PRODINRA: 479257 PUBMED: 30692629 WOS: 000464960400018 |
op_doi |
https://doi.org/10.1038/s41396-019-0351-x |
container_title |
The ISME Journal |
container_volume |
13 |
container_issue |
5 |
container_start_page |
1345 |
op_container_end_page |
1359 |
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1790595434508451840 |