From Sanger to NGS: Detecting MHC (Major Histocompatibility Complex) Class II and OR (Olfactory Receptors) Genetic Variability in Italian Wolves (Canis Lupus) and relative Canids
In this PhD thesis I will describe different aspects of conservation genetics and genomics of two wild Canidae species, the wolf (Canis lupus) and the golden jackal (Canis aureus), through the study of two of the most variable gene families: the Major Histocompatibility Complex genes (MHC), and Olfa...
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Alma Mater Studiorum - Università di Bologna
2016
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Online Access: | http://amsdottorato.unibo.it/7445/ http://amsdottorato.unibo.it/7445/1/Tesi_dottorato_LAPALOMBELLA_ciclo28.pdf |
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ftunivbologntesi:oai:amsdottorato.cib.unibo.it:7445 2023-05-15T15:49:33+02:00 From Sanger to NGS: Detecting MHC (Major Histocompatibility Complex) Class II and OR (Olfactory Receptors) Genetic Variability in Italian Wolves (Canis Lupus) and relative Canids Lapalombella, Silvana <1982> Randi, Ettore 2016-05-17 application/pdf http://amsdottorato.unibo.it/7445/ http://amsdottorato.unibo.it/7445/1/Tesi_dottorato_LAPALOMBELLA_ciclo28.pdf en eng Alma Mater Studiorum - Università di Bologna http://amsdottorato.unibo.it/7445/1/Tesi_dottorato_LAPALOMBELLA_ciclo28.pdf urn:nbn:it:unibo-18548 Lapalombella, Silvana (2016) From Sanger to NGS: Detecting MHC (Major Histocompatibility Complex) Class II and OR (Olfactory Receptors) Genetic Variability in Italian Wolves (Canis Lupus) and relative Canids, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Biodiversità ed evoluzione <http://amsdottorato.unibo.it/view/dottorati/DOT452/>, 28 Ciclo. DOI 10.6092/unibo/amsdottorato/7445. info:eu-repo/semantics/openAccess BIO/05 Zoologia Doctoral Thesis PeerReviewed 2016 ftunivbologntesi 2021-06-03T13:39:42Z In this PhD thesis I will describe different aspects of conservation genetics and genomics of two wild Canidae species, the wolf (Canis lupus) and the golden jackal (Canis aureus), through the study of two of the most variable gene families: the Major Histocompatibility Complex genes (MHC), and Olfactory Receptors genes (OR). In order to perform these studies both Sanger and next generation sequencing (NGS) DNA techniques have been used. The background of the thesis is described in the “General introduction” with phylogeny, classification and evolutionary ecology of the Canidae, with a focus on the species Canis lupus and its main conservation concerns in Italy. Moreover, I will introduce the importance to perform genetic studies as tools for wild-life conservation and management, with a description of the framework of the principal historical and currently used molecular markers that had driven to develop MHC and OR sequencing projects. The thesis is divided into two parts, “PART I – The MHC typing project” and “PART II – The OR genes typing project”. A total of four scientific papers (already published or under revision) will be introduced and illustrated as result of three years of PhD activities at ISPRA’s (Istituto Superiore per la Protezione e la Ricerca Ambientale), Laboratory of conservation genetics, in Ozzano dell’Emilia, and thankfully to a PhD fellowship granted by the Università di Bologna. Doctoral or Postdoctoral Thesis Canis lupus Università di Bologna: AMS Tesi di Dottorato (Alm@DL) |
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Università di Bologna: AMS Tesi di Dottorato (Alm@DL) |
op_collection_id |
ftunivbologntesi |
language |
English |
topic |
BIO/05 Zoologia |
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BIO/05 Zoologia Lapalombella, Silvana <1982> From Sanger to NGS: Detecting MHC (Major Histocompatibility Complex) Class II and OR (Olfactory Receptors) Genetic Variability in Italian Wolves (Canis Lupus) and relative Canids |
topic_facet |
BIO/05 Zoologia |
description |
In this PhD thesis I will describe different aspects of conservation genetics and genomics of two wild Canidae species, the wolf (Canis lupus) and the golden jackal (Canis aureus), through the study of two of the most variable gene families: the Major Histocompatibility Complex genes (MHC), and Olfactory Receptors genes (OR). In order to perform these studies both Sanger and next generation sequencing (NGS) DNA techniques have been used. The background of the thesis is described in the “General introduction” with phylogeny, classification and evolutionary ecology of the Canidae, with a focus on the species Canis lupus and its main conservation concerns in Italy. Moreover, I will introduce the importance to perform genetic studies as tools for wild-life conservation and management, with a description of the framework of the principal historical and currently used molecular markers that had driven to develop MHC and OR sequencing projects. The thesis is divided into two parts, “PART I – The MHC typing project” and “PART II – The OR genes typing project”. A total of four scientific papers (already published or under revision) will be introduced and illustrated as result of three years of PhD activities at ISPRA’s (Istituto Superiore per la Protezione e la Ricerca Ambientale), Laboratory of conservation genetics, in Ozzano dell’Emilia, and thankfully to a PhD fellowship granted by the Università di Bologna. |
author2 |
Randi, Ettore |
format |
Doctoral or Postdoctoral Thesis |
author |
Lapalombella, Silvana <1982> |
author_facet |
Lapalombella, Silvana <1982> |
author_sort |
Lapalombella, Silvana <1982> |
title |
From Sanger to NGS: Detecting MHC (Major Histocompatibility Complex) Class II and OR (Olfactory Receptors) Genetic Variability in Italian Wolves (Canis Lupus) and relative Canids |
title_short |
From Sanger to NGS: Detecting MHC (Major Histocompatibility Complex) Class II and OR (Olfactory Receptors) Genetic Variability in Italian Wolves (Canis Lupus) and relative Canids |
title_full |
From Sanger to NGS: Detecting MHC (Major Histocompatibility Complex) Class II and OR (Olfactory Receptors) Genetic Variability in Italian Wolves (Canis Lupus) and relative Canids |
title_fullStr |
From Sanger to NGS: Detecting MHC (Major Histocompatibility Complex) Class II and OR (Olfactory Receptors) Genetic Variability in Italian Wolves (Canis Lupus) and relative Canids |
title_full_unstemmed |
From Sanger to NGS: Detecting MHC (Major Histocompatibility Complex) Class II and OR (Olfactory Receptors) Genetic Variability in Italian Wolves (Canis Lupus) and relative Canids |
title_sort |
from sanger to ngs: detecting mhc (major histocompatibility complex) class ii and or (olfactory receptors) genetic variability in italian wolves (canis lupus) and relative canids |
publisher |
Alma Mater Studiorum - Università di Bologna |
publishDate |
2016 |
url |
http://amsdottorato.unibo.it/7445/ http://amsdottorato.unibo.it/7445/1/Tesi_dottorato_LAPALOMBELLA_ciclo28.pdf |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
http://amsdottorato.unibo.it/7445/1/Tesi_dottorato_LAPALOMBELLA_ciclo28.pdf urn:nbn:it:unibo-18548 Lapalombella, Silvana (2016) From Sanger to NGS: Detecting MHC (Major Histocompatibility Complex) Class II and OR (Olfactory Receptors) Genetic Variability in Italian Wolves (Canis Lupus) and relative Canids, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Biodiversità ed evoluzione <http://amsdottorato.unibo.it/view/dottorati/DOT452/>, 28 Ciclo. DOI 10.6092/unibo/amsdottorato/7445. |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1766384585930702848 |