Increased IL-4 and decreased regulatory cytokine production following relocation of Icelandic horses from a high to low endoparasite environment
Insect bite hypersensitivity (IBH) is an IgE-mediated dermatitis of horses caused by bites of Culicoides spp. IBH does not occur in Iceland where Culicoides are absent. However, following importation into continental Europe where Culicoides are present, >or=50% of Icelandic horses (1st generation...
Published in: | Veterinary Immunology and Immunopathology |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier
2010
|
Subjects: | |
Online Access: | https://boris.unibe.ch/12376/ |
id |
ftunivbern:oai:boris.unibe.ch:12376 |
---|---|
record_format |
openpolar |
spelling |
ftunivbern:oai:boris.unibe.ch:12376 2023-08-20T04:07:18+02:00 Increased IL-4 and decreased regulatory cytokine production following relocation of Icelandic horses from a high to low endoparasite environment Hamza, Eman Torsteinsdottir, S. Eydal, M. Frey, Caroline Mirkovitch, Jelena Brcic, Marija Wagner, B. Wilson, A.D. Jungi, Thomas Marti, Eliane Isabelle 2010 https://boris.unibe.ch/12376/ eng eng Elsevier https://boris.unibe.ch/12376/ info:eu-repo/semantics/restrictedAccess Hamza, Eman; Torsteinsdottir, S.; Eydal, M.; Frey, Caroline; Mirkovitch, Jelena; Brcic, Marija; Wagner, B.; Wilson, A.D.; Jungi, Thomas; Marti, Eliane Isabelle (2010). Increased IL-4 and decreased regulatory cytokine production following relocation of Icelandic horses from a high to low endoparasite environment. Veterinary immunology and immunopathology, 133(1), pp. 40-50. Amsterdam: Elsevier 10.1016/j.vetimm.2009.07.002 <http://dx.doi.org/10.1016/j.vetimm.2009.07.002> 630 Agriculture info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion PeerReviewed 2010 ftunivbern https://doi.org/10.1016/j.vetimm.2009.07.002 2023-07-31T20:36:05Z Insect bite hypersensitivity (IBH) is an IgE-mediated dermatitis of horses caused by bites of Culicoides spp. IBH does not occur in Iceland where Culicoides are absent. However, following importation into continental Europe where Culicoides are present, >or=50% of Icelandic horses (1st generation) develop IBH but <or=10% of their offspring born in Europe (2nd generation) do so. Recently, we showed that PBMC from 1st generation horses produce more IL-4 than 2nd generation horses. Since helminths and allergens induce Th2 responses, we investigated whether horses domiciled in Iceland are Th2-biased, and whether this is determined by helminth infection. We compared the parasite burden and T cell responses between Icelandic horses living in Iceland or Switzerland. Horses in Iceland have higher faecal egg counts, higher tapeworm-specific IgG(T) levels and higher total serum IgE levels than horses in Switzerland. Nevertheless, horses in Iceland displayed a low proportion of IL-4-producing cells in PBMC cultures after polyclonal or parasite extracts stimulation. No IL-4-producing cells were found in PBMC from horses after stimulation by Culicoides extract. Addition of anti-IL-10 and anti-TGF-beta1 to PBMC cultures of horses in Iceland increased the proportion of IL-4-producing cells after polyclonal or parasite antigens stimulation but not stimulation with Culicoides extract. This paralleled the high levels of IL-10 and TGF-beta1 found in supernatants from PBMC cultures of horses in Iceland. Collectively, horses living in Iceland have a high parasite burden but low IL-4 production. This supports the hypothesis that heavy helminth infections have a suppressive effect on IL-4 production mediated by IL-10 and TGF-beta1. Article in Journal/Newspaper Iceland BORIS (Bern Open Repository and Information System, University of Bern) Veterinary Immunology and Immunopathology 133 1 40 50 |
institution |
Open Polar |
collection |
BORIS (Bern Open Repository and Information System, University of Bern) |
op_collection_id |
ftunivbern |
language |
English |
topic |
630 Agriculture |
spellingShingle |
630 Agriculture Hamza, Eman Torsteinsdottir, S. Eydal, M. Frey, Caroline Mirkovitch, Jelena Brcic, Marija Wagner, B. Wilson, A.D. Jungi, Thomas Marti, Eliane Isabelle Increased IL-4 and decreased regulatory cytokine production following relocation of Icelandic horses from a high to low endoparasite environment |
topic_facet |
630 Agriculture |
description |
Insect bite hypersensitivity (IBH) is an IgE-mediated dermatitis of horses caused by bites of Culicoides spp. IBH does not occur in Iceland where Culicoides are absent. However, following importation into continental Europe where Culicoides are present, >or=50% of Icelandic horses (1st generation) develop IBH but <or=10% of their offspring born in Europe (2nd generation) do so. Recently, we showed that PBMC from 1st generation horses produce more IL-4 than 2nd generation horses. Since helminths and allergens induce Th2 responses, we investigated whether horses domiciled in Iceland are Th2-biased, and whether this is determined by helminth infection. We compared the parasite burden and T cell responses between Icelandic horses living in Iceland or Switzerland. Horses in Iceland have higher faecal egg counts, higher tapeworm-specific IgG(T) levels and higher total serum IgE levels than horses in Switzerland. Nevertheless, horses in Iceland displayed a low proportion of IL-4-producing cells in PBMC cultures after polyclonal or parasite extracts stimulation. No IL-4-producing cells were found in PBMC from horses after stimulation by Culicoides extract. Addition of anti-IL-10 and anti-TGF-beta1 to PBMC cultures of horses in Iceland increased the proportion of IL-4-producing cells after polyclonal or parasite antigens stimulation but not stimulation with Culicoides extract. This paralleled the high levels of IL-10 and TGF-beta1 found in supernatants from PBMC cultures of horses in Iceland. Collectively, horses living in Iceland have a high parasite burden but low IL-4 production. This supports the hypothesis that heavy helminth infections have a suppressive effect on IL-4 production mediated by IL-10 and TGF-beta1. |
format |
Article in Journal/Newspaper |
author |
Hamza, Eman Torsteinsdottir, S. Eydal, M. Frey, Caroline Mirkovitch, Jelena Brcic, Marija Wagner, B. Wilson, A.D. Jungi, Thomas Marti, Eliane Isabelle |
author_facet |
Hamza, Eman Torsteinsdottir, S. Eydal, M. Frey, Caroline Mirkovitch, Jelena Brcic, Marija Wagner, B. Wilson, A.D. Jungi, Thomas Marti, Eliane Isabelle |
author_sort |
Hamza, Eman |
title |
Increased IL-4 and decreased regulatory cytokine production following relocation of Icelandic horses from a high to low endoparasite environment |
title_short |
Increased IL-4 and decreased regulatory cytokine production following relocation of Icelandic horses from a high to low endoparasite environment |
title_full |
Increased IL-4 and decreased regulatory cytokine production following relocation of Icelandic horses from a high to low endoparasite environment |
title_fullStr |
Increased IL-4 and decreased regulatory cytokine production following relocation of Icelandic horses from a high to low endoparasite environment |
title_full_unstemmed |
Increased IL-4 and decreased regulatory cytokine production following relocation of Icelandic horses from a high to low endoparasite environment |
title_sort |
increased il-4 and decreased regulatory cytokine production following relocation of icelandic horses from a high to low endoparasite environment |
publisher |
Elsevier |
publishDate |
2010 |
url |
https://boris.unibe.ch/12376/ |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Hamza, Eman; Torsteinsdottir, S.; Eydal, M.; Frey, Caroline; Mirkovitch, Jelena; Brcic, Marija; Wagner, B.; Wilson, A.D.; Jungi, Thomas; Marti, Eliane Isabelle (2010). Increased IL-4 and decreased regulatory cytokine production following relocation of Icelandic horses from a high to low endoparasite environment. Veterinary immunology and immunopathology, 133(1), pp. 40-50. Amsterdam: Elsevier 10.1016/j.vetimm.2009.07.002 <http://dx.doi.org/10.1016/j.vetimm.2009.07.002> |
op_relation |
https://boris.unibe.ch/12376/ |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.vetimm.2009.07.002 |
container_title |
Veterinary Immunology and Immunopathology |
container_volume |
133 |
container_issue |
1 |
container_start_page |
40 |
op_container_end_page |
50 |
_version_ |
1774718835380715520 |