Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas.
Alveolar echinococcosis (AE) is a rare but severe disease that affects more than 18,000 people worldwide per year. The complete sequencing of the mitochondrial genome of Echinococcus multilocularis has made it possible to study the genetic diversity of the parasite and its spatial and temporal evolu...
Published in: | International Journal for Parasitology |
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2024
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Online Access: | https://boris.unibe.ch/197408/1/1-s2.0-S0020751924001103-main.pdf https://boris.unibe.ch/197408/ |
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ftunivbern:oai:boris.unibe.ch:197408 2024-06-23T07:50:32+00:00 Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas. Lallemand, Séverine Oyhenart, Jorge Valot, Benoit Borne, Romain Bohard, Louis Umhang, Gérald Karamon, Jacek Konyaev, Sergey Rönnberg, Caroline Gottstein, Bruno Weil-Verhoeven, Delphine Richou, Carine Bresson-Hadni, Solange Millon, Laurence Bellanger, Anne-Pauline Knapp, Jenny 2024-05-28 application/pdf https://boris.unibe.ch/197408/1/1-s2.0-S0020751924001103-main.pdf https://boris.unibe.ch/197408/ eng eng Elsevier https://boris.unibe.ch/197408/ info:eu-repo/semantics/openAccess Lallemand, Séverine; Oyhenart, Jorge; Valot, Benoit; Borne, Romain; Bohard, Louis; Umhang, Gérald; Karamon, Jacek; Konyaev, Sergey; Rönnberg, Caroline; Gottstein, Bruno; Weil-Verhoeven, Delphine; Richou, Carine; Bresson-Hadni, Solange; Millon, Laurence; Bellanger, Anne-Pauline; Knapp, Jenny (2024). Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas. (In Press). International journal for parasitology Elsevier 10.1016/j.ijpara.2024.05.004 <http://dx.doi.org/10.1016/j.ijpara.2024.05.004> 570 Life sciences biology 610 Medicine & health info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion PeerReviewed 2024 ftunivbern https://doi.org/10.1016/j.ijpara.2024.05.004 2024-06-04T14:16:20Z Alveolar echinococcosis (AE) is a rare but severe disease that affects more than 18,000 people worldwide per year. The complete sequencing of the mitochondrial genome of Echinococcus multilocularis has made it possible to study the genetic diversity of the parasite and its spatial and temporal evolution. We amplified the whole mitochondrial genome by PCR, using one uniplex and two multiplex reactions to cover the 13,738 bp of the mitogenome, and then sequenced the amplicons with Illumina technology. In total, 113 samples from Europe, Asia, , the Arctic and North America were analyzed. Three major haplogroups were found: HG1, which clustered samples from Alaska (including Saint-Lawrence Island), Yakutia (Russia) and Svalbard; HG2, with samples from Asia, North America and Europe; and HG3, subdivided into three micro-haplogroups. HG3a included samples from North America and Europe, whereas HG3b and HG3c only include samples from Europe. In France, HG3a included samples from patients more recently diagnosed in a region outside the historical endemic area. A fourth putative haplogroup, HG4, was represented by only one isolate from Olkhon Island (Russia). The increased discriminatory power of the complete sequencing of the E. multilocularis mitogenome has made it possible to highlight four distinct geographical clusters, one being divided into three micro-haplogroups in France. Article in Journal/Newspaper Arctic Svalbard Yakutia Alaska BORIS (Bern Open Repository and Information System, University of Bern) Arctic Svalbard Lawrence Island ENVELOPE(-103.718,-103.718,56.967,56.967) International Journal for Parasitology |
institution |
Open Polar |
collection |
BORIS (Bern Open Repository and Information System, University of Bern) |
op_collection_id |
ftunivbern |
language |
English |
topic |
570 Life sciences biology 610 Medicine & health |
spellingShingle |
570 Life sciences biology 610 Medicine & health Lallemand, Séverine Oyhenart, Jorge Valot, Benoit Borne, Romain Bohard, Louis Umhang, Gérald Karamon, Jacek Konyaev, Sergey Rönnberg, Caroline Gottstein, Bruno Weil-Verhoeven, Delphine Richou, Carine Bresson-Hadni, Solange Millon, Laurence Bellanger, Anne-Pauline Knapp, Jenny Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas. |
topic_facet |
570 Life sciences biology 610 Medicine & health |
description |
Alveolar echinococcosis (AE) is a rare but severe disease that affects more than 18,000 people worldwide per year. The complete sequencing of the mitochondrial genome of Echinococcus multilocularis has made it possible to study the genetic diversity of the parasite and its spatial and temporal evolution. We amplified the whole mitochondrial genome by PCR, using one uniplex and two multiplex reactions to cover the 13,738 bp of the mitogenome, and then sequenced the amplicons with Illumina technology. In total, 113 samples from Europe, Asia, , the Arctic and North America were analyzed. Three major haplogroups were found: HG1, which clustered samples from Alaska (including Saint-Lawrence Island), Yakutia (Russia) and Svalbard; HG2, with samples from Asia, North America and Europe; and HG3, subdivided into three micro-haplogroups. HG3a included samples from North America and Europe, whereas HG3b and HG3c only include samples from Europe. In France, HG3a included samples from patients more recently diagnosed in a region outside the historical endemic area. A fourth putative haplogroup, HG4, was represented by only one isolate from Olkhon Island (Russia). The increased discriminatory power of the complete sequencing of the E. multilocularis mitogenome has made it possible to highlight four distinct geographical clusters, one being divided into three micro-haplogroups in France. |
format |
Article in Journal/Newspaper |
author |
Lallemand, Séverine Oyhenart, Jorge Valot, Benoit Borne, Romain Bohard, Louis Umhang, Gérald Karamon, Jacek Konyaev, Sergey Rönnberg, Caroline Gottstein, Bruno Weil-Verhoeven, Delphine Richou, Carine Bresson-Hadni, Solange Millon, Laurence Bellanger, Anne-Pauline Knapp, Jenny |
author_facet |
Lallemand, Séverine Oyhenart, Jorge Valot, Benoit Borne, Romain Bohard, Louis Umhang, Gérald Karamon, Jacek Konyaev, Sergey Rönnberg, Caroline Gottstein, Bruno Weil-Verhoeven, Delphine Richou, Carine Bresson-Hadni, Solange Millon, Laurence Bellanger, Anne-Pauline Knapp, Jenny |
author_sort |
Lallemand, Séverine |
title |
Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas. |
title_short |
Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas. |
title_full |
Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas. |
title_fullStr |
Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas. |
title_full_unstemmed |
Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas. |
title_sort |
challenging the phylogenetic relationships among echinococcus multilocularis isolates from main endemic areas. |
publisher |
Elsevier |
publishDate |
2024 |
url |
https://boris.unibe.ch/197408/1/1-s2.0-S0020751924001103-main.pdf https://boris.unibe.ch/197408/ |
long_lat |
ENVELOPE(-103.718,-103.718,56.967,56.967) |
geographic |
Arctic Svalbard Lawrence Island |
geographic_facet |
Arctic Svalbard Lawrence Island |
genre |
Arctic Svalbard Yakutia Alaska |
genre_facet |
Arctic Svalbard Yakutia Alaska |
op_source |
Lallemand, Séverine; Oyhenart, Jorge; Valot, Benoit; Borne, Romain; Bohard, Louis; Umhang, Gérald; Karamon, Jacek; Konyaev, Sergey; Rönnberg, Caroline; Gottstein, Bruno; Weil-Verhoeven, Delphine; Richou, Carine; Bresson-Hadni, Solange; Millon, Laurence; Bellanger, Anne-Pauline; Knapp, Jenny (2024). Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas. (In Press). International journal for parasitology Elsevier 10.1016/j.ijpara.2024.05.004 <http://dx.doi.org/10.1016/j.ijpara.2024.05.004> |
op_relation |
https://boris.unibe.ch/197408/ |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.ijpara.2024.05.004 |
container_title |
International Journal for Parasitology |
_version_ |
1802641438374100992 |