Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment
The S segment of bank vole (Clethrionomys glareolus)-associated Puumala orthohantavirus (PUUV) contains two overlapping open reading frames coding for the nucleocapsid (N) and a non-structural (NSs) protein. To identify the influence of bank vole population dynamics on PUUV S segment sequence evolut...
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ftunivbern:oai:boris.unibe.ch:146071 2023-08-20T04:07:59+02:00 Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment Binder, Florian Ryll, René Drewes, Stephan Jagdmann, Sandra Reil, Daniela Hiltbrunner, Melanie Rosenfeld, Ulrike M. Imholt, Christian Jacob, Jens Heckel, Gerald Ulrich, Rainer G. 2020-07-08 application/pdf https://boris.unibe.ch/146071/1/pathogens-09-00548-v3.pdf https://boris.unibe.ch/146071/ eng eng MDPI AG https://boris.unibe.ch/146071/ info:eu-repo/semantics/openAccess Binder, Florian; Ryll, René; Drewes, Stephan; Jagdmann, Sandra; Reil, Daniela; Hiltbrunner, Melanie; Rosenfeld, Ulrike M.; Imholt, Christian; Jacob, Jens; Heckel, Gerald; Ulrich, Rainer G. (2020). Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment. Pathogens, 9(7) MDPI AG 10.3390/pathogens9070548 <http://dx.doi.org/10.3390/pathogens9070548> 570 Life sciences biology info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion PeerReviewed 2020 ftunivbern https://doi.org/10.3390/pathogens9070548 2023-07-31T22:01:18Z The S segment of bank vole (Clethrionomys glareolus)-associated Puumala orthohantavirus (PUUV) contains two overlapping open reading frames coding for the nucleocapsid (N) and a non-structural (NSs) protein. To identify the influence of bank vole population dynamics on PUUV S segment sequence evolution and test for spillover infections in sympatric rodent species, during 2010–2014, 883 bank voles, 357 yellow-necked mice (Apodemus flavicollis), 62 wood mice (A. sylvaticus), 149 common voles (Microtus arvalis) and 8 field voles (M. agrestis) were collected in Baden-Wuerttemberg and North Rhine-Westphalia, Germany. In total, 27.9% and 22.3% of bank voles were positive for PUUV-reactive antibodies and PUUV-specific RNA, respectively. One of eight field voles was PUUV RNA-positive, indicating a spillover infection, but none of the other species showed evidence of PUUV infection. Phylogenetic and isolation-by-distance analyses demonstrated a spatial clustering of PUUV S segment sequences. In the hantavirus outbreak years 2010 and 2012, PUUV RNA prevalence was higher in our study regions compared to non-outbreak years 2011, 2013 and 2014. NSs amino acid and nucleotide sequence types showed temporal and/or local variation, whereas the N protein was highly conserved in the NSs overlapping region and, to a lower rate, in the N alone coding part. Article in Journal/Newspaper Microtus arvalis BORIS (Bern Open Repository and Information System, University of Bern) Pathogens 9 7 548 |
institution |
Open Polar |
collection |
BORIS (Bern Open Repository and Information System, University of Bern) |
op_collection_id |
ftunivbern |
language |
English |
topic |
570 Life sciences biology |
spellingShingle |
570 Life sciences biology Binder, Florian Ryll, René Drewes, Stephan Jagdmann, Sandra Reil, Daniela Hiltbrunner, Melanie Rosenfeld, Ulrike M. Imholt, Christian Jacob, Jens Heckel, Gerald Ulrich, Rainer G. Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment |
topic_facet |
570 Life sciences biology |
description |
The S segment of bank vole (Clethrionomys glareolus)-associated Puumala orthohantavirus (PUUV) contains two overlapping open reading frames coding for the nucleocapsid (N) and a non-structural (NSs) protein. To identify the influence of bank vole population dynamics on PUUV S segment sequence evolution and test for spillover infections in sympatric rodent species, during 2010–2014, 883 bank voles, 357 yellow-necked mice (Apodemus flavicollis), 62 wood mice (A. sylvaticus), 149 common voles (Microtus arvalis) and 8 field voles (M. agrestis) were collected in Baden-Wuerttemberg and North Rhine-Westphalia, Germany. In total, 27.9% and 22.3% of bank voles were positive for PUUV-reactive antibodies and PUUV-specific RNA, respectively. One of eight field voles was PUUV RNA-positive, indicating a spillover infection, but none of the other species showed evidence of PUUV infection. Phylogenetic and isolation-by-distance analyses demonstrated a spatial clustering of PUUV S segment sequences. In the hantavirus outbreak years 2010 and 2012, PUUV RNA prevalence was higher in our study regions compared to non-outbreak years 2011, 2013 and 2014. NSs amino acid and nucleotide sequence types showed temporal and/or local variation, whereas the N protein was highly conserved in the NSs overlapping region and, to a lower rate, in the N alone coding part. |
format |
Article in Journal/Newspaper |
author |
Binder, Florian Ryll, René Drewes, Stephan Jagdmann, Sandra Reil, Daniela Hiltbrunner, Melanie Rosenfeld, Ulrike M. Imholt, Christian Jacob, Jens Heckel, Gerald Ulrich, Rainer G. |
author_facet |
Binder, Florian Ryll, René Drewes, Stephan Jagdmann, Sandra Reil, Daniela Hiltbrunner, Melanie Rosenfeld, Ulrike M. Imholt, Christian Jacob, Jens Heckel, Gerald Ulrich, Rainer G. |
author_sort |
Binder, Florian |
title |
Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment |
title_short |
Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment |
title_full |
Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment |
title_fullStr |
Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment |
title_full_unstemmed |
Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment |
title_sort |
spatial and temporal evolutionary patterns in puumala orthohantavirus (puuv) s segment |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://boris.unibe.ch/146071/1/pathogens-09-00548-v3.pdf https://boris.unibe.ch/146071/ |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_source |
Binder, Florian; Ryll, René; Drewes, Stephan; Jagdmann, Sandra; Reil, Daniela; Hiltbrunner, Melanie; Rosenfeld, Ulrike M.; Imholt, Christian; Jacob, Jens; Heckel, Gerald; Ulrich, Rainer G. (2020). Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment. Pathogens, 9(7) MDPI AG 10.3390/pathogens9070548 <http://dx.doi.org/10.3390/pathogens9070548> |
op_relation |
https://boris.unibe.ch/146071/ |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/pathogens9070548 |
container_title |
Pathogens |
container_volume |
9 |
container_issue |
7 |
container_start_page |
548 |
_version_ |
1774719992853430272 |