Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries
Rat hepatitis E virus (HEV) is genetically only distantly related to hepeviruses found in other mammalian reservoirs and in humans. It was initially detected in Norway rats (Rattus norvegicus) from Germany, and subsequently in rats from Vietnam, the USA, Indonesia, China, Denmark and France. Here, w...
Published in: | Veterinary Microbiology |
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ftunivbern:oai:boris.unibe.ch:118916 2023-08-20T04:09:25+02:00 Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries Ryll, René Bernstein, Samuel Heuser, Elisa Schlegel, Mathias Dremsek, Paul Zumpe, Maxi Wolf, Sandro Pépin, Michel Bajomi, Daniel Müller, Gabi Heiberg, Ann-Charlotte Spahr, Carina Lang, Johannes Groschup, Martin H. Ansorge, Hermann Freise, Jona Guenther, Sebastian Baert, Kristof Ruiz-Fons, Francisco Pikula, Jiri Knap, Natasa Tsakmakidis, Ioannis Dovas, Chrysostomos Zanet, Stefania Imholt, Christian Heckel, Gerald Johne, Reimar Ulrich, Rainer G. 2017-07-02 application/pdf https://boris.unibe.ch/118916/1/1-s2.0-S0378113516302449-main.pdf https://boris.unibe.ch/118916/ eng eng Elsevier https://boris.unibe.ch/118916/ info:eu-repo/semantics/restrictedAccess Ryll, René; Bernstein, Samuel; Heuser, Elisa; Schlegel, Mathias; Dremsek, Paul; Zumpe, Maxi; Wolf, Sandro; Pépin, Michel; Bajomi, Daniel; Müller, Gabi; Heiberg, Ann-Charlotte; Spahr, Carina; Lang, Johannes; Groschup, Martin H.; Ansorge, Hermann; Freise, Jona; Guenther, Sebastian; Baert, Kristof; Ruiz-Fons, Francisco; Pikula, Jiri; . (2017). Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries. Veterinary microbiology, 208(208), pp. 58-68. Elsevier 10.1016/j.vetmic.2017.07.001 <http://dx.doi.org/10.1016/j.vetmic.2017.07.001> 570 Life sciences biology info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion PeerReviewed 2017 ftunivbern https://doi.org/10.1016/j.vetmic.2017.07.001 2023-07-31T21:45:46Z Rat hepatitis E virus (HEV) is genetically only distantly related to hepeviruses found in other mammalian reservoirs and in humans. It was initially detected in Norway rats (Rattus norvegicus) from Germany, and subsequently in rats from Vietnam, the USA, Indonesia, China, Denmark and France. Here, we report on a molecular survey of Norway rats and Black rats (Rattus rattus) from 12 European countries for ratHEV and human pathogenic hepeviruses. RatHEV-specific real-time and conventional RT-PCR investigations revealed the presence of ratHEV in 63 of 508 (12.4%) rats at the majority of sites in 11 of 12 countries. In contrast, a real-time RT-PCR specific for human pathogenic HEV genotypes 1–4 and a nested broadspectrum (NBS) RT-PCR with subsequent sequence determination did not detect any infections with these genotypes. Only in a single Norway rat from Belgium a rabbit HEV-like genotype 3 sequence was detected. Phylogenetic analysis indicated a clustering of all other novel Norway and Black rat-derived sequences with ratHEV sequences from Europe, the USA and a Black rat-derived sequence from Indonesia within the proposed ratHEV genotype 1. No difference in infection status was detected related to age, sex, rat species or density of human settlements and zoological gardens. In conclusion, our investigation shows a broad geographical distribution of ratHEV in Norway and Black rats from Europe and its presence in all settlement types investigated. Article in Journal/Newspaper Rattus rattus BORIS (Bern Open Repository and Information System, University of Bern) Norway Veterinary Microbiology 208 58 68 |
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Open Polar |
collection |
BORIS (Bern Open Repository and Information System, University of Bern) |
op_collection_id |
ftunivbern |
language |
English |
topic |
570 Life sciences biology |
spellingShingle |
570 Life sciences biology Ryll, René Bernstein, Samuel Heuser, Elisa Schlegel, Mathias Dremsek, Paul Zumpe, Maxi Wolf, Sandro Pépin, Michel Bajomi, Daniel Müller, Gabi Heiberg, Ann-Charlotte Spahr, Carina Lang, Johannes Groschup, Martin H. Ansorge, Hermann Freise, Jona Guenther, Sebastian Baert, Kristof Ruiz-Fons, Francisco Pikula, Jiri Knap, Natasa Tsakmakidis, Ioannis Dovas, Chrysostomos Zanet, Stefania Imholt, Christian Heckel, Gerald Johne, Reimar Ulrich, Rainer G. Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries |
topic_facet |
570 Life sciences biology |
description |
Rat hepatitis E virus (HEV) is genetically only distantly related to hepeviruses found in other mammalian reservoirs and in humans. It was initially detected in Norway rats (Rattus norvegicus) from Germany, and subsequently in rats from Vietnam, the USA, Indonesia, China, Denmark and France. Here, we report on a molecular survey of Norway rats and Black rats (Rattus rattus) from 12 European countries for ratHEV and human pathogenic hepeviruses. RatHEV-specific real-time and conventional RT-PCR investigations revealed the presence of ratHEV in 63 of 508 (12.4%) rats at the majority of sites in 11 of 12 countries. In contrast, a real-time RT-PCR specific for human pathogenic HEV genotypes 1–4 and a nested broadspectrum (NBS) RT-PCR with subsequent sequence determination did not detect any infections with these genotypes. Only in a single Norway rat from Belgium a rabbit HEV-like genotype 3 sequence was detected. Phylogenetic analysis indicated a clustering of all other novel Norway and Black rat-derived sequences with ratHEV sequences from Europe, the USA and a Black rat-derived sequence from Indonesia within the proposed ratHEV genotype 1. No difference in infection status was detected related to age, sex, rat species or density of human settlements and zoological gardens. In conclusion, our investigation shows a broad geographical distribution of ratHEV in Norway and Black rats from Europe and its presence in all settlement types investigated. |
format |
Article in Journal/Newspaper |
author |
Ryll, René Bernstein, Samuel Heuser, Elisa Schlegel, Mathias Dremsek, Paul Zumpe, Maxi Wolf, Sandro Pépin, Michel Bajomi, Daniel Müller, Gabi Heiberg, Ann-Charlotte Spahr, Carina Lang, Johannes Groschup, Martin H. Ansorge, Hermann Freise, Jona Guenther, Sebastian Baert, Kristof Ruiz-Fons, Francisco Pikula, Jiri Knap, Natasa Tsakmakidis, Ioannis Dovas, Chrysostomos Zanet, Stefania Imholt, Christian Heckel, Gerald Johne, Reimar Ulrich, Rainer G. |
author_facet |
Ryll, René Bernstein, Samuel Heuser, Elisa Schlegel, Mathias Dremsek, Paul Zumpe, Maxi Wolf, Sandro Pépin, Michel Bajomi, Daniel Müller, Gabi Heiberg, Ann-Charlotte Spahr, Carina Lang, Johannes Groschup, Martin H. Ansorge, Hermann Freise, Jona Guenther, Sebastian Baert, Kristof Ruiz-Fons, Francisco Pikula, Jiri Knap, Natasa Tsakmakidis, Ioannis Dovas, Chrysostomos Zanet, Stefania Imholt, Christian Heckel, Gerald Johne, Reimar Ulrich, Rainer G. |
author_sort |
Ryll, René |
title |
Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries |
title_short |
Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries |
title_full |
Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries |
title_fullStr |
Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries |
title_full_unstemmed |
Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries |
title_sort |
detection of rat hepatitis e virus in wild norway rats (rattus norvegicus) and black rats (rattus rattus) from 11 european countries |
publisher |
Elsevier |
publishDate |
2017 |
url |
https://boris.unibe.ch/118916/1/1-s2.0-S0378113516302449-main.pdf https://boris.unibe.ch/118916/ |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Ryll, René; Bernstein, Samuel; Heuser, Elisa; Schlegel, Mathias; Dremsek, Paul; Zumpe, Maxi; Wolf, Sandro; Pépin, Michel; Bajomi, Daniel; Müller, Gabi; Heiberg, Ann-Charlotte; Spahr, Carina; Lang, Johannes; Groschup, Martin H.; Ansorge, Hermann; Freise, Jona; Guenther, Sebastian; Baert, Kristof; Ruiz-Fons, Francisco; Pikula, Jiri; . (2017). Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries. Veterinary microbiology, 208(208), pp. 58-68. Elsevier 10.1016/j.vetmic.2017.07.001 <http://dx.doi.org/10.1016/j.vetmic.2017.07.001> |
op_relation |
https://boris.unibe.ch/118916/ |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.vetmic.2017.07.001 |
container_title |
Veterinary Microbiology |
container_volume |
208 |
container_start_page |
58 |
op_container_end_page |
68 |
_version_ |
1774722357457321984 |